{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:36:55Z","timestamp":1790033815350,"version":"4.0.1"},"reference-count":16,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,9,7]]},"abstract":"<jats:p>Mansonellosis is endemic in several regions of Africa, the Caribbean, and Latin America. <jats:italic>Mansonella ozzardi<\/jats:italic> and <jats:italic>Mansonella perstans<\/jats:italic> have been reported in Latin America, including the Amazon region. A morphological and molecular microfilariae study was performed in Pauini (Brazil). Blood samples were collected from 40 individuals, and were analyzed by Giemsa-stained blood film and by two different nested polymerase chain reactions which detect internal transcribed spacer-1 and the major sperm protein gene. By microscopy, 14 of 40 were positive: 11 as <jats:italic>M. ozzardi<\/jats:italic> and three as <jats:italic>M. perstans<\/jats:italic>\u2013like infections. Both molecular methods detected 19 positive cases as <jats:italic>M. ozzardi<\/jats:italic>, including those 14 individuals detected by microscopy, without detectable genetic differences among any of the 19 positive samples. Molecular techniques showed an improvement of mansonellosis diagnosis and may become an effective tool to evaluate the present status of <jats:italic>M. ozzardi<\/jats:italic> and <jats:italic>M. perstans<\/jats:italic> in Latin America.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0654","type":"journal-article","created":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T06:25:38Z","timestamp":1468304738000},"page":"629-632","source":"Crossref","is-referenced-by-count":19,"title":["Atypical Mansonella ozzardi Microfilariae from an Endemic Area of Brazilian Amazonia"],"prefix":"10.4269","volume":"95","author":[{"given":"Thuy-Huong","family":"Ta-Tang","sequence":"first","affiliation":[{"name":"1Malaria and Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Instituto de Salud Carlos III, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio L. B.","family":"Luz","sequence":"additional","affiliation":[{"name":"2Instituto Le\u00f4nidas e Maria Deane, Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ) Amaz\u00f4nia, Manaus, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco J.","family":"Merino","sequence":"additional","affiliation":[{"name":"3Severo Ochoa University Hospital, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"de Fuentes","sequence":"additional","affiliation":[{"name":"1Malaria and Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Instituto de Salud Carlos III, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogelio","family":"L\u00f3pez-V\u00e9lez","sequence":"additional","affiliation":[{"name":"4Tropical Medicine and Clinical Parasitology Unit, Infectious Diseases Department, Hospital Ram\u00f3n y Cajal, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana A. P.","family":"Almeida","sequence":"additional","affiliation":[{"name":"2Instituto Le\u00f4nidas e Maria Deane, Funda\u00e7\u00e3o Oswaldo Cruz (FIOCRUZ) Amaz\u00f4nia, Manaus, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Lanza","sequence":"additional","affiliation":[{"name":"1Malaria and Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Instituto de Salud Carlos III, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udia M. M.","family":"Abrahim","sequence":"additional","affiliation":[{"name":"5Funda\u00e7\u00e3o de Hematologia e Hemoterapia do Amazonas (HEMOAM), Manaus, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"Rubio","sequence":"additional","affiliation":[{"name":"1Malaria and Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Instituto de Salud Carlos III, Madrid, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Adv Parasitol","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0065-308X(03)53004-4","article-title":"Diagnosis of human filariases (except onchocerciasis)","volume":"53","author":"Walther","year":"2003"},{"key":"ref2","series-title":"Clin Infect Dis","first-page":"1103","article-title":"Laboratory diagnosis of infections due to blood and tissue 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Biosci","first-page":"569","article-title":"TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment","volume":"10","author":"Van de Peer","year":"1994"},{"key":"ref16","series-title":"Mem Inst Oswaldo Cruz","first-page":"506","article-title":"Outstanding insecurities concerning the use of an Ov16-based ELISA in the Amazonia onchocerciasis focus","volume":"109","author":"Luz","year":"2014"}],"container-title":["The American Society of Tropical Medicine and 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