{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T01:03:01Z","timestamp":1775869381549,"version":"3.50.1"},"reference-count":12,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,10,7]]},"abstract":"<jats:p>Brucellosis remains a serious public health issue in developing countries, including China. On August 8, 2013, four cases of brucellosis from one extended family were reported at Shuyang County, Jiangsu Province, China. Active case finding was performed to identify the source and the risk factors of the infection and to prevent additional cases. Multiple-locus variable number tandem repeat analysis (MLVA) was used for molecular subtyping analysis. Six people from two extended families met the case definition for brucellosis infection; four were blood culture positive for <jats:italic>Brucella melitensis<\/jats:italic> biotype 3. Four additional family members were found seropositive by using a serological test. Isolates from the four patients were indistinguishable by MLVA profiling, displaying a unique type for Jiangsu Province. Field epidemiological data combined with MLVA genotyping supported a common source of the isolates from the different patients. We recommend stronger reinforcement measures for animal quarantine practices, enhanced cooperation with veterinary service organizations, and implementation of measures that strengthen public education on brucellosis to prevent further human outbreaks in Jiangsu Province.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0149","type":"journal-article","created":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T02:09:49Z","timestamp":1436234989000},"page":"757-760","source":"Crossref","is-referenced-by-count":18,"title":["A Familial Cluster of Human Brucellosis Attributable to Contact with Imported Infected Goats in Shuyang, Jiangsu Province, China, 2013"],"prefix":"10.4269","volume":"93","author":[{"given":"Zhongming","family":"Tan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weizhong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xilou","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zibing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fenyang","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yefei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Lancet Infect Dis","first-page":"91","article-title":"The new global map of human brucellosis","volume":"6","author":"Pappas","year":"2006"},{"key":"ref2","series-title":"N Engl J Med","first-page":"2325","article-title":"Brucellosis","volume":"352","author":"Pappas","year":"2013"},{"key":"ref3","series-title":"Emerg Infect Dis","first-page":"817","article-title":"Brucellosis in Guangdong Province, People's Republic of China, 2005\u20132010","volume":"19","author":"Chen","year":"2013"},{"key":"ref4","series-title":"Dis Surveill","first-page":"944","article-title":"Analysis on surveillance data of brucellosis in China, 2009 [in Chinese]","volume":"25","author":"Man","year":"2009"},{"key":"ref5","series-title":"J Med Microbiol","first-page":"1309","article-title":"Multiple genus-specific markers in PCR assays improve the specificity and sensitivity of diagnosis of brucellosis in field animals","volume":"56","author":"Mukherjee","year":"2007"},{"key":"ref6","series-title":"J Clin Microbiol","first-page":"2660","article-title":"Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR","volume":"32","author":"Bricker","year":"1994"},{"key":"ref7","series-title":"Int J Antimicrob","first-page":"S12","article-title":"Update on laboratory diagnosis of human brucellosis","volume":"36","author":"Araj","year":"2010"},{"key":"ref8","series-title":"BMC Microbiol","first-page":"9","article-title":"Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay","volume":"6","author":"Le Fl\u00e8che","year":"2006"},{"key":"ref9","series-title":"J Clin Microbiol","first-page":"1982","article-title":"Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp","volume":"44","author":"Whatmore","year":"2006"},{"key":"ref10","series-title":"BMC Microbiol","first-page":"256","article-title":"MLVA genotyping of Chinese human Brucella melitensis biovar 1, 2 and 3 isolates","volume":"11","author":"Jiang","year":"2011"},{"key":"ref11","series-title":"J Clin Microbiol","first-page":"3276","article-title":"Multiple-locus variable-number tandem-repeat analysis genotyping of human Brucella isolates from Turkey","volume":"49","author":"K\u0131l\u0131\u00e7","year":"2011"},{"key":"ref12","series-title":"Rev Sci Tech Oie","first-page":"271","article-title":"Brucellosis: \u2018One Health\u2019 challenges and opportunities","volume":"32","author":"Plumb","year":"2013"}],"container-title":["The American Society of Tropical Medicine and 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