{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:05:05Z","timestamp":1791266705019,"version":"4.1.0"},"reference-count":53,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,11,4]]},"abstract":"\n This study was conducted from September 2011 to March 2014 to address the occurrence and genotypes of\n Coxiella burnetii<\/jats:italic>\n using molecular methods in ticks collected from domestic animals in Ethiopia. Ticks were tested for\n C. burnetii<\/jats:italic>\n by quantitative real-time polymerase chain reaction (qPCR) targeting two different genes followed by multispacer sequence typing (MST). An overall prevalence of 6.4% (54\/842) of\n C. burnetii<\/jats:italic>\n was recorded.\n C. burnetii<\/jats:italic>\n was detected in 28.6% (14\/49) of\n Amblyomma gemma<\/jats:italic>\n , 25% (31\/124) of\n Rhipicephalus pulchellus<\/jats:italic>\n , 7.1% (1\/14) of\n Hyalomma marginatum rufipes<\/jats:italic>\n , 3.2% (2\/62) of\n Am. variegatum,<\/jats:italic>\n 3.1% (4\/128) of\n Am. cohaerens<\/jats:italic>\n , 1.6% (1\/63) of\n Rh. praetextatus,<\/jats:italic>\n and 0.6% (1\/153) of\n Rhipicephalus (Boophilus) decoloratus<\/jats:italic>\n . Significantly higher overall frequencies of\n C. burnetii<\/jats:italic>\n DNA were observed in\n Am. gemma<\/jats:italic>\n and\n Rh. pulchellus<\/jats:italic>\n than in other tick species (Mantel\u2013Haenszel [MH],\n P<\/jats:italic>\n < 0.0001). The overall frequency of\n C. burnetii<\/jats:italic>\n was significantly higher (MH,\n P<\/jats:italic>\n < 0.0001) in ticks from southeastern districts (Arero, Moyale, and Yabelo) than that from other districts. This study demonstrated the presence of\n C. burnetii<\/jats:italic>\n genotype MST 18 in ticks in southeastern districts and genotype MST 20 in ticks in central districts. This study highlights the importance of ticks in the epidemiology of\n C. burnetii<\/jats:italic>\n in Ethiopia.\n <\/jats:p>","DOI":"10.4269\/ajtmh.14-0758","type":"journal-article","created":{"date-parts":[[2015,9,21]],"date-time":"2015-09-21T23:25:16Z","timestamp":1442877916000},"page":"1074-1081","source":"Crossref","is-referenced-by-count":50,"title":["Occurrence and Genotyping of Coxiella burnetii in Ixodid Ticks in Oromia, Ethiopia"],"prefix":"10.4269","volume":"93","author":[{"given":"Bersissa","family":"Kumsa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Socolovschi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Almeras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Parola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"issue":"(Suppl)","key":"R1","series-title":"Parasitology","first-page":"S3","article-title":"The global importance of ticks","volume":"129","author":"Jongejan","year":"2004"},{"key":"R2","series-title":"Trends Parasitol","first-page":"437","article-title":"Ticks and tick-borne diseases: a One Health perspective","volume":"28","author":"Dantas-Torres","year":"2012"},{"key":"R3","series-title":"Ticks Tick Borne Dis","first-page":"115","article-title":"Ticks and tick-borne pathogens on the rise","volume":"3","author":"De la Fuente","year":"2012"},{"key":"R4","series-title":"Onderstepoort J Vet Res","first-page":"41","article-title":"Tick infestation of Borana cattle in the Borana Province of Ethiopia","volume":"68","author":"Regassa","year":"2001"},{"key":"R5","series-title":"Ticks Tick Borne Dis","first-page":"8","article-title":"Spotted fever group rickettsiae in ixodid ticks in Oromia, Ethiopia","volume":"6","author":"Kumsa","year":"2015"},{"key":"R6","series-title":"Vet Microbiol","first-page":"297","article-title":"Q fever","volume":"140","author":"Angelakis","year":"2010"},{"key":"R7","series-title":"Clin Microbiol Rev","first-page":"518","article-title":"Q fever","volume":"12","author":"Maurin","year":"1999"},{"key":"R8","series-title":"Euro Surveill","article-title":"Q fever in humans and farm animals in four European countries, 1982 to 2010","volume":"18","author":"Georgiev","year":"2013"},{"key":"R9","series-title":"J Infect","first-page":"313","article-title":"Q fever","volume":"54","author":"Cutler","year":"2007"},{"key":"R10","series-title":"Vet Microbiol","first-page":"1","article-title":"Prevalence of Coxiella burnetii infection in domestic ruminants: a critical review","volume":"149","author":"Guatteo","year":"2011"},{"key":"R11","series-title":"Acta Vet Scand","first-page":"13","article-title":"Coxiella burnetii associated reproductive disorders in domestic animals\u2013a critical review","volume":"55","author":"Agerholm","year":"2013"},{"key":"R12","series-title":"PLoS Negl Trop Dis","first-page":"e654","article-title":"Coxiella burnetii in humans and ticks in rural Senegal","volume":"4","author":"Mediannikov","year":"2010"},{"key":"R13","series-title":"Vector Borne Zoonotic Dis","first-page":"685","article-title":"Presence of Coxiella burnetii in fleas in Cyprus","volume":"14","author":"Psaroulaki","year":"2014"},{"key":"R14","series-title":"Am J Trop Med Hyg","first-page":"41","article-title":"Surveillance of Egyptian fleas for agents of public health significance: Anaplasma, Bartonella, Coxiella, Ehrlichia, Rickettsia, and Yersinia pestis","volume":"75","author":"Loftis","year":"2006"},{"key":"R15","series-title":"Emerg Infect Dis","first-page":"1002","article-title":"Coxiella burnetii in wild-caught filth flies","volume":"14","author":"Nelder","year":"2008"},{"key":"R16","series-title":"PLoS Negl Trop Dis","first-page":"e2787","article-title":"Epidemiology of Coxiella burnetii infection in Africa: a One Health systematic review","volume":"8","author":"Vanderburg","year":"2014"},{"key":"R17","series-title":"Clin Infect Dis","first-page":"1126","article-title":"Prevalence of antibodies to Coxiella burnetti, Rickettsia conorii, and Rickettsia typhi in seven African countries","volume":"21","author":"Dupont","year":"1995"},{"key":"R18","series-title":"Am J Trop Med Hyg","first-page":"513","article-title":"Coxiella burnetii in humans, domestic ruminants, and ticks in rural western Kenya","volume":"88","author":"Knobel","year":"2013"},{"key":"R19","series-title":"PLoS ONE","first-page":"e85424","article-title":"Coxiella burnetii seroprevalence in small ruminants in the Gambia","volume":"9","author":"Klaasen","year":"2014"},{"key":"R20","series-title":"Am J Trop Med Hyg","first-page":"518","article-title":"First genetic detection of Coxiella burnetii in Zambian livestock","volume":"89","author":"Qiu","year":"2013"},{"key":"R21","series-title":"Int J Infect Dis","first-page":"107","article-title":"Coxiella burnetii-positive PCR in febrile patients in rural and urban Africa","volume":"28","author":"Angelakis","year":"2014"},{"key":"R22","series-title":"Bull World Health Organ","first-page":"127","article-title":"Evidence of rickettsial disease agents in ticks from Ethiopian cattle","volume":"35","author":"Philip","year":"1966"},{"key":"R23","series-title":"J Vet Sci Med Diagn","first-page":"1","article-title":"Seroprevalence of brucellosis and Q-fever in southeast Ethiopian pastoral livestock","volume":"2","author":"Gumi","year":"2013"},{"key":"R24","series-title":"Emerg Infect Dis","first-page":"1211","article-title":"Coxiella burnetii genotyping","volume":"11","author":"Glazunova","year":"2005"},{"key":"R25","series-title":"BMC Vet Res","first-page":"241","article-title":"Genotyping of Coxiella burnetii from domestic ruminants in northern Spain","volume":"8","author":"Astobiza","year":"2012"},{"key":"R26","series-title":"BMC Vet Res","first-page":"107","article-title":"Genotyping of Coxiella burnetii from domestic ruminants and human in Hungary: 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