{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T10:19:42Z","timestamp":1772619582489,"version":"3.50.1"},"reference-count":21,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,6,3]]},"abstract":"<jats:p><jats:italic>Giardia lamblia<\/jats:italic> and <jats:italic>Cryptosporidium<\/jats:italic> species belong to a complex group of pathogens that cause diseases hampering development and socioeconomic improvements in the developing countries. Both pathogens are recognized as significant causes of diarrhea and nutritional disorders. However, further studies are needed to clarify the role of parasitic infections, especially asymptomatic infections in malnutrition and stunting. We developed a high-throughput multiplex quantitative polymerase chain reaction (qPCR) method for <jats:italic>G. lamblia<\/jats:italic> and <jats:italic>Cryptosporidium<\/jats:italic> spp. detection in stool samples. The sensitivity and specificity of the method were ensured by analyzing confirmed positive samples acquired from diagnostics laboratories and participating in an external quality control round. Its capability to detect asymptomatic <jats:italic>G. lamblia<\/jats:italic> and <jats:italic>Cryptosporidium<\/jats:italic> spp. infections was confirmed by analyzing stool samples collected from 44 asymptomatic 6-month-old infants living in an endemic region in Malawi. Of these, five samples were found to be positive for <jats:italic>G. lamblia<\/jats:italic> and two for <jats:italic>Cryptosporidium<\/jats:italic> spp. In conclusion, the developed method is suitable for large-scale studies evaluating the occurrence of <jats:italic>G. lamblia<\/jats:italic> and <jats:italic>Cryptosporidium<\/jats:italic> spp. in endemic regions and for clinical diagnostics of these infections.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0054","type":"journal-article","created":{"date-parts":[[2015,4,28]],"date-time":"2015-04-28T02:21:46Z","timestamp":1430187706000},"page":"1222-1226","source":"Crossref","is-referenced-by-count":10,"title":["High-Throughput Multiplex Quantitative Polymerase Chain Reaction Method for Giardia lamblia and Cryptosporidium Species Detection in Stool Samples"],"prefix":"10.4269","volume":"92","author":[{"given":"Noora","family":"Nurminen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"Juuti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sami","family":"Oikarinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-Mei","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirsi-Maarit","family":"Lehto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Mangani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Maleta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per","family":"Ashorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heikki","family":"Hy\u00f6ty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Trends 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18-month-old infants and children in rural Malawi","volume":"25","author":"Mangani","year":"2013"},{"key":"R18","series-title":"Vet Med (Praha)","first-page":"224","article-title":"Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR","volume":"57","author":"Adamska","year":"2012"},{"key":"R19","series-title":"Clin Microbiol Infect","first-page":"869","article-title":"Parasitological diagnosis combining an internally controlled real-time PCR assay for the detection of four protozoa in stool samples with a testing algorithm for microscopy","volume":"15","author":"Bruijnesteijn van Coppenraet","year":"2009"},{"key":"R20","series-title":"J Clin Microbiol","first-page":"2556","article-title":"Multicentric evaluation of a new real-time PCR assay for quantification of Cryptosporidium spp. and identification of Cryptosporidium parvum and Cryptosporidium hominis","volume":"51","author":"Mary","year":"2013"},{"key":"R21","series-title":"Clin 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