{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T20:23:59Z","timestamp":1789590239976,"version":"build-2803163510"},"reference-count":46,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,9,7]]},"abstract":"<jats:p>Etiology of acute undifferentiated febrile syndrome (AUFS) is often unknown, leading to inaccurate diagnosis and treatment. Villeta town has been identified as an endemic area for spotted fever group (SFG) rickettsioses but little is known about possible amplifier hosts and other <jats:italic>Rickettsia<\/jats:italic> species different from <jats:italic>Rickettsia rickettsii<\/jats:italic>. Besides, few studies have approached other AUFS etiologies in the region. We investigated the role of dengue, leptospirosis, rickettsioses, human anaplasmosis, and Q fever as possible causes of AUFS in patients from Villeta. Sera specimens and ticks from animals as well as ticks from vegetation were studied for the presence of different <jats:italic>Rickettsia<\/jats:italic> spp. Among 104 sera from patients with AUFS, 16.4%, 24.0%, and 2.9% patients seroconverted to dengue, <jats:italic>Leptospira<\/jats:italic>, and SFG <jats:italic>Rickettsia<\/jats:italic>, respectively, with a case of probable coinfection or cross-reaction with <jats:italic>Anaplasma phagocytophilum<\/jats:italic>. None of the samples were reactive for <jats:italic>Coxiella burnetii<\/jats:italic>. Sera samples from 74 horses, 118 dogs, and 62 bovines were collected and showed 33.8%, 14.4%, and 50.0% of seroprevalence for SFG <jats:italic>Rickettsia<\/jats:italic>, respectively. A total of 1,287 ixodid ticks were collected from animals\/vegetation and processed in pools for polymerase chain reaction. Among them, 1.7% was positive for <jats:italic>Rickettsia<\/jats:italic> genes, and <jats:italic>Rickettsia amblyommii<\/jats:italic>, <jats:italic>R. rickettsii<\/jats:italic>, and <jats:italic>Rickettsia<\/jats:italic> spp. were found. These results confirm the circulation of dengue, different SFG <jats:italic>Rickettsia<\/jats:italic> species and the relevance of other etiologies like leptospirosis and human anaplasmosis. Further studies must identify different epidemiological variables to establish proper surveillance and control programs.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0442","type":"journal-article","created":{"date-parts":[[2017,9,8]],"date-time":"2017-09-08T06:03:43Z","timestamp":1504850623000},"page":"782-788","source":"Crossref","is-referenced-by-count":33,"title":["Epidemiology of Spotted Fever Group Rickettsioses and Acute Undifferentiated Febrile Illness in Villeta, Colombia"],"prefix":"10.4269","volume":"97","author":[{"given":"\u00c1lvaro A.","family":"Faccini-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Pontificia Universidad Javeriana, Bogot\u00e1, Colombia;"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Doen\u00e7as Infecciosas, Centro de Ci\u00eancias da Sa\u00fade, Universidade Federal do Esp\u00edrito Santo, Vit\u00f3ria, ES, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Ram\u00edrez-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grupo Parasitolog\u00eda Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogot\u00e1, Colombia;"},{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, SP, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Barreto","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Pontificia Universidad Javeriana, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elkin","family":"Forero-Becerra","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria, Universidade Federal de Vi\u00e7osa, Vi\u00e7osa, MG, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Mill\u00e1n","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Pontificia Universidad Javeriana, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elkin","family":"Valbuena","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Pontificia Universidad Javeriana, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea C.","family":"S\u00e1nchez-Alfonso","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Pontificia Universidad Javeriana, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilson O.","family":"Imbacu\u00e1n-Pantoja","sequence":"additional","affiliation":[{"name":"Grupo Parasitolog\u00eda Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas A.","family":"Cort\u00e9s-Vecino","sequence":"additional","affiliation":[{"name":"Grupo Parasitolog\u00eda Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis J.","family":"Polo-Ter\u00e1n","sequence":"additional","affiliation":[{"name":"Grupo Parasitolog\u00eda Veterinaria, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogot\u00e1, Colombia;"},{"name":"Grupo Salud P\u00fablica Veterinaria, Departamento Salud Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00e9stor","family":"Yaya-Lancheros","sequence":"additional","affiliation":[{"name":"Grupo Salud P\u00fablica Veterinaria, Departamento Salud Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"J\u00e1come","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Pontificia Universidad Javeriana, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M.","family":"Palomar","sequence":"additional","affiliation":[{"name":"Departamento de Enfermedades Infecciosas, Centro de Rickettsiosis y Enfermedades Transmitidas por Artr\u00f3podos Vectores, Hospital San Pedro\u2013CIBIR, Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Santib\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Departamento de Enfermedades Infecciosas, Centro de Rickettsiosis y Enfermedades Transmitidas por Artr\u00f3podos Vectores, Hospital San Pedro\u2013CIBIR, Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ar\u00e1nzazu","family":"Portillo","sequence":"additional","affiliation":[{"name":"Departamento de Enfermedades Infecciosas, Centro de Rickettsiosis y Enfermedades Transmitidas por Artr\u00f3podos Vectores, Hospital San Pedro\u2013CIBIR, Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"Oteo","sequence":"additional","affiliation":[{"name":"Departamento de Enfermedades Infecciosas, Centro de Rickettsiosis y Enfermedades Transmitidas por Artr\u00f3podos Vectores, Hospital San Pedro\u2013CIBIR, Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marylin","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Grupo de Enfermedades Infecciosas, Departamento de Microbiolog\u00eda, Pontificia Universidad Javeriana, Bogot\u00e1, Colombia;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Am J Trop Med Hyg","first-page":"875","article-title":"Undifferentiated febrile illness in Kathmandu, Nepal","volume":"92","author":"Thompson","year":"2015"},{"key":"ref2","article-title":"Bolet\u00edn Epidemiol\u00f3gico Semanal. Semana Epidemiol\u00f3gica N\u00famero 52 de 2015 (11 dic. al 02 ene.)","year":"2015"},{"key":"ref3","series-title":"Am J Trop Med","first-page":"639","article-title":"A spotted fever in Tobia, Colombia","volume":"17","author":"Patino","year":"1937"},{"key":"ref4","series-title":"Bol Of Sanit Panam (Engl)","first-page":"1112","article-title":"Nuevas observaciones sobre un tercer foco de fiebre petequial (maculosa) en el hemisferio americano","volume":"20","author":"Pati\u00f1o-Camargo","year":"1941"},{"key":"ref5","series-title":"Emerg Infect Dis","first-page":"1058","article-title":"Rocky Mountain spotted fever, Colombia","volume":"13","author":"Hidalgo","year":"2007"},{"key":"ref6","series-title":"Am J Trop Med Hyg","first-page":"378","article-title":"Prevalence of antibodies against spotted fever group rickettsiae in a rural area of Colombia","volume":"77","author":"Hidalgo","year":"2007"},{"key":"ref7","series-title":"Am J Trop Med Hyg","first-page":"1029","article-title":"A survey of antibodies against Rickettsia rickettsii and Ehrlichia chafeensis in domestic animals from a rural area of Colombia","volume":"80","author":"Hidalgo","year":"2009"},{"key":"ref8","series-title":"Vet Med Int","first-page":"1","article-title":"Brazilian spotted fever with an approach in veterinary medicine and One Health perspective","volume":"2016","author":"Campos","year":"2016"},{"key":"ref9","series-title":"Front Public Health","first-page":"55","article-title":"Challenges posed by tick-borne rickettsiae: eco-epidemiology and public health implications","volume":"3","author":"Eremeeva","year":"2015"},{"key":"ref10","series-title":"Emerg Infect Dis","first-page":"537","article-title":"Rickettsia rickettsii in Amblyomma patinoi ticks, Colombia","volume":"21","author":"Faccini-Mart\u00ednez","year":"2015"},{"key":"ref11","series-title":"Int J Infect Dis","first-page":"126","article-title":"Syndromic classification of rickettsioses: an approach for clinical practice","volume":"28","author":"Faccini-Mart\u00ednez","year":"2014"},{"key":"ref12","series-title":"Vector Borne Zoonotic Dis","first-page":"289","article-title":"Flea-borne rickettsioses in the North of Caldas Province, Colombia","volume":"13","author":"Hidalgo","year":"2013"},{"key":"ref13","series-title":"Carrapatos de Import\u00e2ncia M\u00e9dico-Veterin\u00e1ria da Regi\u00e3o Neotropical: Um Guia Ilustrado para Identifica\u00e7\u00e3o de Esp\u00e9cies","first-page":"223","author":"Barros-Battesti","year":"2006"},{"key":"ref14","series-title":"Ticks Tick Borne Dis","first-page":"75","article-title":"Nymphs of the genus Amblyomma (Acari: Ixodidae) of Brazil: descriptions, redescriptions, and identification key","volume":"1","author":"Martins","year":"2010"},{"key":"ref15","series-title":"Am J Trop Med Hyg","first-page":"453","article-title":"Molecular detection of Rickettsia felis in different flea species from Caldas, Colombia","volume":"89","author":"Ram\u00edrez-Hern\u00e1ndez","year":"2013"},{"key":"ref16","series-title":"Proc Natl Acad Sci USA","first-page":"10034","article-title":"Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences","volume":"91","author":"Black","year":"1994"},{"key":"ref17","series-title":"J Clin Microbiol","first-page":"2058","article-title":"Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpA","volume":"34","author":"Roux","year":"1996"},{"key":"ref18","series-title":"J Bacteriol","first-page":"1576","article-title":"Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes","volume":"173","author":"Regnery","year":"1991"},{"key":"ref19","series-title":"Emerg Infect Dis","first-page":"237","article-title":"Spotted fever group and typhus group rickettsioses in humans, South Korea","volume":"11","author":"Choi","year":"2005"},{"key":"ref20","series-title":"Biomedica","first-page":"153","article-title":"Comportamiento de la vigilancia epidemiol\u00f3gica de leptospirosis humana en Colombia, 2007\u20132011","volume":"33","author":"Bello","year":"2013"},{"key":"ref21","series-title":"Lancet Infect Dis","first-page":"757","article-title":"Leptospirosis: a zoonotic disease of global importance","volume":"3","author":"Bharti","year":"2003"},{"key":"ref22","series-title":"Biomedica","first-page":"99","article-title":"Etiolog\u00eda y caracterizaci\u00f3n epidemiol\u00f3gica del s\u00edndrome febril no pal\u00fadico en tres municipios del Urab\u00e1 antioque\u00f1o, Colombia","volume":"33","author":"Arroyave","year":"2013"},{"key":"ref23","series-title":"Informe Quincenal Epidemiol\u00f3gico Nacional","first-page":"177","article-title":"Brote de rickettsiosis en Necocl\u00ed, Antioquia, febrero-marzo de 2006","volume":"11","author":"Acosta","year":"2006"},{"key":"ref24","series-title":"Informe Quincenal Epidemiol\u00f3gico Nacional","first-page":"145","article-title":"Estudio de brote febril hemorr\u00e1gico en el corregimiento de Alto de Mulatos: Distrito Especial Portuario de Turbo, Antioquia, enero de 2008","volume":"13","author":"Pacheco-Garc\u00eda","year":"2008"},{"key":"ref25","series-title":"Am J Trop Med Hyg","first-page":"1212","article-title":"Human infections by multiple spotted fever group rickettsiae in Tennessee","volume":"94","author":"Delisle","year":"2016"},{"key":"ref26","series-title":"Rev MVZ Cordoba","first-page":"781","article-title":"Detection of antibodies to Anaplasma, Bartonella, and Coxiella in rural inhabitants of the Caribbean area of Colombia","volume":"11","author":"Mattar","year":"2006"},{"key":"ref27","series-title":"Infect Genet Evol","first-page":"1842","article-title":"Anaplasma, Ehrlichia, and \u201cCandidatus Neorhrlichia\u201d bacteria: pathogenicity, biodiversity, and molecular genetic characteristics, a review","volume":"11","author":"Rar","year":"2011"},{"key":"ref28","series-title":"Exp Appl Acarol","first-page":"83","article-title":"Ticks (Ixodidae) on humans in South America","volume":"40","author":"Guglielmone","year":"2006"},{"key":"ref29","series-title":"Parasit Vectors","first-page":"348","article-title":"Molecular epidemiology of the emerging zoonosis agent Anaplasma phagocytophilum (Foggie, 1949) in dogs and ixodid ticks in Brazil","volume":"6","author":"Santos","year":"2013"},{"key":"ref30","series-title":"Ann Trop Med Parasitol","first-page":"283","article-title":"The distribution and ecological \u2018preferences\u2019 of the tick Amblyomma cajennense (Acari: Ixodidae), an ectoparasite of humans and other mammals","volume":"98","author":"Estrada-Pe\u00f1a","year":"2004"},{"key":"ref31","series-title":"J Wildl Dis","first-page":"153","article-title":"Mammalian wildlife diseases as hazards to man and livestock in an area of the Llanos Orientales of Colombia","volume":"17","author":"Wells","year":"1981"},{"key":"ref32","series-title":"Acta Biolo Colomb","first-page":"153","article-title":"Canis familiaris, Un nuevo hospedero de Ornithodoros (A.) puertoricensis FOX, 1947 (Acari: Ixodida) en Colombia","volume":"14","author":"Paternina","year":"2009"},{"key":"ref33","series-title":"Ticks Tick Borne Dis","first-page":"672","article-title":"Infection of Amblyomma ovale by Rickettsia sp. strain Atlantic rainforest, Colombia","volume":"5","author":"Londo\u00f1o","year":"2014"},{"key":"ref34","series-title":"J Med Entomol","first-page":"960","article-title":"Rickettsia sp. strain Colombianensi (Rickettsiales: Rickettsiaceae): a new proposed Rickettsia detected in Amblyomma dissimile (Acari: Ixodidae) from iguanas and free-living larvae ticks from vegetation","volume":"49","author":"Miranda","year":"2012"},{"key":"ref35","series-title":"Ticks Tick Borne Dis","first-page":"208","article-title":"Molecular detection of Rickettsia bellii and Rickettsia sp. strain Colombianensi in ticks from Cordoba, Colombia","volume":"5","author":"Miranda","year":"2014"},{"key":"ref36","series-title":"Vector Borne Zoonotic Dis","first-page":"85","article-title":"Molecular evidence of different Rickettsia species in Villeta, Colombia","volume":"16","author":"Faccini-Mart\u00ednez","year":"2016"},{"key":"ref37","series-title":"Acta Med Costarric","first-page":"83","article-title":"Detecci\u00f3n molecular de Rickettsia spp. (Da Rocha-Lima, 1916) en garrapatas recolectadas en tres regiones de Colombia","volume":"55","author":"Ram\u00edrez-Hern\u00e1ndez","year":"2013"},{"key":"ref38","series-title":"J Med Entomol","first-page":"856","article-title":"Detection and identification of rickettsial agents in ticks from domestic mammals in eastern Panama","volume":"46","author":"Berm\u00fadez","year":"2009"},{"key":"ref39","series-title":"Infect Genet Evol","first-page":"122","article-title":"Genotyping, evolution and epidemiological findings of Rickettsia species","volume":"25","author":"Merhej","year":"2014"},{"key":"ref40","series-title":"J Med Entomol","first-page":"660","article-title":"Dynamics of exposure to Rickettsia parkeri in cattle in the Paran\u00e1 River Delta, Argentina","volume":"53","author":"Monje","year":"2016"},{"key":"ref41","series-title":"Parasit Vectors","first-page":"46","article-title":"Investigation of tick-borne bacteria (Rickettsia spp., Anaplasma spp., Ehrlichia spp. and Borrelia spp.) in ticks collected from Andean tapirs, cattle and vegetation from a protected area in Ecuador","volume":"8","author":"Pesquera","year":"2015"},{"key":"ref42","series-title":"Vector Borne Zoonotic Dis","first-page":"557","article-title":"\u201cRickettsia amblyommii\u201d induces cross protection against lethal Rocky Mountain spotted fever in a guinea pig model","volume":"14","author":"Blanton","year":"2014"},{"key":"ref43","series-title":"Ticks Tick Borne Dis","first-page":"805","article-title":"Pathogenic potential of a Costa Rican strain of \u2018Candidatus Rickettsia amblyommii\u2019 in guinea pigs (Cavia porcellus) and protective immunity against Rickettsia rickettsii","volume":"6","author":"Rivas","year":"2015"},{"key":"ref44","series-title":"Am J Trop Med Hyg","first-page":"3","article-title":"Changing dynamics of human-rickettsial interactions","volume":"94","author":"Walker","year":"2016"},{"key":"ref45","series-title":"Parasitology","first-page":"1283","article-title":"Epidemiology of Brazilian spotted fever in the Atlantic Forest, state of S\u00e3o Paulo, Brazil","volume":"139","author":"Ogrzewalska","year":"2012"},{"key":"ref46","series-title":"Trends Ecol Evol","first-page":"239","article-title":"Biodiversity and disease: a synthesis of ecological perspectives on Lyme disease transmission","volume":"28","author":"Wood","year":"2013"}],"container-title":["The American Journal of Tropical Medicine and 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