{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T14:40:38Z","timestamp":1784299238382,"version":"3.55.0"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,3,4]]},"abstract":"<jats:p>Cutaneous leishmaniasis (CL) has been introduced to the <jats:italic>Leishmania<\/jats:italic> under-endemic Lebanese population in an uncontrolled manner as a result of recent large-scale displacement of refugees from endemic Syria. Accordingly, a quick and reliable method to diagnose CL is essential. Matched punch biopsies and air-dried scrapings on 72 patients were obtained. Scrapings were collected in two forms: thick drop (<jats:italic>N<\/jats:italic> = 33) or thin smear (<jats:italic>N<\/jats:italic> = 39). Clinical information was recorded. Sections of punch biopsies and scrapings were stained and examined microscopically. Polymerase chain reaction (PCR) was performed on both scraping forms and biopsies. The diagnostic sensitivity of the tests performed revealed that microscopy in conjunction with PCR on punch biopsies was the most sensitive test (93%) overall. However, taken individually, microscopy and PCR yielded the highest sensitivities when performed on drop scrapings (63% and 85%, respectively), and not smear scrapings (38% and 56%, respectively) as compared with the punch biopsies (44% and 83%, respectively). Microscopic concordance for punch biopsies and drop scrapings was present in 25 of 33 cases. Concordance was predicted only by the high\/low parasitic index (PI: 3.1 \u00b1 1.7 and 0.4 \u00b1 0.5, respectively; <jats:italic>P<\/jats:italic> &lt; 0.05). Herein, we optimized a novel rapid method for reliable diagnosis of CL based on drop scrapings with good agreement with the gold standard punch biopsy technique.<\/jats:p>","DOI":"10.4269\/ajtmh.14-0512","type":"journal-article","created":{"date-parts":[[2015,1,6]],"date-time":"2015-01-06T02:19:08Z","timestamp":1420510748000},"page":"518-522","source":"Crossref","is-referenced-by-count":29,"title":["Diagnosis of Cutaneous Leishmaniasis: Why Punch When You Can Scrape?"],"prefix":"10.4269","volume":"92","author":[{"given":"Mario","family":"Saab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hussein","family":"El Hage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalil","family":"Charafeddine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Habib","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"Khalifeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"PLoS ONE","first-page":"e35671","article-title":"WHO Leishmaniasis Control Team 2012. 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Epidemiology and public health significance of leishmaniasis","volume":"4","author":"Dawit","year":"2013"},{"key":"R14","series-title":"Braz J Med Biol","first-page":"421","article-title":"Comparison of the specificity of PCR and the histopathological detection of Leishmania for the diagnosis of American cutaneous leishmaniasis","volume":"35","author":"Medeiros","year":"2002"},{"key":"R15","series-title":"Diagn Microbiol Infect Dis","first-page":"349","article-title":"PCR diagnosis and characterization of Leishmania in local and imported clinical samples","volume":"47","author":"Sch\u00f6nian","year":"2003"},{"key":"R16","series-title":"Acta Trop","first-page":"55","article-title":"Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of Giemsa-stained smears","volume":"99","author":"Al-Jawabreh","year":"2006"},{"key":"R17","series-title":"Iran J Parasitol","first-page":"382","article-title":"Identification of Leishmania species using PCR assay on Giemsa-stained slides prepared from cutaneous leishmaniasis patients","volume":"8","author":"Kheirandish","year":"2013"},{"key":"R18","series-title":"J Trop Med Hyg","first-page":"807","article-title":"An epidemic outbreak of canine cutaneous leishmaniasis in Colombia caused by Leishmania braziliensis and Leishmania panamensis","volume":"86","author":"V\u00e9lez","year":"2012"},{"key":"R19","series-title":"T Roy Soc Trop Med H","first-page":"133","article-title":"An outbreak of cutaneous leishmaniasis in an Afghan refugee settlement in north-west Pakistan","volume":"93","author":"Rowland","year":"1999"},{"key":"R20","series-title":"Mem Inst Oswaldo Cruz","first-page":"119","article-title":"Report of an epidemic outbreak of tegumentary leishmaniasis in a coffee-growing area of Colombia","volume":"85","author":"Montoya","year":"1990"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/92\/3\/article-p518.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/92\/3\/article-p518.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/92\/3\/article-p518.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T19:27:54Z","timestamp":1723058874000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/92\/3\/article-p518.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,4]]},"references-count":20,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.14-0512","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,4]]}}}