{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:08:08Z","timestamp":1791270488556,"version":"4.1.0"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,2,3]]},"abstract":"<jats:p>Chikungunya is caused by the mosquito-borne arthrogenic alphavirus, chikungunya virus (CHIKV). Chikungunya was introduced into the Americas in late 2013 and Nicaragua in mid-2014. Here, we sequenced five imported and 30 autochthonous Nicaraguan CHIKV from cases identified in the first epidemic in the country between August 2014 and April 2015. One full-length and two partial genomic sequences were obtained by deep sequencing; Sanger methodology yielded 33 <jats:italic>E1<\/jats:italic> sequences from five imported and 28 autochthonous cases. Phylogenetic analysis indicates that Nicaraguan CHIKV all belonged to the Asian genotype, Caribbean clade. Moreover, <jats:italic>E1<\/jats:italic> gene sequences revealed accumulation of mutations in later months of the epidemic, including four silent mutations in 11 autochthonous cases and three non-synonymous mutations in three autochthonous cases. No mutations contributing to increased transmissibility by <jats:italic>Aedes albopictus<\/jats:italic> were identified in the <jats:italic>E1<\/jats:italic> gene. This represents the most comprehensive set of CHIKV sequences available from the Americas to date.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0497","type":"journal-article","created":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T14:24:29Z","timestamp":1449584669000},"page":"400-403","source":"Crossref","is-referenced-by-count":18,"title":["Chikungunya Virus Sequences Across the First Epidemic in Nicaragua, 2014\u20132015"],"prefix":"10.4269","volume":"94","author":[{"given":"Chunling","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saira","family":"Saborio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Gresh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghana","family":"Eswarappa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Fire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poornima","family":"Parameswaran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Balmaseda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Emerg 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landscapes","volume":"108","author":"Tsetsarkin","year":"2011"},{"key":"ref20","series-title":"Nat Commun","first-page":"4084","article-title":"Multi-peaked adaptive landscape for chikungunya virus evolution predicts continued fitness optimization in Aedes albopictus mosquitoes","volume":"5","author":"Tsetsarkin","year":"2014"}],"container-title":["The American Society of Tropical Medicine and 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