{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T10:15:54Z","timestamp":1783764954572,"version":"3.55.0"},"reference-count":22,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,4,1]]},"abstract":"<jats:p>The use of primaquine and other 8-aminoquinolines for malaria elimination is hampered by, among other factors, the limited availability of point-of-care tests for the diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency. Historically, the most used source of blood for G6PD analyses is venous blood, whereas diagnostic devices used in the field require the use of capillary blood; data have shown that the two sources of blood often differ with respect to hemoglobin concentration and number of red blood cells. Therefore, we have analyzed, in both capillary and venous blood drawn from the same healthy donors, the correlation of G6PD activity assessed by two qualitative tests (the Fluorescent Spot test and the CareStart test) with the gold standard quantitative spectrophotometric assay. Results obtained on 150 subjects with normal, intermediate, and deficient G6PD phenotypes show that, although differences exist between the aforementioned characteristics in capillary and venous blood, these do not impact on the quantitative assessment of G6PD activity after corrected for hemoglobin concentration or red blood cell count. Furthermore, we have assessed the sensitivity and specificity of the two qualitative tests against the gold standard spectrophotometric assay at different activity thresholds of residual enzymatic activity in both blood sources.<\/jats:p>","DOI":"10.4269\/ajtmh.14-0696","type":"journal-article","created":{"date-parts":[[2015,2,3]],"date-time":"2015-02-03T02:58:31Z","timestamp":1422932311000},"page":"818-824","source":"Crossref","is-referenced-by-count":34,"title":["Suitability of Capillary Blood for Quantitative Assessment of G6PD Activity and Performances of G6PD Point-of-Care Tests"],"prefix":"10.4269","volume":"92","author":[{"given":"Germana","family":"Bancone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy S.","family":"Chu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nongnud","family":"Chowwiwat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raweewan","family":"Somsakchaicharoen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pornpimon","family":"Wilaisrisak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakaykaew","family":"Charunwatthana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Bansil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"McGray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo J.","family":"Domingo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois H.","family":"Nosten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Malar J","first-page":"418","article-title":"Spatial distribution of G6PD deficiency variants across malaria-endemic regions","volume":"12","author":"Howes","year":"2013"},{"key":"R2","series-title":"J Hum Genet","first-page":"48","article-title":"Glucose-6-phosphate dehydrogenase mutations in Mon and Burmese of southern Myanmar","volume":"53","author":"Nuchprayoon","year":"2008"},{"key":"R3","series-title":"Malar J","first-page":"368","article-title":"Prevalence and distribution of glucose-6-phosphate dehydrogenase (G6PD) variants in Thai and Burmese populations in malaria endemic areas of 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in the detection of glucose-6-phosphate dehydrogenase deficiency","volume":"12","author":"Nantakomol","year":"2013"},{"key":"R16","series-title":"Southeast Asian J Trop Med Public Health","first-page":"405","article-title":"Semiquantitative screening test for G6PD deficiency detects severe deficiency but misses a substantial proportion of partially-deficient females","volume":"34","author":"Ainoon","year":"2003"},{"key":"R17","series-title":"Int J Lab Hematol","first-page":"463","article-title":"Modification to reporting of qualitative fluorescent spot test results improves detection of glucose-6-phosphate dehydrogenase (G6PD)-deficient heterozygote female newborns","volume":"33","author":"Nadarajan","year":"2011"},{"key":"R18","series-title":"Am J Trop Med Hyg","first-page":"854","article-title":"Comparison of quantitative and qualitative tests for glucose-6-phosphate dehydrogenase deficiency","volume":"91","author":"LaRue","year":"2014"},{"key":"R19","series-title":"Lancet Infect 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