{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T09:41:17Z","timestamp":1772271677357,"version":"3.50.1"},"reference-count":43,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,9,6]]},"abstract":"<jats:p>Multiplex bead assays (MBAs) may provide a powerful integrated tool for monitoring, evaluation, and post-elimination surveillance of onchocerciasis and co-endemic diseases; however, the specificity and sensitivity of <jats:italic>Onchocerca volvulus<\/jats:italic> antigens have not been characterized within this context. An MBA was developed to evaluate three antigens (OV-16, OV-17, and OV-33) for onchocerciasis. Receiver operating characteristics (ROC) analyses were used to characterize antigen performance using a panel of 610 specimens: 109 <jats:italic>O. volvulus<\/jats:italic>-positive specimens, 426 non-onchocerciasis controls with filarial and other confirmed parasitic infection, and 75 sera from patients with no other parasitic infection. The IgG and IgG4 assays for OV-16 demonstrated sensitivities of 95.4% and 96.3%, and specificities of 99.4% and 99.8%, respectively. The OV-17 IgG and IgG4 assays had sensitivities of 86.2% and 76.1% and specificities of 79.2% and 82.8%. For OV-33, the IgG and IgG4 assays had sensitivities of 90.8% and 96.3%, and specificities of 96.8% and 98.6%. The OV-16 IgG4-based MBA had the best assay characteristics, followed by OV-33 IgG4. The OV-16 IgG4 assay would be useful for monitoring and evaluation using the MBA platform. Further evaluations are needed to review the potential use of OV-33 as a confirmatory test in the context of program evaluations.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0519","type":"journal-article","created":{"date-parts":[[2017,7,4]],"date-time":"2017-07-04T07:00:40Z","timestamp":1499151640000},"page":"666-672","source":"Crossref","is-referenced-by-count":12,"title":["Characterizing Reactivity to Onchocerca volvulus Antigens in Multiplex Bead Assays"],"prefix":"10.4269","volume":"97","author":[{"given":"Karla R.","family":"Feeser","sequence":"first","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitaliano","family":"Cama","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey W.","family":"Priest","sequence":"additional","affiliation":[{"name":"Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Thiele","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan E.","family":"Wiegand","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomson","family":"Lakwo","sequence":"additional","affiliation":[{"name":"Vector Control Division, Uganda Ministry of Health, Kampala, Uganda"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sindew M.","family":"Feleke","sequence":"additional","affiliation":[{"name":"Ethiopian Public Health Institute, Addis Ababa, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul T.","family":"Cantey","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Wkly Epidemiol Rec","first-page":"505","article-title":"Progress Report on the Elimination of Human Onchocerciasis, 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