{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T06:34:50Z","timestamp":1792132490872,"version":"4.4.0"},"reference-count":8,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,8,5]]},"abstract":"<jats:p><jats:italic>Leptospira<\/jats:italic> Vanaporn Wuthiekanun (LVW) agar was used to develop a disk diffusion assay for <jats:italic>Leptospira<\/jats:italic> spp. Ten pathogenic <jats:italic>Leptospira<\/jats:italic> isolates were tested, all of which were susceptible to 17 antimicrobial agents (amoxicillin\/clavulanic acid, amoxicillin, azithromycin, cefoxitin, ceftazidime, ceftriaxone, chloramphenicol, ciprofloxacin, clindamycin, doripenem, doxycycline, gentamicin, linezolid, nitrofurantoin, penicillin, piperacillin\/tazobactam, and tetracycline). All 10 isolates had no zone of growth inhibition for four antimicrobials (fosfomycin, nalidixic acid, rifampicin, and trimethoprim\/sulfamethoxazole). Of the ten <jats:italic>Leptospira<\/jats:italic>, seven had a growth inhibition zone of \u2264 21 mm for aztreonam, the zone diameter susceptibility break point for Enterobacteriaceae. This assay could find utility as a simple screening method during the epidemiological surveillance of antimicrobial resistance in <jats:italic>Leptospira<\/jats:italic> spp.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0180","type":"journal-article","created":{"date-parts":[[2015,6,10]],"date-time":"2015-06-10T13:03:20Z","timestamp":1433941400000},"page":"241-243","source":"Crossref","is-referenced-by-count":18,"title":["Antimicrobial Disk Susceptibility Testing of Leptospira spp. Using Leptospira Vanaporn Wuthiekanun (LVW) Agar"],"prefix":"10.4269","volume":"93","author":[{"given":"Vanaporn","family":"Wuthiekanun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Premjit","family":"Amornchai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayan","family":"Langla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J.","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas P. J.","family":"Day","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Direk","family":"Limmathurotsakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon J.","family":"Peacock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"R1","series-title":"Clin Microbiol Rev","first-page":"296","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001"},{"key":"R2","series-title":"Lancet Infect Dis","first-page":"757","article-title":"Leptospirosis: a zoonotic disease of global importance","volume":"3","author":"Bharti","year":"2003"},{"key":"R3","series-title":"Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control","author":"World Health Organization","year":"2003"},{"key":"R4","series-title":"Antimicrob Agents Chemother","first-page":"297","article-title":"Rapid isolation and susceptibility testing of Leptospira spp. using a new solid medium (LVW agar)","volume":"57","author":"Wuthiekanun","year":"2012"},{"key":"R5","series-title":"J Clin Microbiol","first-page":"4350","article-title":"Maintenance of Leptospira species in Leptospira Vanaporn Wuthiekanun agar","volume":"52","author":"Wuthiekanun","year":"2014"},{"key":"R6","series-title":"Am J Trop Med Hyg","first-page":"794","article-title":"Leptospira species in floodwater during the 2011 floods in the Bangkok Metropolitan Region, Thailand","volume":"89","author":"Thaipadungpanit","year":"2013"},{"key":"R7","series-title":"Antimicrob Agents Chemother","first-page":"4002","article-title":"Determination of susceptibilities of 26 Leptospira sp. serovars to 24 antimicrobial agents by a broth microdilution technique","volume":"48","author":"Murray","year":"2004"},{"key":"R8","series-title":"Antimicrob Agents Chemother","first-page":"5403","article-title":"In vitro sensitivity and resistance of 46 Leptospira strains isolated from rats in the Philippines to 14 antimicrobial agents","volume":"54","author":"Chakraborty","year":"2010"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/93\/2\/article-p241.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/93\/2\/article-p241.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/93\/2\/article-p241.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T19:13:45Z","timestamp":1723058025000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/93\/2\/article-p241.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,5]]},"references-count":8,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.15-0180","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,5]]}}}