{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T05:59:47Z","timestamp":1793339987011,"version":"4.5.0"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,2,3]]},"abstract":"<jats:p>The lack of rapid, affordable, and accurate diagnostic tests represents the primary hurdle affecting malaria surveillance in resource- and expertise-limited areas. Loop-mediated isothermal amplification (LAMP) is a sensitive, rapid, and cheap diagnostic method. Five species-specific LAMP assays were developed based on <jats:italic>18S rRNA<\/jats:italic> gene. Sensitivity and specificity of LAMP results were calculated as compared with microscopic examination and nested polymerase chain reaction. LAMP reactions were highly sensitive with the detection limit of one copy for <jats:italic>Plasmodium vivax<\/jats:italic>, <jats:italic>Plasmodium falciparum<\/jats:italic>, and <jats:italic>Plasmodium malariae<\/jats:italic> and 10 copies for <jats:italic>Plasmodium knowlesi<\/jats:italic> and <jats:italic>Plasmodium ovale<\/jats:italic>. LAMP positively detected all human malaria species in all positive samples (<jats:italic>N<\/jats:italic> = 134; sensitivity = 100%) within 35 minutes. All negative samples were not amplified by LAMP (<jats:italic>N<\/jats:italic> = 67; specificity = 100%). LAMP successfully detected two samples with very low parasitemia. LAMP may offer a rapid, simple, and reliable test for the diagnosis of malaria in areas where malaria is prevalent.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0569","type":"journal-article","created":{"date-parts":[[2015,11,24]],"date-time":"2015-11-24T03:15:54Z","timestamp":1448334954000},"page":"336-339","source":"Crossref","is-referenced-by-count":69,"title":["Loop-Mediated Isothermal Amplification Assay for Identification of Five Human Plasmodium Species in Malaysia"],"prefix":"10.4269","volume":"94","author":[{"given":"Yee-Ling","family":"Lau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Yee","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mun-Yik","family":"Fong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenarun","family":"Jelip","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohela","family":"Mahmud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"World Malaria Report: 2014","author":"World Health Organization","year":"2014"},{"key":"ref2","series-title":"J Clin Microbiol","first-page":"1491","article-title":"Malaria rapid diagnostic tests: challenges and 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malaria infections by microscopy, nested PCR, and LAMP in northern Thailand","volume":"83","author":"P\u00f6schl","year":"2010"},{"key":"ref19","series-title":"Clin Microbiol Rev","first-page":"165","article-title":"Human infections and detection of Plasmodium knowlesi","volume":"26","author":"Singh","year":"2013"},{"key":"ref20","series-title":"Nucleic Acids Res","first-page":"E63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000"}],"container-title":["The American Society of Tropical Medicine and 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