{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:23:15Z","timestamp":1790947395121,"version":"4.1.0"},"reference-count":23,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,9,2]]},"abstract":"<jats:p>Melioidosis is a severe disease caused by the Gram-negative bacterium <jats:italic>Burkholderia pseudomallei<\/jats:italic>. Diagnosis of melioidosis currently relies on the isolation of <jats:italic>B. pseudomallei<\/jats:italic> from clinical samples, which can take several days. An indirect hemagglutination assay (IHA) is widely used for serodiagnosis, but it has a short shelf life, is poorly standardized, and requires a viable bacteria culture performed in a biosafety level 3 (BSL-3) laboratory. To improve the diagnostic methods, we have developed two rapid latex agglutination tests based on purified <jats:italic>B. pseudomallei<\/jats:italic> O-polysaccharide (OPS) and capsular polysaccharide (CPS) antigens. The immunodiagnostic potential of these tests was evaluated using serum from culture-confirmed melioidosis patients (<jats:italic>N<\/jats:italic> = 143) and healthy donors from either endemic (<jats:italic>N<\/jats:italic> = 199) or non-endemic areas (<jats:italic>N<\/jats:italic> = 90). The sensitivity of the OPS-based latex agglutination assay (OPS-latex; 84.4%) was significantly higher than both the CPS-latex (69.5%) (<jats:italic>P<\/jats:italic> &lt; 0.001) and IHA (69.5%) (<jats:italic>P<\/jats:italic> = 0.001). When evaluated with Thai donor serum, the OPS-latex had comparable specificity (56.9%) to the CPS-latex (63.8%) (<jats:italic>P<\/jats:italic> = 0.053), but was significantly lower than the IHA (67.6%) (<jats:italic>P<\/jats:italic> = 0.002). In contrast, all tests with U.S. donor serum were highly specific (\u2265 97.8%). These results suggest that polysaccharide-based latex agglutination assays may be useful for serodiagnosis of melioidosis in non-endemic areas.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0114","type":"journal-article","created":{"date-parts":[[2015,6,30]],"date-time":"2015-06-30T03:05:06Z","timestamp":1435633506000},"page":"542-546","source":"Crossref","is-referenced-by-count":35,"title":["Evaluation of Polysaccharide-Based Latex Agglutination Assays for the Rapid Detection of Antibodies to Burkholderia pseudomallei"],"prefix":"10.4269","volume":"93","author":[{"given":"Vichaya","family":"Suttisunhakul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narisara","family":"Chantratita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanthiwa","family":"Wikraiphat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanaporn","family":"Wuthiekanun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zakiya","family":"Douglas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas P. J.","family":"Day","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Direk","family":"Limmathurotsakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Brett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary N.","family":"Burtnick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Malays Appl Biol","first-page":"1","article-title":"Burkholderia pseudomallei: an update on disease, virulence and host interaction","volume":"42","author":"Lee","year":"2013"},{"issue":"(Suppl 1)","key":"ref2","series-title":"Trans R Soc Trop Med Hyg","first-page":"S1","article-title":"The global distribution of Burkholderia pseudomallei and melioidosis: an update","volume":"102","author":"Currie","year":"2008"},{"key":"ref3","series-title":"Am J Trop Med Hyg","first-page":"1113","article-title":"Increasing incidence of human melioidosis in northeast Thailand","volume":"82","author":"Limmathurotsakul","year":"2010"},{"key":"ref4","series-title":"N Engl J Med","first-page":"1035","article-title":"Melioidosis","volume":"367","author":"Wiersinga","year":"2012"},{"key":"ref5","series-title":"Br Med Bull","first-page":"125","article-title":"Melioidosis: a clinical overview","volume":"99","author":"Limmathurotsakul","year":"2011"},{"key":"ref6","series-title":"Chest","first-page":"1420","article-title":"Pulmonary melioidosis","volume":"108","author":"Ip","year":"1995"},{"key":"ref7","series-title":"PLoS One","first-page":"e12485","article-title":"Defining the true sensitivity of culture for the diagnosis of melioidosis using Bayesian latent class models","volume":"5","author":"Limmathurotsakul","year":"2010"},{"key":"ref8","series-title":"Clin Vaccine Immunol","first-page":"110","article-title":"Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis","volume":"14","author":"Chantratita","year":"2007"},{"key":"ref9","series-title":"Melioidosis\u2014A Century of Observation and Research","first-page":"160","article-title":"The serological diagnosis of melioidosis","author":"Chantratita","year":"2012"},{"key":"ref10","series-title":"Emerg Infect Dis","article-title":"Melioidosis diagnostic workshop, 2013","volume":"21","author":"Hoffmaster","year":"2015"},{"key":"ref11","series-title":"Carbohydr Res","first-page":"90","article-title":"Structural analysis of capsular polysaccharides expressed by Burkholderia mallei and Burkholderia pseudomallei","volume":"349","author":"Heiss","year":"2012"},{"key":"ref12","series-title":"Carbohydr Res","first-page":"6","article-title":"Revised structures for the predominant O-polysaccharides expressed by Burkholderia pseudomallei and Burkholderia mallei","volume":"381","author":"Heiss","year":"2013"},{"key":"ref13","series-title":"Clin Infect Dis","first-page":"1024","article-title":"Enzyme-linked immunosorbent assay for the diagnosis of melioidosis: better than we thought","volume":"52","author":"Limmathurotsakul","year":"2011"},{"key":"ref14","series-title":"Carbohydr Res","first-page":"2783","article-title":"Application of carbohydrate microarray technology for the detection of Burkholderia pseudomallei, Bacillus anthracis and Francisella tularensis antibodies","volume":"343","author":"Parthasarathy","year":"2008"},{"key":"ref15","series-title":"Clin Infect Dis","first-page":"813","article-title":"Antilipopolysaccharide II: an antibody protective against fatal melioidosis","volume":"29","author":"Charuchaimontri","year":"1999"},{"key":"ref16","series-title":"Clin Infect Dis","first-page":"381","article-title":"Comparison of imipenem and ceftazidime as therapy for severe melioidosis","volume":"29","author":"Simpson","year":"1999"},{"key":"ref17","series-title":"Am J Trop Med Hyg","first-page":"348","article-title":"Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins","volume":"74","author":"Anuntagool","year":"2006"},{"key":"ref18","series-title":"Infect Immun","first-page":"3348","article-title":"Structural characterization of the lipopolysaccharide O antigens of Burkholderia pseudomallei","volume":"63","author":"Perry","year":"1995"},{"key":"ref19","series-title":"Front Cell Infect Microbiol","first-page":"108","article-title":"Development of capsular polysaccharide-based glycoconjugates for immunization against melioidosis and glanders","volume":"2","author":"Burtnick","year":"2012"},{"key":"ref20","series-title":"Front Cell Infect Microbiol","first-page":"148","article-title":"Development of novel O-polysaccharide based glycoconjugates for immunization against glanders","volume":"2","author":"Burtnick","year":"2012"},{"key":"ref21","series-title":"J Clin Microbiol","first-page":"239","article-title":"Serological evidence for increased human exposure to Burkholderia pseudomallei following the tsunami in southern Thailand","volume":"44","author":"Wuthiekanun","year":"2006"},{"key":"ref22","series-title":"Am J Trop Med Hyg","first-page":"1074","article-title":"Development of antibodies to Burkholderia pseudomallei during childhood in melioidosis-endemic northeast Thailand","volume":"74","author":"Wuthiekanun","year":"2006"},{"key":"ref23","series-title":"PLoS One","first-page":"e81617","article-title":"Survey of innate immune responses to Burkholderia pseudomallei in human blood identifies a central role for lipopolysaccharide","volume":"8","author":"Chantratita","year":"2013"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/93\/3\/article-p542.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/93\/3\/article-p542.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/93\/3\/article-p542.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T19:06:17Z","timestamp":1723057577000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/93\/3\/article-p542.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,2]]},"references-count":23,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.15-0114","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,2]]}}}