{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:43:15Z","timestamp":1775058195966,"version":"3.50.1"},"reference-count":47,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,11,4]]},"abstract":"<jats:p>The resilience of <jats:italic>Burkholderia pseudomallei<\/jats:italic>, the causative agent of melioidosis, was evaluated in control soil microcosms and in soil microcosms containing NaCl or FeSO<jats:sub>4<\/jats:sub> at 30\u00b0C. Iron (Fe(II)) promoted the growth of <jats:italic>B. pseudomallei<\/jats:italic> during the 30-day observation, contrary to the presence of 1.5% and 3% NaCl. Scanning electron micrographs of <jats:italic>B. pseudomallei<\/jats:italic> in soil revealed their morphological alteration from rod to coccoid and the formation of microcolonies. The smallest <jats:italic>B. pseudomallei<\/jats:italic> cells were found in soil with 100 \u03bcM FeSO<jats:sub>4<\/jats:sub> compared with in the control soil or soil with 0.6% NaCl (<jats:italic>P<\/jats:italic> &lt; 0.05). The colony count on Ashdown's agar and bacterial viability assay using the LIVE\/DEAD<jats:sup>\u00ae<\/jats:sup> <jats:italic>Bac<\/jats:italic>Light<jats:sup>\u2122<\/jats:sup> stain combined with flow cytometry showed that <jats:italic>B. pseudomallei<\/jats:italic> remained culturable and viable in the control soil microcosms for at least 120 days. In contrast, soil with 1.5% NaCl affected their culturability at day 90 and their viability at day 120. Our results suggested that a low salinity and iron may influence the survival of <jats:italic>B. pseudomallei<\/jats:italic> and its ability to change from a rod-like to coccoid form. The morphological changes of <jats:italic>B. pseudomallei<\/jats:italic> cells may be advantageous for their persistence in the environment and may increase the risk of their transmission to humans.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0177","type":"journal-article","created":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T02:18:47Z","timestamp":1441073927000},"page":"1058-1065","source":"Crossref","is-referenced-by-count":21,"title":["Morphological Alteration and Survival of Burkholderia pseudomallei in Soil 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