{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T06:50:57Z","timestamp":1787986257157,"version":"build-2784847793"},"reference-count":33,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,7,8]]},"abstract":"<jats:p>The mosquito <jats:italic>Aedes aegypti<\/jats:italic> is a vector of yellow fever, dengue, and chikungunya. Control of the insect is crucial to stop the spread of dengue and chikungunya, so it is critically important to understand its mating behavior. Primarily, based on laboratory behavior, it has long been assumed that <jats:italic>Ae. aegypti<\/jats:italic> females mate once in their lifetime. However, multiple inseminations have been observed in semi-field and laboratory settings, and in closely related species. Here, we report the first evidence of polyandry in a natural population of <jats:italic>Ae. aegypti<\/jats:italic>. Female <jats:italic>Ae. aegypti<\/jats:italic> were captured around the New Orleans, LA, metropolitan area. They were offered a blood meal and allowed to lay eggs, which were reared to the third-instar larval stage. A parentage analysis using four microsatellite loci was performed. Out of 48 families, 3 showed evidence of multiple paternity. An expanded analysis of these three families found that one family group included offspring contributed by three fathers, and the other two included offspring from two fathers. This result establishes that polyandry can occur in a small proportion of <jats:italic>Ae. aegypti<\/jats:italic> females in a natural setting. This could complicate future genetic control efforts and has implications for sampling for population genetics.<\/jats:p>","DOI":"10.4269\/ajtmh.14-0718","type":"journal-article","created":{"date-parts":[[2015,4,14]],"date-time":"2015-04-14T01:46:10Z","timestamp":1428975970000},"page":"189-193","source":"Crossref","is-referenced-by-count":36,"title":["Evidence of Limited Polyandry in a Natural Population of Aedes aegypti"],"prefix":"10.4269","volume":"93","author":[{"given":"Joshua B.","family":"Richardson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel B.","family":"Jameson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Gloria-Soria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawn M.","family":"Wesson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Powell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Vector Borne Zoonotic Dis","first-page":"18","article-title":"Sterile-insect methods for control of mosquito-borne diseases: an analysis","volume":"10","author":"Alphey","year":"2010"},{"key":"R2","series-title":"J Vis Exp","first-page":"3579","article-title":"Mass production of genetically modified Aedes aegypti for field releases in Brazil","volume":"83","author":"Carvalho","year":"2014"},{"key":"R3","series-title":"Nat Biotechnol","first-page":"1034","article-title":"Field performance of engineered male mosquitoes","volume":"29","author":"Harris","year":"2011"},{"key":"R4","series-title":"Anim Behav","first-page":"145","article-title":"The evolution of polyandry: multiple mating and female fitness in insects","volume":"60","author":"Arnqvist","year":"2000"},{"key":"R5","series-title":"Annu Rev Entomol","first-page":"413","article-title":"Mating systems of blood-feeding flies","volume":"51","author":"Yuval","year":"2006"},{"key":"R6","series-title":"J Insect Physiol","first-page":"5","article-title":"Internal regulation of rate of digestion of blood meals in the mosquito, Aedes aegypti","volume":"21","author":"Downe","year":"1975"},{"key":"R7","series-title":"Can Entomol","first-page":"6","article-title":"Functions of the male accessory gland secretions of Aedes mosquitoes (Diptera: Culicidae): transplantation studies","volume":"108","author":"Ramalingam","year":"1976"},{"key":"R8","series-title":"Biol Bull","first-page":"201","article-title":"Female sexual behavior as the mechanism rendering Aedes aegypti refractory to insemination","volume":"140","author":"Gwadz","year":"1971"},{"key":"R9","series-title":"Science","first-page":"1499","article-title":"Mosquitoes: female monogamy induced by male accessory gland substance","volume":"156","author":"Craig","year":"1967"},{"key":"R10","series-title":"Insect Biochem Mol Biol","first-page":"176","article-title":"Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: potential tools for control of female feeding and reproduction","volume":"38","author":"Sirot","year":"2008"},{"key":"R11","series-title":"J Insect Physiol","first-page":"931","article-title":"The partial purification and separation of the protein components of matrone from Aedes aegypti","volume":"16","author":"Fuchs","year":"1970"},{"key":"R12","series-title":"Life Sci","first-page":"835","article-title":"The biochemical basis of female monogamy in mosquitoes. 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