{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:32:44Z","timestamp":1762252364721},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,7,6]]},"abstract":"<jats:p>The simian parasite <jats:italic>Plasmodium knowlesi<\/jats:italic> is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of <jats:italic>Plasmodium<\/jats:italic> species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of <jats:italic>P. knowlesi<\/jats:italic> requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of <jats:italic>P. knowlesi.<\/jats:italic> This assay is able to detect 0.2 parasites\/\u03bcL, and compared with PCR has a sensitivity of 96% for the detection of <jats:italic>P. knowlesi<\/jats:italic>, making it a potentially field-applicable point-of-care diagnostic tool.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0670","type":"journal-article","created":{"date-parts":[[2016,5,10]],"date-time":"2016-05-10T03:05:26Z","timestamp":1462849526000},"page":"120-122","source":"Crossref","is-referenced-by-count":17,"title":["A Sensitive, Colorimetric, High-Throughput Loop-Mediated Isothermal Amplification Assay for the Detection of Plasmodium knowlesi"],"prefix":"10.4269","volume":"95","author":[{"given":"Sumudu","family":"Britton","sequence":"first","affiliation":[{"name":"University of Queensland, Brisbane, Australia."},{"name":"QIMR Berghofer Medical Research Institute, Brisbane, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Australian Army Malaria Institute, Brisbane, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew J.","family":"Grigg","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"William","sequence":"additional","affiliation":[{"name":"Clinical Research Centre, Queen Elizabeth Hospital, Kota Kinabalu, Sabah, Malaysia."},{"name":"Jesselton Medical Center, Kota Kinabalu, Sabah, Malaysia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas M.","family":"Anstey","sequence":"additional","affiliation":[{"name":"Menzies School of Health Research, Charles Darwin University, Darwin, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James S.","family":"McCarthy","sequence":"additional","affiliation":[{"name":"University of Queensland, Brisbane, Australia."},{"name":"QIMR Berghofer Medical Research Institute, Brisbane, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Clin Microbiol Rev","first-page":"165","article-title":"Human infections and detection of Plasmodium knowlesi","volume":"26","author":"Singh","year":"2013"},{"key":"ref2","series-title":"Malar J","first-page":"390","article-title":"Changing epidemiology of malaria in Sabah, Malaysia: increasing incidence of Plasmodium knowlesi","volume":"13","author":"William","year":"2014"},{"key":"ref3","series-title":"Malar J","first-page":"8","article-title":"Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum, Plasmodium vivax, and Plasmodium knowlesi","volume":"12","author":"Barber","year":"2013"},{"key":"ref4","series-title":"J Clin Microbiol","first-page":"1118","article-title":"Evaluation of the sensitivity of a pLDH-based and an aldolase-based rapid diagnostic test for diagnosis of uncomplicated and severe malaria caused by PCR-confirmed Plasmodium knowlesi, Plasmodium falciparum, and Plasmodium vivax","volume":"51","author":"Barber","year":"2013"},{"key":"ref5","series-title":"Emerg Infect Dis","first-page":"1248","article-title":"Severe Plasmodium knowlesi malaria in a tertiary care hospital, Sabah, Malaysia","volume":"17","author":"William","year":"2011"},{"key":"ref6","series-title":"Clin Infect Dis","first-page":"383","article-title":"A prospective comparative study of knowlesi, falciparum, and vivax malaria in Sabah, Malaysia: high proportion with severe disease from Plasmodium knowlesi and Plasmodium vivax but no mortality with early referral and artesunate therapy","volume":"56","author":"Barber","year":"2013"},{"key":"ref7","series-title":"J Clin Microbiol","first-page":"2053","article-title":"Combining parasite lactate dehydrogenase-based and histidine-rich protein 2-based rapid tests to improve specificity for diagnosis of malaria due to Plasmodium knowlesi and other Plasmodium species in Sabah, Malaysia","volume":"52","author":"Grigg","year":"2014"},{"key":"ref8","series-title":"J Clin Microbiol","first-page":"4173","article-title":"Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect Plasmodium knowlesi","volume":"47","author":"Imwong","year":"2009"},{"key":"ref9","series-title":"Clin Microbiol Infect","first-page":"1484","article-title":"Improved performance with saliva and urine as alternative DNA sources for malaria diagnosis by mitochondrial DNA-based PCR assays","volume":"17","author":"Putaporntip","year":"2011"},{"key":"ref10","series-title":"PLoS One","first-page":"e31848","article-title":"A new single-step PCR assay for the detection of the zoonotic malaria parasite Plasmodium knowlesi","volume":"7","author":"Lucchi","year":"2012"},{"key":"ref11","series-title":"Malar J","first-page":"344","article-title":"A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi","volume":"9","author":"Divis","year":"2010"},{"key":"ref12","series-title":"J Clin Microbiol","first-page":"2931","article-title":"Multiplex 5\u2032 nuclease quantitative real-time PCR for clinical diagnosis of malaria and species-level identification and epidemiologic evaluation of malaria-causing parasites, including Plasmodium knowlesi","volume":"51","author":"Reller","year":"2013"},{"key":"ref13","series-title":"Am J Trop Med Hyg","first-page":"721","article-title":"A modified semi-nested multiplex malaria PCR (SnM-PCR) for the identification of the five human Plasmodium species occurring in southeast Asia","volume":"89","author":"Van Hong","year":"2013"},{"key":"ref14","series-title":"J Clin Microbiol","first-page":"2509","article-title":"Evaluation of a loop-mediated isothermal amplification method as a tool for diagnosis of infection by the zoonotic simian malaria parasite Plasmodium knowlesi","volume":"48","author":"Iseki","year":"2010"},{"key":"ref15","series-title":"Malar J","first-page":"197","article-title":"Specific, sensitive, and rapid detection of human Plasmodium knowlesi infection by loop-mediated isothermal amplification (LAMP) in blood samples","volume":"10","author":"Lau","year":"2011"},{"key":"ref16","series-title":"PLoS Negl Trop Dis","first-page":"e0004443","article-title":"Sensitive detection of Plasmodium vivax using a high-throughput, colourimetric loop mediated isothermal amplification (HtLAMP) platform: a potential novel tool for malaria elimination","volume":"10","author":"Britton","year":"2016"},{"key":"ref17","series-title":"Lancet Infect Dis","first-page":"180","article-title":"Artesunate-mefloquine versus chloroquine for treatment of uncomplicated Plasmodium knowlesi malaria in Malaysia (ACT KNOW): an open-label, randomised controlled trial","volume":"16","author":"Grigg","year":"2016"},{"key":"ref18","series-title":"Ann Trop Med Parasitol","first-page":"131","article-title":"Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present","volume":"97","author":"Padley","year":"2003"},{"key":"ref19","series-title":"Malar J","first-page":"335","article-title":"A simple, high-throughput, colourimetric, field applicable loop-mediated isothermal amplification (HtLAMP) assay for malaria elimination","volume":"14","author":"Britton","year":"2015"},{"key":"ref20","series-title":"J Infect Dis","first-page":"784","article-title":"Asymptomatic and submicroscopic carriage of Plasmodium knowlesi malaria in household and community members of clinical cases in Sabah, Malaysia","volume":"213","author":"Fornace","year":"2016"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/95\/1\/article-p120.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/95\/1\/article-p120.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/95\/1\/article-p120.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T22:03:29Z","timestamp":1723068209000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/95\/1\/article-p120.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,6]]},"references-count":20,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.15-0670","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"type":"print","value":"0002-9637"},{"type":"electronic","value":"1476-1645"}],"subject":[],"published":{"date-parts":[[2016,7,6]]}}}