{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T06:49:40Z","timestamp":1791442180526,"version":"4.3.3"},"reference-count":18,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,4,5]]},"abstract":"<jats:p>A modification of Koga agar plate culture was performed, consisting of a 2 \u00d7 2-cm cellophane paper centered on the agar plate to prevent bacterial contamination of the agar and daily dish examinations (days 2\u20135). Between January 2000 and July 2005, we examined 1,708 infection-suspected patients, of which 147 (8.6%) harbored <jats:italic>S. stercoralis<\/jats:italic>. Single modified agar plate cultures exhibited superior sensitivity (93.2%), compared with different three-sample screening methods (sensitivity\u2014Baermann: 76.6%, formalin-ethyl acetate: 22%, and direct smear: 15.3%). Agar plate cultures stand out as helpful alternatives for improved detection and therapy monitoring in poor countries and endemic areas. Combined with Baermann methods, they provide increased probability for <jats:italic>S. stercoralis<\/jats:italic> detection.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0414","type":"journal-article","created":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T01:50:28Z","timestamp":1485222628000},"page":"863-865","source":"Crossref","is-referenced-by-count":16,"title":["Improved Detection of Strongyloides stercoralis in Modified Agar Plate Cultures"],"prefix":"10.4269","volume":"96","author":[{"given":"Leonor A.","family":"Pocaterra","sequence":"first","affiliation":[{"name":"C\u00e1tedra de Parasitolog\u00eda, Escuela de Medicina \u201cJos\u00e9 Mar\u00eda Vargas,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Ferrara","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitosis Intestinales, Escuela de Medicina \u201cJos\u00e9 Mar\u00eda Vargas,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaura","family":"Pe\u00f1aranda","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Parasitolog\u00eda, Escuela de Medicina \u201cJos\u00e9 Mar\u00eda Vargas,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsy","family":"Rojas","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitosis Intestinales, Escuela de Medicina \u201cJos\u00e9 Mar\u00eda Vargas,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gladymar","family":"P\u00e9rez-Chac\u00f3n","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Parasitolog\u00eda, Escuela de Medicina \u201cJos\u00e9 Mar\u00eda Vargas,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurora","family":"Hern\u00e1n","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Parasitolog\u00eda, Escuela de Medicina \u201cJos\u00e9 Mar\u00eda Vargas,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Certad","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Parasitolog\u00eda, Escuela de Medicina \u201cJos\u00e9 Mar\u00eda Vargas,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Goldstein","sequence":"additional","affiliation":[{"name":"Medicina Interna-Hematolog\u00eda, Centro M\u00e9dico de Caracas, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz","family":"N\u00fa\u00f1ez","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Parasitolog\u00eda, Escuela de Medicina \u201cJos\u00e9 Mar\u00eda Vargas,\u201d Universidad Central de Venezuela, Caracas, Venezuela."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"PLoS Negl Trop Dis","first-page":"e2288","article-title":"Strongyloides stercoralis: global distribution and risk factors","volume":"7","author":"Sch\u00e4r","year":"2013"},{"key":"ref2","series-title":"Epidemiol Infect","first-page":"452","article-title":"Prevalence of strongyloidiasis in Latin America: a systematic review of the literature","volume":"143","author":"Buonfrate","year":"2015"},{"key":"ref3","series-title":"PLoS Negl Trop Dis","first-page":"e2214","article-title":"Strongyloides stercoralis: a plea for action","volume":"7","author":"Bisoffi","year":"2013"},{"key":"ref4","series-title":"PLoS Negl Trop Dis","first-page":"e2002","article-title":"The laboratory diagnosis and follow up of strongyloidiasis: a systematic 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Microbiol","first-page":"68","article-title":"Comparaci\u00f3n de cuatro t\u00e9cnicas de laboratorio para el diagn\u00f3stico de estrongiloidiasis","volume":"22","author":"Ribero","year":"2002"},{"key":"ref10","series-title":"Diagn Microbiol Infect Dis","first-page":"141","article-title":"Value of the agar plate method for the diagnosis of intestinal strongyloidiasis","volume":"23","author":"Salazar","year":"1995"},{"key":"ref11","series-title":"Rev Esp Salud Publica","first-page":"581","article-title":"Infecci\u00f3n por Strongyloides stercoralis: metan\u00e1lisis sobre evaluaci\u00f3n de m\u00e9todos diagn\u00f3sticos convencionales (1980\u20132013)","volume":"88","author":"Campo-Polanco","year":"2014"},{"key":"ref12","series-title":"Trop Parasitol","first-page":"80","article-title":"Cultivation of parasites","volume":"4","author":"Ahmed","year":"2014"},{"key":"ref13","series-title":"PLoS Negl Trop Dis","first-page":"e2035","article-title":"Diagnosis, treatment and risk factors of Strongyloides stercoralis in schoolchildren in Cambodia","volume":"7","author":"Khieu","year":"2013"},{"key":"ref14","series-title":"PLoS Negl Trop Dis","first-page":"e75","article-title":"Occurrence of Strongyloides stercoralis in Yunnan Province, China, and comparison of diagnostic methods","volume":"1","author":"Steinmann","year":"2007"},{"key":"ref15","series-title":"PLoS Negl Trop Dis","first-page":"e1292","article-title":"Diagnosis, clinical features, and self-reported morbidity of Strongyloides stercoralis and hookworm infection in a co-endemic setting","volume":"5","author":"Becker","year":"2011"},{"key":"ref16","series-title":"Clin Infect Dis","first-page":"1040","article-title":"Diagnosis of Strongyloides stercoralis infection","volume":"33","author":"Siddiqui","year":"2001"},{"key":"ref17","series-title":"PLoS Negl Trop Dis","first-page":"e0004578","article-title":"Improved PCR-based detection of soil transmitted helminth infections using a next-generation sequencing 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