{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T22:40:05Z","timestamp":1723070405625},"reference-count":10,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,6]]},"abstract":"<jats:p>This report presents two cases of visceral leishmaniasis (VL) recurrence where the microscopy of the splenic smear failed in diagnosis. However, a strong clinical suspicion compelled further evaluation by polymerase chain reaction (PCR), which validated the etiology. This short report highlights the usefulness of PCR in diagnosing cases of suspected smear-negative VL recurrence.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0136","type":"journal-article","created":{"date-parts":[[2015,11,11]],"date-time":"2015-11-11T01:28:10Z","timestamp":1447205290000},"page":"99-101","source":"Crossref","is-referenced-by-count":2,"title":["Polymerase Chain Reaction in the Diagnosis of Visceral Leishmaniasis Recurrence in the Setting of Negative Splenic Smears"],"prefix":"10.4269","volume":"94","author":[{"given":"Golam","family":"Hasnain","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariful","family":"Basher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Proggananda","family":"Nath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prakash","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faria","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shakhawat","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinesh","family":"Mondal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"year":"2013","series-title":"Annual Report","author":"Bangladesh Disease Control Unit, Directorate General Health Service","key":"ref1"},{"key":"ref2","series-title":"Curr Trop Med Rep","first-page":"163","article-title":"Kala-azar (visceral leishmaniasis) elimination in Bangladesh: successes and challenges","volume":"1","author":"Ahmed","year":"2014"},{"key":"ref3","series-title":"HIV Med","first-page":"670","article-title":"\u201cActive chronic visceral leishmaniasis\u201d in HIV-1-infected patients demonstrated by biological and clinical long-term follow-up of 10 patients","volume":"11","author":"Bourgeois","year":"2010"},{"year":"2013","series-title":"National Guideline for Kala-azar Case Management","author":"Bangladesh Disease Control Unit, Directorate General Health Service","key":"ref4"},{"key":"ref5","series-title":"Trans R Soc Trop Med Hyg","first-page":"1","article-title":"Diagnosis of visceral leishmaniasis","volume":"105","author":"Srivastava","year":"2010"},{"key":"ref6","series-title":"J Clin Microbiol","first-page":"3038","article-title":"Evaluation of PCR for diagnosis of Indian kala-azar and assessment of cure","volume":"43","author":"Maurya","year":"2005"},{"key":"ref7","series-title":"Lancet Glob Health","first-page":"e51","article-title":"Efficacy and safety of single-dose liposomal amphotericin B for visceral leishmaniasis in a rural public hospital in Bangladesh: a feasibility study","volume":"2","author":"Mondal","year":"2014"},{"year":"1999","series-title":"Pediatric Reference Ranges","author":"Soldin","key":"ref8"},{"year":"2000","series-title":"Current Pediatric Diagnosis and Treatment","author":"Hay","key":"ref9"},{"key":"ref10","series-title":"Bangladesh J Med Sci","first-page":"216","article-title":"Detection of urinary leishmanial antigen by latex agglutination test (KAtex) in kala-azar patients","volume":"9","author":"Salam","year":"2010"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/94\/1\/article-p99.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/94\/1\/article-p99.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/94\/1\/article-p99.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T22:14:42Z","timestamp":1723068882000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/94\/1\/article-p99.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,6]]},"references-count":10,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.15-0136","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"type":"print","value":"0002-9637"},{"type":"electronic","value":"1476-1645"}],"subject":[],"published":{"date-parts":[[2016,1,6]]}}}