{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:05:21Z","timestamp":1773795921100,"version":"3.50.1"},"reference-count":74,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,9,2]]},"abstract":"<jats:p>La Crosse virus (LACV), a leading cause of arboviral pediatric encephalitis in the United States, is emerging in Appalachia. Here, we report field and laboratory evidence that suggest LACV may be using <jats:italic>Culex<\/jats:italic> mosquitoes as additional vectors in this region. This bunyavirus was detected by reverse-transcriptase polymerase chain reaction in two pools of <jats:italic>Culex<\/jats:italic> mosquitoes in southwestern Virginia and in six pools in West Virginia. To assess vector competence, we offered LACV blood meals to field-collected <jats:italic>Culex restuans<\/jats:italic> Theobald, <jats:italic>Cx. pipiens<\/jats:italic> L., and <jats:italic>Aedes triseriatus<\/jats:italic> (Say). Both <jats:italic>Culex<\/jats:italic> species were susceptible to infection. LACV-positive salivary expectorate, indicative of the ability to transmit, was detected in a small proportion of <jats:italic>Cx. restuans<\/jats:italic> (9%) and <jats:italic>Cx. pipiens<\/jats:italic> (4%) compared with <jats:italic>Ae. triseriatus<\/jats:italic> (40%). In a companion study of <jats:italic>Cx. restuans<\/jats:italic> only, we found that adults derived from nutritionally stressed larvae were significantly more likely to disseminate and transmit LACV. Our results indicate a potential role of <jats:italic>Culex<\/jats:italic> spp. in LACV dynamics that should be explored further in endemic areas.<\/jats:p>","DOI":"10.4269\/ajtmh.14-0128","type":"journal-article","created":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T01:31:01Z","timestamp":1436923861000},"page":"461-467","source":"Crossref","is-referenced-by-count":19,"title":["La Crosse Virus Field Detection and Vector Competence of Culex Mosquitoes"],"prefix":"10.4269","volume":"93","author":[{"given":"M. Camille","family":"Harris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorian M.","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric J.","family":"Dotseth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally L.","family":"Paulson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana M.","family":"Hawley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Emerg Infect Dis","first-page":"807","article-title":"First isolation of La Crosse virus from naturally infected Aedes albopictus","volume":"7","author":"Gerhardt","year":"2001"},{"key":"ref2","series-title":"J Am Mosq Control Assoc","first-page":"115","article-title":"Polymerase chain reaction assay identifies Culex nigripalpus: part of an assay for molecular identification of the common Culex (Culex) mosquitoes of the eastern United States","volume":"19","author":"Aspen","year":"2003"},{"key":"ref3","series-title":"Am J Trop Med Hyg","first-page":"339","article-title":"Rapid assays for identification of members of the Culex (Culex) pipiens complex, their hybrids, and other sibling species (Diptera: Culicidae)","volume":"70","author":"Smith","year":"2004"},{"key":"ref4","series-title":"Am J Trop Med Hyg","first-page":"15","article-title":"Multiplex PCR assay for the detection and simultaneous differentiation of container-inhabiting Aedes mosquitoes in La Crosse virus endemic areas","volume":"81","author":"Byrd","year":"2009"},{"key":"ref5","series-title":"J Med Entomol","first-page":"884","article-title":"Potential for North American mosquitoes (Diptera: Culicidae) to transmit Rift Valley Fever virus","volume":"47","author":"Turell","year":"2010"},{"key":"ref6","series-title":"Annu Rev Entomol","first-page":"229","article-title":"Intrinsic factors affecting vector competence of mosquitoes for arboviruses","volume":"28","author":"Hardy","year":"1983"},{"key":"ref7","series-title":"Int J Environ Res Public Health","first-page":"249","article-title":"Nature, nurture and evolution of intra-species variation in mosquito arbovirus transmission competence","volume":"10","author":"Tabachnick","year":"2013"},{"key":"ref8","series-title":"Infect Dis Clin North Am","first-page":"83","article-title":"California La Crosse encephalitis","volume":"12","author":"McJunkin","year":"1998"},{"key":"ref9","series-title":"Am J Trop Med Hyg","first-page":"974","article-title":"Epidemiology of neuroinvasive arboviral disease in the United States, 1999\u20132007","volume":"79","author":"Reimann","year":"2008"},{"key":"ref10","series-title":"BMC Infect Dis","first-page":"9","article-title":"The spatial epidemiology and clinical features of reported cases of La Crosse Virus infection in West Virginia from 2003 to 2007","volume":"11","author":"Haddow","year":"2011"},{"key":"ref11","series-title":"Am J Epidemiol","first-page":"245","article-title":"Isolation of California encephalitis group virus from a fatal human illness","volume":"81","author":"Thompson","year":"1965"},{"key":"ref12","series-title":"Confirmed and Probable California Serogroup Virus Neuroinvasive Disease Cases, Human, United States, 1964\u20132010, By State (as of 5\/9\/2011)","author":"Centers for Disease Control and Prevention","year":"2011"},{"key":"ref13","series-title":"J Med Entomol","first-page":"403","article-title":"Habitat preferences and phenology of Ochlerotatus triseriatus and Aedes albopictus (Diptera: Culicidae) in southwestern Virginia","volume":"40","author":"Barker","year":"2003"},{"key":"ref14","series-title":"PLoS One","first-page":"e25739","article-title":"The demographic and socioeconomic factors predictive for populations at high-risk for La Crosse Virus infection in West Virginia","volume":"6","author":"Haddow","year":"2011"},{"key":"ref15","series-title":"J Med Entomol","first-page":"437","article-title":"Vertical transmission of La Crosse virus (California encephalitis group)\u2014transovarial and filial infection rates in Aedes triseriatus (Diptera: Culicidae)","volume":"14","author":"Miller","year":"1977"},{"key":"ref16","series-title":"Science","first-page":"530","article-title":"Venereal transmission of La Crosse (California encephalitis) arbovirus in Aedes triseriatus mosquitoes","volume":"196","author":"Thompson","year":"1977"},{"key":"ref17","series-title":"Am J Trop Med Hyg","first-page":"694","article-title":"Overwintering of La Crosse virus in Aedes triseriatus","volume":"23","author":"Watts","year":"1974"},{"key":"ref18","series-title":"Curr Top Microbiol Immunol","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/978-3-642-76018-1_2","article-title":"Bunyaviridae\u2014natural history","volume":"169","author":"Beaty","year":"1991"},{"key":"ref19","series-title":"J Med Entomol","first-page":"757","article-title":"Comparative potential of Aedes triseriatus, Aedes albopictus, and Aedes aegypti (Diptera: Culicidae) to transovarially transmit La Crosse virus","volume":"43","author":"Hughes","year":"2006"},{"key":"ref20","series-title":"J Med Entomol","first-page":"635","article-title":"Laboratory transmission of La Crosse virus by Ochlerotatus j. japonicus (Diptera: Culicidae)","volume":"39","author":"Sardelis","year":"2002"},{"key":"ref21","series-title":"Emerg Infect Dis","first-page":"856","article-title":"La Crosse virus in Aedes albopictus mosquitoes, Texas, USA, 2009","volume":"16","author":"Lambert","year":"2010"},{"key":"ref22","series-title":"J Med Entomol","first-page":"583","article-title":"Laboratory transmission of La Crosse encephalitis virus by several species of mosquitoes","volume":"10","author":"Watts","year":"1973"},{"key":"ref23","series-title":"J Am Mosq Control Assoc","first-page":"73","article-title":"Aedes canadensis, a vector of La Crosse virus (California serogroup) in Ohio","volume":"2","author":"Berry","year":"1986"},{"key":"ref24","series-title":"J Med Entomol","first-page":"559","article-title":"La Crosse encephalitis virus habitat associations in Nicholas County, West Virginia","volume":"37","author":"Nasci","year":"2000"},{"key":"ref25","series-title":"Am J Trop Med Hyg","first-page":"90","article-title":"La Crosse virus isolations from mosquitoes in Wisconsin, 1964\u20131968","volume":"21","author":"Thompson","year":"1972"},{"key":"ref26","series-title":"Am J Trop Med Hyg","first-page":"876","article-title":"Laboratory studies of transovarial transmission of La Crosse and other arboviruses by Aedes albopictus and Culex fatigans","volume":"24","author":"Tesh","year":"1975"},{"key":"ref27","series-title":"J Am Mosq Control Assoc","first-page":"10","article-title":"Culex pipiens: species versus species complex\u2014taxonomic history and perspective","volume":"28","author":"Harbach","year":"2012"},{"key":"ref28","series-title":"J Med Entomol","first-page":"249","article-title":"Aedes triseriatus (Diptera, Culicidae) and La Crosse virus III. Enhanced oral-transmission by nutrition-deprived mosquitoes","volume":"21","author":"Grimstad","year":"1984"},{"key":"ref29","series-title":"J Am Mosq Control Assoc","first-page":"170","article-title":"Effect of body size on the vector competence of field and laboratory populations of Aedes triseriatus for La Crosse virus","volume":"7","author":"Paulson","year":"1991"},{"key":"ref30","series-title":"J Am Mosq Control Assoc","first-page":"380","article-title":"Occurrence of tire inhabiting mosquito larvae in different geographic regions of West Virginia","volume":"21","author":"Joy","year":"2005"},{"key":"ref31","series-title":"J Am Mosq Control Assoc","first-page":"206","article-title":"Seasonal abundance of Culex restuans and Culex pipiens in southwestern Virginia through ovitrapping","volume":"22","author":"Jackson","year":"2006"},{"key":"ref32","series-title":"J Am Mosq Control Assoc","first-page":"360","article-title":"Oviposition preferences of Culex restuans and Culex pipiens (Diptera: Culicidae) for selected infusions in oviposition traps and gravid traps","volume":"21","author":"Jackson","year":"2005"},{"key":"ref33","series-title":"Ecology","first-page":"183","article-title":"Diversity-invasibility across an experimental disturbance gradient in Appalachian forests","volume":"89","author":"Belote","year":"2008"},{"key":"ref34","series-title":"Am J Trop Med Hyg","first-page":"1009","article-title":"Identification of Culex species by electrophoresis","volume":"26","author":"Saul","year":"1977"},{"key":"ref35","series-title":"J Med Entomol","first-page":"1","article-title":"Considerations for accurate identification of adult Culex restuans (Diptera: Culicidae) in field studies","volume":"45","author":"Harrington","year":"2008"},{"key":"ref36","series-title":"J Am Mosq Control Assoc","first-page":"294","article-title":"Comparison of Vero cell plaque assay, TaqMan reverse transcriptase polymerase chain reaction RNA assay, and VecTest antigen assay for detection of West Nile virus in field-collected mosquitoes","volume":"18","author":"Nasci","year":"2002"},{"key":"ref37","series-title":"J Clin Microbiol","first-page":"1885","article-title":"Nucleic acid amplification assays for detection of La Crosse virus RNA","volume":"43","author":"Lambert","year":"2005"},{"key":"ref38","series-title":"J Clin Microbiol","first-page":"1885","article-title":"Nucleic acid amplification assays for detection of La Crosse virus RNA","volume":"43","author":"Lambert","year":"2005"},{"key":"ref39","article-title":"La Crosse Virus in Southwestern Virginia: Role of Exotic Mosquito Species and Effect of Virus Infection on Feeding","author":"Jackson","year":"2009"},{"key":"ref40","series-title":"J Med Entomol","first-page":"1424","article-title":"La Crosse virus infection alters blood feeding behavior in Aedes triseriatus and Aedes albopictus (Diptera: Culicidae)","volume":"49","author":"Jackson","year":"2012"},{"key":"ref41","series-title":"Identification Guide to the Mosquitoes of Connecticut","author":"Andreadis","year":"2005"},{"key":"ref42","article-title":"Effect of Differential Nutrition of Larvae on Adult Fitness of Aedes triseriatus","author":"McCombs","year":"1980"},{"key":"ref43","series-title":"Am J Trop Med Hyg","first-page":"1154","article-title":"The effect of laboratory colonization on the vector-pathogen interactions of Egyptian Culex pipiens and Rift Valley fever virus","volume":"32","author":"Gargan","year":"1983"},{"key":"ref44","series-title":"J Med Entomol","first-page":"1008","article-title":"Effect of the anesthetizing agent triethylamine on western equine encephalomyelitis and St. Louis encephalitis viral titers in mosquitoes (Diptera, Culicidae)","volume":"27","author":"Kramer","year":"1990"},{"key":"ref45","series-title":"Mosq News","first-page":"130","article-title":"An in vitro feeding technique for artificially demonstrating virus transmission by mosquitoes","volume":"37","author":"Aitken","year":"1977"},{"key":"ref46","series-title":"J Am Mosq Control Assoc","first-page":"378","article-title":"Variation in the vector competence of geographic strains of Aedes albopictus for dengue 1 virus","volume":"3","author":"Boromisa","year":"1987"},{"key":"ref47","series-title":"Vector Borne Zoonotic Dis","first-page":"1493","article-title":"Larval nutritional stress does not affect vector competence for West Nile virus (WNV) in Culex tarsalis","volume":"11","author":"Dodson","year":"2011"},{"key":"ref48","series-title":"Am Stat","first-page":"119","article-title":"Approximate is better than \u201cexact\u201d for interval estimation of binomial proportions","volume":"52","author":"Agresti","year":"1998"},{"key":"ref49","series-title":"Pooled Infection Rate","author":"Biggerstaff","year":"2006"},{"key":"ref50","series-title":"J Med Entomol","first-page":"678","article-title":"Vector competence of Culex restuans (Diptera: Culicidae) from two regions of Chicago with low and high prevalence of West Nile virus human infections","volume":"49","author":"Mutebi","year":"2012"},{"issue":"Suppl","key":"ref51","series-title":"Am J Trop Med Hyg","first-page":"374","article-title":"La Crosse encephalitis in eastern Tennessee: evidence of invasive mosquito (Aedes albopictus and Ochlerotatus japonicus) involvement in the transmission of an indigenous disease","volume":"85","author":"Westby","year":"2011"},{"key":"ref52","series-title":"Emerg Infect Dis","first-page":"646","article-title":"La Crosse virus in Aedes japonicus japonicus mosquitoes in the Appalachian Region, United States","volume":"21","author":"Harris","year":"2015"},{"key":"ref53","series-title":"Am J Trop Med Hyg","first-page":"476","article-title":"Experimental infection of chipmunks and squirrels with La Crosse and trivittatus viruses and biological transmission of La Crosse virus by Aedes triseriatus","volume":"21","author":"Pantuwatana","year":"1972"},{"key":"ref54","series-title":"J Med Entomol","first-page":"378","article-title":"Aedes triseriatus (Diptera, Culicidae) and La Crosse virus. IV. Nutritional deprivation of larvae affects the adult barriers to infection and transmission","volume":"28","author":"Grimstad","year":"1991"},{"key":"ref55","series-title":"Am J Trop Med Hyg","first-page":"688","article-title":"Size alters susceptibility of vectors to dengue virus infection and dissemination","volume":"79","author":"Alto","year":"2008"},{"key":"ref56","series-title":"Vector Borne Zoonotic Dis","first-page":"727","article-title":"Nutritional stress affects mosquito survival and vector competence for West Nile virus","volume":"8","author":"Vaidyanathan","year":"2008"},{"key":"ref57","series-title":"Trop Med Int Health","first-page":"955","article-title":"Larval environmental stress alters Aedes aegypti competence for Sindbis virus","volume":"16","author":"Muturi","year":"2011"},{"key":"ref58","series-title":"J Med Entomol","first-page":"123","article-title":"Larval diet, adult size, and susceptibility of Aedes aegypti (Diptera, Culicidae) to infection with Ross river virus","volume":"31","author":"Nasci","year":"1994"},{"key":"ref59","series-title":"Vector Borne Zoonotic Dis","first-page":"241","article-title":"Larval environmental temperature and the susceptibility of Aedes albopictus Skuse (Diptera: Culicidae) to chikungunya virus","volume":"10","author":"Westbrook","year":"2010"},{"key":"ref60","series-title":"Am J Trop Med Hyg","first-page":"283","article-title":"Effect of size and geographic origin of Aedes aegypti on oral infection with dengue-2 virus","volume":"58","author":"Sumanochitrapon","year":"1998"},{"key":"ref61","series-title":"J Med Entomol","first-page":"631","article-title":"Effects of water quality on the vector competence of Culex tarsalis (Diptera: Culicidae) for western equine encephalomyelitis (Togaviridae) and St. Louis encephalitis (Flaviviridae) viruses","volume":"34","author":"Reisen","year":"1997"},{"key":"ref62","series-title":"Ann Entomol Soc Am","first-page":"116","article-title":"Host preferences of mosquitoes (Diptera: Culicidae) in Suffolk County, New York","volume":"61","author":"Means","year":"1968"},{"key":"ref63","series-title":"Ann Entomol Soc Am","first-page":"777","article-title":"Associations of Wisconsin mosquitoes and woodland vertebrate hosts","volume":"63","author":"Wright","year":"1970"},{"key":"ref64","series-title":"Am J Trop Med Hyg","first-page":"268","article-title":"Host selection by Culex pipiens mosquitoes and West Nile Virus amplification","volume":"80","author":"Hamer","year":"2009"},{"key":"ref65","series-title":"Am J Trop Med Hyg","first-page":"667","article-title":"Genetic influences on mosquito feeding behavior and the emergence of zoonotic pathogens","volume":"77","author":"Kilpatrick","year":"2007"},{"key":"ref66","series-title":"Vector Borne Zoonotic Dis","first-page":"637","article-title":"Genetic variation associated with mammalian feeding in Culex pipiens from a West Nile virus epidemic region in Chicago, Illinois","volume":"9","author":"Huang","year":"2009"},{"key":"ref67","series-title":"J Am Mosq Control Assoc","first-page":"106","article-title":"A comparison of above-ground and below-ground populations of Culex pipiens pipiens in Chicago, Illinois, and New York city, New York, using 2 microsatellite assays","volume":"28","author":"Kothera","year":"2012"},{"key":"ref68","series-title":"Am J Trop Med Hyg","first-page":"153","article-title":"The distribution of Culex p. pipiens and C. p. quinquefasciatus in North America","volume":"6","author":"Barr","year":"1957"},{"key":"ref69","series-title":"Am J Trop Med Hyg","first-page":"434","article-title":"Reexamination of Culex pipiens hybridization zone in the eastern United States by ribosomal DNA-based single nucleotide polymorphism markers","volume":"85","author":"Huang","year":"2011"},{"key":"ref70","series-title":"Parasit Vectors","first-page":"305","article-title":"The effect of hybridization of Culex pipiens complex mosquitoes on transmission of West Nile virus","volume":"6","author":"Ciota","year":"2013"},{"key":"ref71","series-title":"J Am Mosq Control Assoc","first-page":"21","article-title":"Emergence of Culex pipiens from overwintering hibernacula","volume":"27","author":"Ciota","year":"2011"},{"key":"ref72","series-title":"Am J Trop Med Hyg","first-page":"168","article-title":"Effect of La Crosse virus infection on overwintering of Aedes triseriatus","volume":"58","author":"McGaw","year":"1998"},{"key":"ref73","series-title":"J Am Mosq Control Assoc","first-page":"89","article-title":"The association of artificial containers and La Crosse encephalitis cases in Minnesota, 1979","volume":"1","author":"Hedberg","year":"1985"},{"key":"ref74","series-title":"Am J Epidemiol","first-page":"320","article-title":"Symptomatic La Crosse virus infections of the central nervous system\u2014a study of risk factors in an endemic area","volume":"136","author":"Woodruff","year":"1992"}],"container-title":["The American 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