{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:33:35Z","timestamp":1761863615645},"reference-count":23,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,10,5]]},"abstract":"<jats:p><jats:italic>Burkholderia pseudomallei<\/jats:italic> is a gram-negative bacterium that causes melioidosis. In this study, we examined the sequence types (STs) of 60 clinical isolates from patients with melioidosis in Hainan, a tropical island in southern China. The 60 clinical isolates were resolved into 30 STs. Among the STs, ST562 was also reported in Australia, and ST90 was also reported in the United States, probably from a traveler who had previously visited Asia. In addition, six novel STs were found in this study, including ST1395, ST1396, ST1397, ST1398, ST1399, and ST1443, whereas the remaining STs were mostly shared with other southeast Asian regions. Phylogenetic analysis of 60 isolates conducted using the eBURST v3 software segregated the 30 STs into three groups and 18 singletons. Our study shows genetic diversity of 60 clinical isolates of <jats:italic>B. pseudomallei<\/jats:italic> in Hainan Island.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0280","type":"journal-article","created":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T02:38:47Z","timestamp":1468895927000},"page":"760-764","source":"Crossref","is-referenced-by-count":12,"title":["Multilocus Sequence Typing of Clinical Isolates of Burkholderia pseudomallei Collected in Hainan, a Tropical Island of Southern China"],"prefix":"10.4269","volume":"95","author":[{"given":"Xu-Ming","family":"Wang","sequence":"first","affiliation":[{"name":"1Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Zheng","sequence":"additional","affiliation":[{"name":"3State Key Laboratory for Infectious Disease Prevention and Control, Beijing, People's Republic of China."},{"name":"4National Institute for Communicable Disease Control and Prevention, Beijing, People's Republic of China."},{"name":"5Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Wu","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Jun","family":"Zhou","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui-Hui","family":"Kuang","sequence":"additional","affiliation":[{"name":"2Hainan Department, The General Hospital of the Chinese People's Liberation Army, Sanya, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Li","family":"Guo","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Xu","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian-Jiao","family":"Li","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling-Li","family":"Liu","sequence":"additional","affiliation":[{"name":"1Department of Clinical Laboratory, Hainan General Hospital, Haikou, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"3State Key Laboratory for Infectious Disease Prevention and Control, Beijing, People's Republic of China."},{"name":"4National Institute for Communicable Disease Control and Prevention, Beijing, People's Republic of China."},{"name":"5Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, People's Republic of China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"PLoS Negl Trop Dis","first-page":"e364","article-title":"Landscape changes influence the occurrence of the melioidosis bacterium Burkholderia pseudomallei in soil in northern Australia","volume":"3","author":"Kaestli","year":"2009"},{"key":"ref2","series-title":"Appl Environ Microbiol","first-page":"7243","article-title":"Groundwater seeps facilitate exposure to Burkholderia pseudomallei","volume":"77","author":"Baker","year":"2011"},{"key":"ref3","series-title":"Appl Environ Microbiol","first-page":"532","article-title":"Randomized soil survey of the distribution of Burkholderia pseudomallei in rice fields in Laos","volume":"77","author":"Rattanavong","year":"2011"},{"key":"ref4","series-title":"Appl Environ Microbiol","first-page":"876","article-title":"Effectiveness of a simplified method for isolation of Burkholderia pseudomallei from soil","volume":"78","author":"Limmathurotsakul","year":"2012"},{"key":"ref5","series-title":"Am J Trop Med Hyg","first-page":"872","article-title":"Burkholderia pseudomallei detection in surface water in southern Laos using Moore's swabs","volume":"86","author":"Vongphayloth","year":"2012"},{"key":"ref6","series-title":"Emerg Infect Dis","first-page":"1538","article-title":"Intensity of rainfall and severity of melioidosis, Australia","volume":"9","author":"Currie","year":"2003"},{"key":"ref7","series-title":"Emerg Infect Dis","first-page":"1322","article-title":"Typhoon-related leptospirosis and melioidosis, Taiwan, 2009","volume":"17","author":"Su","year":"2011"},{"key":"ref8","series-title":"Med J Aust","first-page":"345","article-title":"Melioidosis at Royal Darwin Hospital in the big 2009\u20132010 wet season: comparison with the preceding 20 years","volume":"196","author":"Parameswaran","year":"2012"},{"key":"ref9","series-title":"Emerg Infect Dis","first-page":"159","article-title":"Association of melioidosis incidence with rainfall and humidity, Singapore, 2003\u20132012","volume":"21","author":"Liu","year":"2015"},{"key":"ref10","series-title":"PLoS Negl Trop Dis","first-page":"e0003834","article-title":"Airborne transmission of melioidosis to humans from environmental aerosols contaminated with B. pseudomallei","volume":"9","author":"Chen","year":"2015"},{"key":"ref11","series-title":"Int J Epidemiol","first-page":"1082","article-title":"The epidemiology of melioidosis in Ubon Ratchatani, northeast Thailand","volume":"23","author":"Suputtamongkol","year":"1994"},{"key":"ref12","series-title":"Am J Trop Med Hyg","first-page":"365","article-title":"Melioidosis as a consequence of sporting activity","volume":"89","author":"Hill","year":"2013"},{"key":"ref13","series-title":"Clin Infect Dis","first-page":"243","article-title":"Contact investigation of melioidosis cases reveals regional endemicity in Puerto Rico","volume":"60","author":"Doker","year":"2015"},{"key":"ref14","series-title":"Lancet","first-page":"1715","article-title":"Melioidosis","volume":"361","author":"White","year":"2003"},{"key":"ref15","series-title":"Clin Microbiol Rev","first-page":"383","article-title":"Melioidosis: epidemiology, pathophysiology, and management","volume":"18","author":"Cheng","year":"2005"},{"key":"ref16","series-title":"Nature Microbiol","first-page":"1","article-title":"Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis","volume":"1","author":"Limmathurotsakul","year":"2016"},{"key":"ref17","series-title":"J Clin Microbiol","first-page":"2068","article-title":"Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei","volume":"41","author":"Godoy","year":"2003"},{"key":"ref18","series-title":"J Bacteriol","first-page":"1518","article-title":"eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data","volume":"186","author":"Feil","year":"2004"},{"key":"ref19","series-title":"J Clin Microbiol","first-page":"5477","article-title":"Isolates of Burkholderia pseudomallei from northern Australia are distinct by multilocus sequence typing, but strain types do not correlate with clinical presentation","volume":"42","author":"Cheng","year":"2004"},{"key":"ref20","series-title":"J Clin Microbiol","first-page":"249","article-title":"Genetic diversity of Burkholderia pseudomallei isolates in Australia","volume":"46","author":"Cheng","year":"2008"},{"key":"ref21","series-title":"J Clin Microbiol","first-page":"3828","article-title":"Australian and Thai isolates of Burkholderia pseudomallei are distinct by multilocus sequence typing: revision of a case of mistaken identity","volume":"45","author":"Currie","year":"2007"},{"key":"ref22","series-title":"J Clin Microbiol","first-page":"323","article-title":"Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy","volume":"53","author":"De Smet","year":"2015"},{"key":"ref23","series-title":"Appl Environ Microbiol","first-page":"954","article-title":"Unprecedented melioidosis cases in northern Australia caused by an Asian Burkholderia pseudomallei strain identified by using large-scale comparative genomics","volume":"82","author":"Price","year":"2016"}],"container-title":["The American Society of Tropical Medicine and 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