{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:32:46Z","timestamp":1787031166151,"version":"build-2736575974"},"reference-count":28,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,12,9]]},"abstract":"<jats:p>\n                    This study was performed to evaluate nested polymerase chain reaction (PCR) and real-time PCR methods for detection of\n                    <jats:italic>Strongyloides stercoralis<\/jats:italic>\n                    in fecal samples compared with parasitological methods. A total of 466 stool samples were examined by conventional parasitological methods (formalin ether concentration [FEC] and agar plate culture [APC]). DNA was extracted using an in-house method, and mitochondrial cytochrome c oxidase subunit 1 and 18S ribosomal genes were amplified by nested PCR and real-time PCR, respectively. Among 466 samples, 12.7% and 18.2% were found infected with\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    by FEC and APC, respectively. DNA of\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    was detected in 18.9% and 25.1% of samples by real-time PCR and nested PCR, respectively. Considering parasitological methods as the diagnostic gold standard, the sensitivity and specificity of nested PCR were 100% and 91.6%, respectively, and that of real-time PCR were 84.7% and 95.8%, respectively. However, considering sequence analyzes of the selected nested PCR products, the specificity of nested PCR is increased. In general, molecular methods were superior to parasitological methods. They were more sensitive and more reliable in detection of\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    in comparison with parasitological methods. Between the two molecular methods, the sensitivity of nested PCR was higher than real-time PCR.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.15-0309","type":"journal-article","created":{"date-parts":[[2015,9,8]],"date-time":"2015-09-08T22:29:07Z","timestamp":1441751347000},"page":"1285-1291","source":"Crossref","is-referenced-by-count":49,"title":["Comparison of Nested Polymerase Chain Reaction and Real-Time Polymerase Chain Reaction with Parasitological Methods for Detection of Strongyloides stercoralis in Human Fecal Samples"],"prefix":"10.4269","volume":"93","author":[{"given":"Meysam","family":"Sharifdini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Mirhendi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyhan","family":"Ashrafi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mostafa","family":"Hosseini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Mohebali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Khodadadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eshrat Beigom","family":"Kia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Clin Microbiol Rev","first-page":"208","article-title":"Strongyloides stercoralis in the immunocompromised population","volume":"17","author":"Keiser","year":"2004"},{"key":"ref2","series-title":"Clin Infect Dis","first-page":"1040","article-title":"Diagnosis of Strongyloides stercoralis infection","volume":"33","author":"Siddiqui","year":"2001"},{"key":"ref3","series-title":"Ann Pharmacother","first-page":"1992","article-title":"Manifestations, diagnosis, and treatment of Strongyloides stercoralis infection","volume":"41","author":"Segarra-Newnham","year":"2007"},{"key":"ref4","series-title":"Adv Parasitol","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0065-308X(08)60036-6","article-title":"Human strongyloidiasis","volume":"38","author":"Grove","year":"1996"},{"key":"ref5","series-title":"Curr 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mitochondrial mutants as an investigative tool to study human neurodegenerative diseases associated with mitochondrial dysfunction","volume":"2","author":"Ventura","year":"2007"},{"key":"ref27","series-title":"J Med Microbiol","first-page":"629","article-title":"Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients","volume":"53","author":"Hierl","year":"2004"},{"key":"ref28","series-title":"Avian Dis","first-page":"366","article-title":"Comparison of the specificity and sensitivity of PCR, nested PCR, and real-time PCR for the diagnosis of histomoniasis","volume":"49","author":"Hafez","year":"2005"}],"container-title":["The American Society of Tropical Medicine and 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