{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:02:17Z","timestamp":1761541337146},"reference-count":53,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,11,2]]},"abstract":"<jats:p>The purpose of this study was to determine the in vitro and ex vivo susceptibility of human corneal cells to West Nile virus (WNV) infection and evaluate the ability of the virus to disseminate to the corneas of infected mice. Human corneal epithelial cells were challenged with WNV, incubated for 1\u20136 days, and tested for evidence of WNV infection. Viral RNA and antigen were detected at every time point, and the virus reached a peak titer of 2.5 \u00d7 10<jats:sup>7<\/jats:sup> plaque-forming units (pfu)\/mL at 3 days postinoculation (PI). Corneas procured from donors were incubated in culture dishes containing WNV for 1\u20135 days and tested for evidence of WNV. Viral RNA and antigen were detected, and the virus reached a mean peak titer of 4.9 \u00d7 10<jats:sup>4<\/jats:sup> pfu\/mL at 5 days PI. Mice were inoculated intraperitoneally with WNV, and their eyes were harvested at 2, 5, and 8 days PI and tested for evidence of WNV. Viral RNA was detected in corneas of four of nine systemically infected mice as early as 2 days PI. We conclude that human corneal cells support WNV replication in vitro and ex vivo, and WNV may disseminate into the corneas of experimentally infected mice. These findings indicate that corneal transmission cannot be ruled out as a novel mode of human-to-human WNV transmission and additional experiments should be conducted to assess this risk further.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0256","type":"journal-article","created":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T03:48:47Z","timestamp":1474948127000},"page":"1185-1191","source":"Crossref","is-referenced-by-count":4,"title":["West Nile Virus Infection in Human and Mouse Cornea Tissue"],"prefix":"10.4269","volume":"95","author":[{"given":"Bradley J.","family":"Blitvich","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas."},{"name":"Department of Pathology, University of Texas Medical Branch, Galveston, Texas."},{"name":"Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vandana","family":"Saxena","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shemin","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, Iowa."},{"name":"Stephen A. Wynn Institute for Vision Research, University of Iowa, Iowa City, Iowa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen M.","family":"Harmon","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew D.","family":"Raymond","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth M.","family":"Goins","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, Iowa."},{"name":"Iowa Lions Eye Bank, Coralville, Iowa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia R.","family":"Reed","sequence":"additional","affiliation":[{"name":"Iowa Lions Eye Bank, Coralville, Iowa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert F.","family":"Mullins","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, Iowa."},{"name":"Stephen A. Wynn Institute for Vision Research, University of Iowa, Iowa City, Iowa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Greiner","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, Iowa."},{"name":"Stephen A. Wynn Institute for Vision Research, University of Iowa, Iowa City, Iowa."},{"name":"Iowa Lions Eye Bank, Coralville, Iowa."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"JAMA","first-page":"308","article-title":"West Nile virus: review of the literature","volume":"310","author":"Petersen","year":"2013"},{"key":"ref2","series-title":"Viruses","first-page":"3021","article-title":"Vector-virus interactions and transmission dynamics of West Nile virus","volume":"5","author":"Ciota","year":"2013"},{"key":"ref3","series-title":"Clin Microbiol Rev","first-page":"635","article-title":"West Nile virus: biology, transmission, and human infection","volume":"25","author":"Colpitts","year":"2012"},{"key":"ref4","series-title":"J Infect Dis","first-page":"1354","article-title":"West Nile fever characteristics among viremic persons identified through blood donor 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