{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:57:06Z","timestamp":1774659426160,"version":"3.50.1"},"reference-count":16,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,5,4]]},"abstract":"<jats:p>Rodents play an important role in the transmission of pathogenic <jats:italic>Leptospira<\/jats:italic> spp. However, in Angola, neither the natural reservoirs of these spirochetes nor leptospirosis diagnosis has been considered. Regarding this gap, we captured rodents in Luanda and Huambo provinces to identify circulating <jats:italic>Leptospira<\/jats:italic> spp. Rodent kidney tissue was cultured and DNA amplified and sequenced. Culture isolates were evaluated for pathogenic status and typing with rabbit antisera; polymerase chain reaction (PCR) and sequencing were also performed. A total of 37 rodents were captured: <jats:italic>Rattus rattus<\/jats:italic> (15, 40.5%), <jats:italic>Rattus norvegicus<\/jats:italic> (9, 24.3%), and <jats:italic>Mus musculus<\/jats:italic> (13, 35.2%). Leptospiral DNA was amplified in eight (21.6%) kidney samples. From the cultures, we obtained four (10.8%) <jats:italic>Leptospira<\/jats:italic> isolates belonging to the Icterohaemorrhagiae and Ballum serogroups of <jats:italic>Leptospira interrogans<\/jats:italic> and <jats:italic>Leptospira borgpetersenii<\/jats:italic> genospecies, respectively. This study provides information about circulating leptospires spread by rats and mice in Angola.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0027","type":"journal-article","created":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T03:34:11Z","timestamp":1456803251000},"page":"955-958","source":"Crossref","is-referenced-by-count":5,"title":["First Isolates of Leptospira spp., from Rodents Captured in Angola"],"prefix":"10.4269","volume":"94","author":[{"given":"Elsa","family":"Fortes-Gabriel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Carreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Lu\u00edsa","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Report of the Second Meeting of the Leptospirosis Burden Epidemiology Reference Group","author":"WHO","year":"2011"},{"key":"ref2","series-title":"Eur J Epidemiol","first-page":"1151","article-title":"Rodents and Leptospira transmission risk in Terceira island (Azores)","volume":"16","author":"Collares-Pereira","year":"2000"},{"key":"ref3","series-title":"Leptospira and Leptospirosis","first-page":"272","author":"Faine","year":"1999"},{"key":"ref4","series-title":"Eur J Epidemiol","first-page":"435","article-title":"First epidemiological data on pathogenic leptospires isolated on the Azorean islands","volume":"13","author":"Collares-Pereira","year":"1997"},{"key":"ref5","series-title":"Int J Infect Dis","first-page":"47","article-title":"Leptospirosis in sub-Saharan Africa: a systematic review","volume":"28c","author":"De Vries","year":"2014"},{"key":"ref6","series-title":"Protocols for Field and Laboratory Rodent Studies","first-page":"20","author":"Herbreteau","year":"2011"},{"key":"ref7","series-title":"J Bacteriol","first-page":"1618","article-title":"Differentiation of pathogenic and saprophytic Leptospires with 8-azaguanine","volume":"88","author":"Johnson","year":"1964"},{"key":"ref8","series-title":"J Bacteriol","first-page":"27","article-title":"Differentiation of pathogenic and saprophytic leptospires. I. Growth at low temperatures","volume":"94","author":"Johnson","year":"1967"},{"key":"ref9","series-title":"FEMS Microbiol Lett","first-page":"437","article-title":"Polymerase chain reaction assay specific for pathogenic Leptospira based on the gene hap1 encoding the hemolysis-associated protein-1","volume":"243","author":"Branger","year":"2005"},{"key":"ref10","series-title":"J Clin Microbiol","first-page":"191","article-title":"Identification of new repetitive element in Leptospira interrogans serovar Copenhageni and its application to PCR-based differentiation of Leptospira serogroups","volume":"39","author":"Barocchi","year":"2001"},{"key":"ref11","series-title":"Mol Biol Evol","first-page":"2725","article-title":"MEGA6: Molecular Evolutionary Genetics Analysis version 6.0","volume":"30","author":"Tamura","year":"2013"},{"key":"ref12","series-title":"Acta Trop","first-page":"218","article-title":"A model of leptospirosis infection in an African rodent to determine risk to humans: seasonal fluctuations and the impact of rodent control","volume":"99","author":"Holt","year":"2006"},{"key":"ref13","series-title":"Zoonoses Public Health","first-page":"113","article-title":"Leptospira interrogans at the human-wildlife interface in northern Botswana: a newly identified public health threat","volume":"61","author":"Jobbins","year":"2013"},{"key":"ref14","series-title":"Am J Trop Med Hyg","first-page":"1095","article-title":"Urban leptospirosis in Africa: a cross-sectional survey of Leptospira infection in rodents in the Kibera urban settlement, Nairobi, Kenya","volume":"89","author":"Halliday","year":"2013"},{"key":"ref15","series-title":"ISRN Epidemiol","first-page":"1","article-title":"Leptospira spp. prevalence in small mammal populations in Cotonou, Benin","volume":"2013","author":"Houemenou","year":"2013"},{"key":"ref16","series-title":"J Bacteriol","first-page":"2818","article-title":"Molecular evolution and mosaicism of leptospiral outer membrane proteins involves horizontal DNA transfer","volume":"186","author":"Haake","year":"2004"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/94\/5\/article-p955.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/94\/5\/article-p955.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/94\/5\/article-p955.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T22:06:18Z","timestamp":1723068378000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/94\/5\/article-p955.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,4]]},"references-count":16,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.15-0027","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,4]]}}}