{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:43:33Z","timestamp":1785365013829,"version":"3.55.0"},"reference-count":31,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,6,1]]},"abstract":"<jats:p>For differential detection of <jats:italic>Taenia solium<\/jats:italic>, <jats:italic>Taenia saginata<\/jats:italic>, and <jats:italic>Taenia asiatica<\/jats:italic>, loop-mediated isothermal amplification (LAMP) assay targeting the cytochrome <jats:italic>c<\/jats:italic> oxidase subunit 1 gene has been recently developed and shown to be sensitive, specific, and effective. However, to achieve differential identification, one specimen requires three reaction mixtures containing a primer set of each <jats:italic>Taenia<\/jats:italic> species separately, which is complex and time consuming and increases the risk of cross-contamination. In this study, we developed a simple differential identification of human <jats:italic>Taenia<\/jats:italic> species using multiplex LAMP (mLAMP) in combination with dot enzyme-linked immunosorbent assay (dot-ELISA). Forward inner primers of <jats:italic>T. solium<\/jats:italic>, <jats:italic>T. saginata<\/jats:italic>, and <jats:italic>T. asiatica<\/jats:italic> labeled with fluorescein isothiocyanate (FITC), digoxigenin (DIG), and tetramethylrhodamine (TAMRA), respectively, and biotin-labeled backward inner primers were used in mLAMP. The mLAMP assay succeeded in specific amplification of each respective target gene in a single tube. Furthermore, the mLAMP product from each species was easily distinguished by dot-ELISA with an antibody specific for FITC, DIG, or TAMRA. The mLAMP assay in combination with dot-ELISA will make identification of human <jats:italic>Taenia<\/jats:italic> species simpler, easier, and more practical.<\/jats:p>","DOI":"10.4269\/ajtmh.15-0829","type":"journal-article","created":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T02:33:05Z","timestamp":1459823585000},"page":"1318-1323","source":"Crossref","is-referenced-by-count":29,"title":["Simple Identification of Human Taenia Species by Multiplex Loop-Mediated Isothermal Amplification in Combination with Dot Enzyme-Linked Immunosorbent Assay"],"prefix":"10.4269","volume":"94","author":[{"given":"Agathe","family":"Nkouawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhito","family":"Sako","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munehiro","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Lancet","first-page":"547","article-title":"Taenia solium cysticercosis","volume":"362","author":"Garcia","year":"2003"},{"issue":"(Suppl 4)","key":"ref2","series-title":"Southeast Asian J Trop Med Public Health","first-page":"S123","article-title":"Taeniasis and cysticercosis in Asia and the Pacific: present state of knowledge and perspectives","volume":"36","author":"Ito","year":"2005"},{"key":"ref3","series-title":"First WHO Report on Neglected Tropical Diseases: Working to Overcome the Global Impact of Neglected Tropical Diseases","first-page":"97","author":"World Health Organization","year":"2010"},{"key":"ref4","series-title":"Pathog Glob Health","first-page":"261","article-title":"Neurocysticercosis in sub-Saharan Africa: a review of prevalence, clinical characteristics, diagnosis, and management","volume":"106","author":"Winkler","year":"2012"},{"key":"ref5","series-title":"Acta Trop","first-page":"223","article-title":"Taeniasis\/cysticercosis in a Tibetan population in Sichuan Province, China","volume":"100","author":"Li","year":"2006"},{"key":"ref6","series-title":"Recent Pat Antiinfect Drug Discov","first-page":"173","article-title":"The present situation of human taeniases and cysticercosis in Asia","volume":"9","author":"Ito","year":"2014"},{"key":"ref7","series-title":"N Engl J Med","first-page":"692","article-title":"Neurocysticercosis in an Orthodox Jewish community in New York City","volume":"327","author":"Schantz","year":"1992"},{"key":"ref8","series-title":"Emerg Infect Dis","first-page":"1","article-title":"Public health implications of cysticercosis acquired in the United States","volume":"17","author":"Sorvillo","year":"2011"},{"key":"ref9","series-title":"Parasit Vectors","first-page":"18","article-title":"Taeniasis and cysticercosis due to Taenia solium in Japan","volume":"5","author":"Yanagida","year":"2012"},{"key":"ref10","series-title":"WHO\/FAO\/OIE Guidelines for the Surveillance, Prevention and Control of Taeniosis\/Cysticercosis","first-page":"27","article-title":"Epidemiology of taeniosis and cysticercosis","author":"Murrell","year":"2005"},{"key":"ref11","series-title":"Oxford Textbook of Zoonoses, Biology, Clinical Practice and Public Health Control","first-page":"627","article-title":"Cysticercosis and taeniosis: Taenia solium, Taenia saginata, and Taenia asiatica","author":"Flisser","year":"2011"},{"key":"ref12","series-title":"Parasitology","first-page":"1602","article-title":"Detection of human taeniases in Tibetan endemic areas, China","volume":"140","author":"Li","year":"2013"},{"key":"ref13","series-title":"Parasitology","first-page":"1608","article-title":"Taeniases and cysticercosis in Indonesia: past and present situations","volume":"140","author":"Wandra","year":"2013"},{"key":"ref14","series-title":"J Infect Chemother","first-page":"62","article-title":"Loop-mediated isothermal amplification (LAMP), a rapid, accurate, and cost-effective diagnostic method for infectious diseases","volume":"15","author":"Mori","year":"2009"},{"key":"ref15","series-title":"J Infect Chemother","first-page":"404","article-title":"Loop-mediated isothermal amplification (LAMP): recent progress in research and development","volume":"19","author":"Mori","year":"2013"},{"key":"ref16","series-title":"J Clin Microbiol","first-page":"168","article-title":"Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species","volume":"47","author":"Nkouawa","year":"2009"},{"key":"ref17","series-title":"Nuclear Acid Detection, Methods and Protocols","first-page":"109","article-title":"Chapter 9. Loop-mediated isothermal amplification method for a differential identification of human Taenia tapeworms","volume":"Vol. 1039","author":"Sako","year":"2013"},{"key":"ref18","series-title":"J Clin Microbiol","first-page":"3350","article-title":"Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of Taenia species from humans","volume":"48","author":"Nkouawa","year":"2010"},{"key":"ref19","series-title":"Parasitol Int","first-page":"723","article-title":"Loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey","volume":"61","author":"Nkouawa","year":"2012"},{"key":"ref20","series-title":"J Clin Microbiol","first-page":"548","article-title":"DNA differential diagnosis of taeniasis and cysticercosis by multiplex PCR","volume":"42","author":"Yamasaki","year":"2004"},{"issue":"(Suppl)","key":"ref21","series-title":"Parasitol Int","first-page":"S91","article-title":"Usefulness of severe combined immunodeficiency (scid) and inbred mice for studies of cysticercosis and echinococcosis","volume":"55","author":"Nakaya","year":"2006"},{"key":"ref22","series-title":"J Microbiol Methods","first-page":"281","article-title":"Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites","volume":"71","author":"Iseki","year":"2007"},{"key":"ref23","series-title":"J Microbiol Methods","first-page":"174","article-title":"Evaluation of reverse transcription loop-mediated isothermal amplification in conjunction with ELISA-hybridization assay for molecular detection of Mycobacterium tuberculosis","volume":"76","author":"Lee","year":"2009"},{"key":"ref24","series-title":"J Microbiol Methods","first-page":"53","article-title":"One-tube loop-mediated isothermal amplification combined with restriction endonuclease digestion and ELISA for colorimetric detection of resistance to isoniazid, ethambutol and streptomycin in Mycobacterium tuberculosis isolates","volume":"83","author":"Lee","year":"2010"},{"key":"ref25","series-title":"J Clin Virol","first-page":"127","article-title":"Multiplex loop-mediated isothermal amplification (M-LAMP) assay for the detection of influenza A\/H1, A\/H3 and influenza B can provide a specimen-to-result diagnosis in 40 min with single genome copy sensitivity","volume":"58","author":"Mahony","year":"2013"},{"key":"ref26","series-title":"J Virol Methods","first-page":"18","article-title":"Development and evaluation of multiplex RT-LAMP assays for rapid and sensitive detection of foot-and-mouth disease virus","volume":"192","author":"Yamazaki","year":"2013"},{"key":"ref27","series-title":"Diagn Microbiol Infect Dis","first-page":"205","article-title":"Rapid and sensitive detection of human African trypanosomiasis by loop-mediated isothermal amplification combined with a lateral-flow dipstick","volume":"69","author":"Njiru","year":"2011"},{"key":"ref28","series-title":"BioMed Res Int","first-page":"926230","article-title":"Detection of Mycobacterium tuberculosis by using loop-mediated isothermal amplification combined with a lateral flow dipstick in clinical samples","volume":"2013","author":"Kaewphinit","year":"2013"},{"key":"ref29","series-title":"Parasitol Int","first-page":"777","article-title":"Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax","volume":"63","author":"Yongkiettrakul","year":"2014"},{"key":"ref30","series-title":"Anal Chim Acta","first-page":"541","article-title":"Combination of multiplex reverse-transcription loop-mediated isothermal amplification with an immunochromatographic strip for subtyping influenza A virus","volume":"853","author":"Jung","year":"2015"},{"key":"ref31","series-title":"Anal Chim Acta","first-page":"142","article-title":"Development of multiplex loop mediated isothermal amplification (m-LAMP) label-based gold nanoparticles lateral flow dipstick biosensor for detection of pathogenic Leptospira","volume":"903","author":"Nurul Najian","year":"2016"}],"container-title":["The American Society of Tropical Medicine and 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