{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:14:46Z","timestamp":1762899286630},"reference-count":21,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,10,5]]},"abstract":"<jats:p>Microscopic examination is the standard method for diagnosis of cutaneous and mucocutaneous leishmaniasis despite its low sensitivity. This study compared the diagnosis efficacy of microscopic examination versus polymerase chain reaction (PCR)\u2013based methods and DNA sequencing using whole blood and skin lesion samples from patients with suspected leishmaniasis. The presence of <jats:italic>Leishmania<\/jats:italic> was determined by microscopy and amplification of 18S ribosomal RNA gene from blood and skin samples of 22 patients. Twenty individuals were positive for leishmaniasis. Microscopic analysis identified 85%, whereas PCR identified 100% of positive cases from skin and 90% from blood. <jats:italic>Cytochrome b<\/jats:italic> gene (<jats:italic>cyt-b<\/jats:italic>) amplification and sequencing identified <jats:italic>Leishmania guyanensis<\/jats:italic>, <jats:italic>Leishmania shawi<\/jats:italic>, and <jats:italic>Leishmania naiffi<\/jats:italic> from skin and blood samples. This study demonstrated the usefulness of whole blood and molecular techniques for the diagnosis and species identification of leishmaniasis.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0385","type":"journal-article","created":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T03:04:45Z","timestamp":1470107085000},"page":"803-805","source":"Crossref","is-referenced-by-count":5,"title":["Diagnostic Efficacy of Molecular Techniques for Detection and Identification of Leishmania Species in Human Whole Blood and Skin Samples from Ecuador"],"prefix":"10.4269","volume":"95","author":[{"given":"Erika B.","family":"Mu\u00f1oz","sequence":"first","affiliation":[{"name":"1Centro de Investigaci\u00f3n Traslacional, Universidad de Las Am\u00e9ricas (CIT-UDLA), Quito, Ecuador."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Santander","sequence":"additional","affiliation":[{"name":"1Centro de Investigaci\u00f3n Traslacional, Universidad de Las Am\u00e9ricas (CIT-UDLA), Quito, Ecuador."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricio","family":"Rojas-Silva","sequence":"additional","affiliation":[{"name":"1Centro de Investigaci\u00f3n Traslacional, Universidad de Las Am\u00e9ricas (CIT-UDLA), Quito, Ecuador."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Cardenas","sequence":"additional","affiliation":[{"name":"1Centro de Investigaci\u00f3n Traslacional, Universidad de Las Am\u00e9ricas (CIT-UDLA), Quito, Ecuador."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Fornasini","sequence":"additional","affiliation":[{"name":"1Centro de Investigaci\u00f3n Traslacional, Universidad de Las Am\u00e9ricas (CIT-UDLA), Quito, Ecuador."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara C.","family":"Cifuentes","sequence":"additional","affiliation":[{"name":"1Centro de Investigaci\u00f3n Traslacional, Universidad de Las Am\u00e9ricas (CIT-UDLA), Quito, Ecuador."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Salvador","sequence":"additional","affiliation":[{"name":"2Instituto Ecuatoriano de Seguridad Social, Quito, Ecuador."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel E.","family":"Balde\u00f3n","sequence":"additional","affiliation":[{"name":"1Centro de Investigaci\u00f3n Traslacional, Universidad de Las Am\u00e9ricas (CIT-UDLA), Quito, Ecuador."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Sci Rep","first-page":"8550","article-title":"The genome of Leishmania panamensis: insights into genomics of the L. 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