{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:36:41Z","timestamp":1791344201657,"version":"4.3.1"},"reference-count":16,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,3]]},"abstract":"<jats:p>In malaria-endemic regions, many medical facilities have limited capacity to diagnose non-malarial etiologies of acute febrile illness (AFI). As a result, the etiology of AFI is seldom determined, although AFI remains a major cause of morbidity in developing countries. An outbreak of AFI was reported in the Afar region of Ethiopia in August of 2011. Retrospectively, 12,816 suspected AFI cases were identified by review of medical records. Symptoms were mild and self-limiting within 3 days after the date of onset; no fatalities were identified. All initial test results of AFI patient specimens were negative for selected pathogens using standard microbiological and molecular techniques. High-throughput sequencing of nucleic acid extracts of serum specimens from 29 AFI cases identified 17 (59%) of 29 samples as positive for Sandfly Fever Sicilian Virus (SFSV). These results were further confirmed by specific reverse transcription polymerase chain reaction. This is the first study implicating SFSV as an etiological agent for AFI in Ethiopia.<\/jats:p>","DOI":"10.4269\/ajtmh.14-0299","type":"journal-article","created":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T01:21:41Z","timestamp":1412040101000},"page":"1250-1253","source":"Crossref","is-referenced-by-count":29,"title":["An Outbreak of Acute Febrile Illness Caused by Sandfly Fever Sicilian Virus in the Afar Region of Ethiopia, 2011"],"prefix":"10.4269","volume":"91","author":[{"given":"Abyot Bekele","family":"Woyessa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Omballa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Lambert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilian","family":"Waiboci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Workenesh","family":"Ayele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdi","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Negga Asamene","family":"Abera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melvin","family":"Ochieng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel M.","family":"Montgomery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daddi","family":"Jima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"Fields","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Emerg Infect Dis","first-page":"302","article-title":"Clinical microbiology in developing countries","volume":"7","author":"Archibald","year":"2001"},{"key":"R2","series-title":"Emerg Inf Dis","first-page":"539","article-title":"Estimating the incidence of typhoid fever and other febrile illnesses in developing countries","volume":"9","author":"Crump","year":"2003"},{"key":"R3","series-title":"Jpn J Infect Dis","first-page":"107","article-title":"Febrile illnesses of different etiology among outpatients in four health centers in northwestern Ethiopia","volume":"62","author":"Animut","year":"2009"},{"key":"R4","series-title":"Euro Surveill","first-page":"19507","article-title":"Arthropod- borne viruses transmitted by Phlebotomine sandflies in Europe: a review","volume":"15","author":"Depaquit","year":"2010"},{"key":"R5","series-title":"Curr Opin Infect Dis","first-page":"383","article-title":"Epidemiological, clinical and laboratory aspects of sandfly fever","volume":"16","author":"Dionisio","year":"2003"},{"key":"R6","series-title":"Bull World Health Organ","first-page":"663","article-title":"Serological studies on the epidemiology of sandfly fever in the old world","volume":"54","author":"Tesh","year":"1976"},{"key":"R7","series-title":"J Clin Microbiol","first-page":"2323","article-title":"Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR","volume":"40","author":"Drosten","year":"2002"},{"key":"R8","series-title":"Cell","first-page":"253","article-title":"Pathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome","volume":"151","author":"Handley","year":"2012"},{"key":"R9","series-title":"PLoS ONE","first-page":"e78470","article-title":"Identification of novel viruses using VirusHunter\u2013an automated data analysis pipeline","volume":"8","author":"Zhao","year":"2013"},{"key":"R10","series-title":"J Clin Microbiol","first-page":"2398","article-title":"Consensus amplification and novel multiplex sequencing method for s segment species identification of 47 viruses of the Orthobunyavirus, Phlebovirus, and Nairovirus genera of the family Bunyaviridae","volume":"47","author":"Lambert","year":"2009"},{"key":"R11","series-title":"Nucleic Acids Res","first-page":"4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994"},{"key":"R12","series-title":"Mol Biol Evol","first-page":"1596","article-title":"MEGA4: molecular Evolutionary Genetics Analysis (MEGA) software version 4.0","volume":"24","author":"Tamura","year":"2007"},{"key":"R13","series-title":"Mol Biol Evol","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987"},{"key":"R14","series-title":"Int J Infect Dis","first-page":"244","article-title":"A sandfly fever virus outbreak in the east Mediterranean region of Turkey","volume":"16","author":"Guler","year":"2012"},{"key":"R15","series-title":"Open Virol J","first-page":"15","article-title":"Molecular and serological evidence for the presence of novel phleboviruses in sandflies from northern Algeria","volume":"4","author":"Moureau","year":"2010"},{"key":"R16","series-title":"J Gen Virol","first-page":"1275","article-title":"Punique virus, a novel phlebovirus, related to sandfly fever Naples virus, isolated from sandflies collected in Tunisia","volume":"91","author":"Zhioua","year":"2010"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/91\/6\/article-p1250.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/91\/6\/article-p1250.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/91\/6\/article-p1250.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T21:03:27Z","timestamp":1723064607000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/91\/6\/article-p1250.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,3]]},"references-count":16,"journal-issue":{"issue":"6"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.14-0299","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,3]]}}}