{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T01:21:12Z","timestamp":1790299272761,"version":"4.1.0"},"reference-count":52,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,11,5]]},"abstract":"<jats:p>St. Louis encephalitis virus (SLEV) has shown greater susceptibility to oral infectivity than West Nile virus (WNV) in <jats:italic>Culex<\/jats:italic> mosquitoes. To identify the viral genetic elements that modulate these disparate phenotypes, structural chimeras (WNV\u2013pre-membrane [prM] and envelope [E] proteins [prME]\/SLEV.IC (infectious clone) and SLEV-prME\/WNV.IC) were constructed in which two of the structural proteins, the prM and E, were interchanged between viruses. Oral dose\u2013response assessment with the chimeric\/parental WNV and SLEV was performed to characterize the infection phenotypes in <jats:italic>Culex<\/jats:italic> mosquitoes by artificial blood meals. The median infectious dose required to infect 50% of <jats:italic>Cx. quinquefasciatus<\/jats:italic> with WNV was indistinguishable from that of the SLEV-prME\/WNV.IC chimeric virus. Similarly, SLEV and WNV-prME\/SLEV.IC virus exhibited an indistinguishable oral dose\u2013response relationship in <jats:italic>Cx. quinquefasciatus<\/jats:italic>. Infection rates for WNV.IC and SLEV-prME\/WNV.IC were significantly lower than SLEV.IC and WNV-prME\/SLEV.IC infection rates. These results indicated that WNV and SLEV oral infectivities are not mediated by genetic differences within the prM and E proteins.<\/jats:p>","DOI":"10.4269\/ajtmh.14-0289","type":"journal-article","created":{"date-parts":[[2014,8,26]],"date-time":"2014-08-26T03:11:26Z","timestamp":1409022686000},"page":"1066-1072","source":"Crossref","is-referenced-by-count":9,"title":["Genetic Determinants of Differential Oral Infection Phenotypes of West Nile and St. Louis Encephalitis Viruses in Culex spp. Mosquitoes"],"prefix":"10.4269","volume":"91","author":[{"given":"Payal D.","family":"Maharaj","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bethany G.","family":"Bolling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Anishchenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William 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