{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:28:37Z","timestamp":1789187317123,"version":"build-2803163510"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,2,8]]},"abstract":"<jats:p>The loop-mediated isothermal amplification (LAMP) assay with its advantages of cost effectiveness, rapidity, and simplicity, has evolved as a sensitive and specific method for the detection of African trypanosomes. Highly sensitive LAMP reactions specific for <jats:italic>Trypanosoma brucei rhodesiense<\/jats:italic> or that recognize but do not discriminate between <jats:italic>Trypanosoma brucei brucei<\/jats:italic>, <jats:italic>T. b. rhodesiense<\/jats:italic>, <jats:italic>Trypanosoma brucei gambiense<\/jats:italic>, and <jats:italic>Trypanosoma evansi<\/jats:italic> have been developed. A sensitive LAMP assay targeting the <jats:italic>T. b. gambiense<\/jats:italic> 5.8S ribosomal RNA internal transcribed spacer 2 (5.8S-ITS2) gene is also available but this assay does not target binding sites that span the CCCA (C<jats:sub>3<\/jats:sub>A) (557\u2013560 bps) insertion site that further differentiates <jats:italic>T. b. gambiense<\/jats:italic> from <jats:italic>T. b. brucei<\/jats:italic>. Here we describe 5.8S-ITS2-targeted LAMP assay that fit these criteria. The LAMP primer sets containing the <jats:italic>T. b. gambiense<\/jats:italic>-specific C<jats:sub>3<\/jats:sub>A tetranucleotide at the start of the outer forward primer sequences showed high specificity and sensitivity down to at least 0.1 fg <jats:italic>T. b. gambiense<\/jats:italic> genomic DNA.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0288","type":"journal-article","created":{"date-parts":[[2016,6,7]],"date-time":"2016-06-07T03:04:20Z","timestamp":1465268660000},"page":"275-279","source":"Crossref","is-referenced-by-count":8,"title":["Loop-Mediated Isothermal Amplification for Detection of the 5.8S Ribosomal Ribonucleic Acid Internal Transcribed Spacer 2 Gene Found in Trypanosoma brucei gambiense"],"prefix":"10.4269","volume":"96","author":[{"given":"Olga V.","family":"Nikolskaia","sequence":"first","affiliation":[{"name":"Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriel M. M.","family":"Thekisoe","sequence":"additional","affiliation":[{"name":"Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Stephen","family":"Dumler","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis J.","family":"Grab","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Eur J Clin Microbiol Infect Dis","first-page":"905","article-title":"Human African trypanosomiasis in endemic populations and travellers","volume":"31","author":"Blum","year":"2012"},{"key":"ref2","series-title":"J Clin Invest","first-page":"496","article-title":"Human African trypanosomiasis of the CNS: current issues and challenges","volume":"113","author":"Kennedy","year":"2004"},{"key":"ref3","series-title":"African Trypanosomiasis in Man","author":"Molyneux","year":"1996"},{"key":"ref4","series-title":"Control and Surveillance of African Trypanosomiasis","first-page":"114","author":"World Health Organization","year":"1998"},{"key":"ref5","series-title":"J Neurol Sci","first-page":"127","article-title":"Blood-cerebrospinal fluid barrier and intrathecal immunoglobulins compared to field diagnosis of central nervous system involvement in sleeping sickness","volume":"193","author":"Bisser","year":"2002"},{"key":"ref6","series-title":"J Infect Chemother","first-page":"62","article-title":"Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases","volume":"15","author":"Mori","year":"2009"},{"key":"ref7","series-title":"Mol Cell Probes","first-page":"223","article-title":"Accelerated reaction by loop-mediated isothermal amplification using loop primers","volume":"16","author":"Nagamine","year":"2002"},{"key":"ref8","series-title":"Exp Parasitol","first-page":"333","article-title":"Simple, rapid, inexpensive platform for the diagnosis of malaria by loop mediated isothermal amplification (LAMP)","volume":"134","author":"Surabattula","year":"2013"},{"key":"ref9","series-title":"Biochem Biophys Res Commun","first-page":"150","article-title":"Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation","volume":"289","author":"Mori","year":"2001"},{"key":"ref10","series-title":"Nat Protoc","first-page":"877","article-title":"Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products","volume":"3","author":"Tomita","year":"2008"},{"key":"ref11","series-title":"Biotechnol Lett","first-page":"1939","article-title":"Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores","volume":"29","author":"Qiao","year":"2007"},{"key":"ref12","series-title":"PLoS Negl Trop Dis","first-page":"e865","article-title":"LAMP for human African trypanosomiasis: a comparative study of detection formats","volume":"4","author":"Wastling","year":"2010"},{"key":"ref13","series-title":"J Clin Microbiol","first-page":"5517","article-title":"Loop-mediated isothermal amplification for detection of African trypanosomes","volume":"41","author":"Kuboki","year":"2003"},{"key":"ref14","series-title":"Int J Parasitol","first-page":"589","article-title":"African trypanosomiasis: sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA","volume":"38","author":"Njiru","year":"2008"},{"key":"ref15","series-title":"PLoS Negl Trop Dis","first-page":"e147","article-title":"Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense","volume":"2","author":"Njiru","year":"2008"},{"key":"ref16","series-title":"Parasite Immunol","first-page":"361","article-title":"Regulation of innate and acquired immunity in African trypanosomiasis","volume":"27","author":"Mansfield","year":"2005"},{"key":"ref17","series-title":"Acta Trop","first-page":"182","article-title":"Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis","volume":"102","author":"Thekisoe","year":"2007"},{"key":"ref18","series-title":"Exp Parasitol","first-page":"123","article-title":"Measure of molecular diversity within the Trypanosoma brucei subspecies Trypanosoma brucei brucei and Trypanosoma brucei gambiense as revealed by genotypic characterization","volume":"99","author":"Agbo","year":"2001"},{"key":"ref19","series-title":"PLoS Negl Trop Dis","first-page":"e1249","article-title":"Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by loop-mediated isothermal amplification (LAMP)","volume":"5","author":"Grab","year":"2011"},{"key":"ref20","series-title":"Mol Diagn Ther","first-page":"339","article-title":"Role of circulating cell-free DNA in cancers","volume":"6","author":"Aarthy","year":"2015"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/96\/2\/article-p275.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/96\/2\/article-p275.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/96\/2\/article-p275.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T22:43:01Z","timestamp":1723070581000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/96\/2\/article-p275.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,8]]},"references-count":20,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.15-0288","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,8]]}}}