{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T19:29:41Z","timestamp":1784575781283,"version":"3.55.0"},"reference-count":28,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,2,8]]},"abstract":"<jats:p><jats:italic>Fasciola hepatica<\/jats:italic> is the most widely distributed trematode infection in the world. Control efforts may be hindered by the lack of diagnostic capacity especially in remote endemic areas. Polymerase chain reaction (PCR)\u2013based methods offer high sensitivity and specificity but require expensive technology. However, the recombinase polymerase amplification (RPA) is an efficient isothermal method that eliminates the need for a thermal cycler and has a high deployment potential to resource-limited settings. We report on the characterization of RPA and PCR tests to detect <jats:italic>Fasciola<\/jats:italic> infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing no cross-reactivity with trematode, cestode, or nematode parasites. In addition, RPA and PCR were able to detect 47% and 26% of infections not detected by microscopy, respectively. The RPA adapted to a lateral flow platform was more sensitive than gel-based detection of the reaction products. In conclusion, the <jats:italic>Fasciola<\/jats:italic> RPA is a highly sensitive and specific test to diagnose chronic infection using stool samples. The <jats:italic>Fasciola<\/jats:italic> RPA lateral flow has the potential for deployment to endemic areas after further characterization.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0601","type":"journal-article","created":{"date-parts":[[2016,11,8]],"date-time":"2016-11-08T03:44:29Z","timestamp":1478576669000},"page":"341-346","source":"Crossref","is-referenced-by-count":50,"title":["Recombinase Polymerase Amplification Compared to Real-Time Polymerase Chain Reaction Test for the Detection of Fasciola hepatica in Human Stool"],"prefix":"10.4269","volume":"96","author":[{"given":"Miguel M.","family":"Cabada","sequence":"first","affiliation":[{"name":"Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas."},{"name":"Department of Medicine, Alexander von Humboldt Tropical Medicine Institute, Universidad Peruana Cayetano Heredia, Lima, Peru."},{"name":"Universidad Peruana Cayetano Heredia-University of Texas Medical Branch Collaborative Research Center-Cusco, Cusco, Peru."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose L.","family":"Malaga","sequence":"additional","affiliation":[{"name":"Department of Medicine, Alexander von Humboldt Tropical Medicine Institute, Universidad Peruana Cayetano Heredia, Lima, Peru."},{"name":"Universidad Peruana Cayetano Heredia-University of Texas Medical Branch Collaborative Research Center-Cusco, Cusco, Peru."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Castellanos-Gonzalez","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelli A.","family":"Bagwell","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick A.","family":"Naeger","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayley K.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safa","family":"Maharsi","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryann","family":"Mbaka","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Clinton","family":"White","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, Department of Internal Medicine, University of Texas Medical Branch, Galveston, Texas."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Lancet Infect Dis","first-page":"210","article-title":"Global burden of human food-borne trematodiasis: a systematic review and meta-analysis","volume":"12","author":"F\u00fcrst","year":"2012"},{"key":"ref2","series-title":"Trans R Soc Trop Med Hyg","first-page":"480","article-title":"Epidemiology of human fascioliasis and intestinal parasitosis among schoolchildren in Lake Tana Basin, northwest Ethiopia","volume":"107","author":"Fentie","year":"2013"},{"key":"ref3","series-title":"Zoonotic Fasciolosis in Vietnam: Molecular Identification and Geographical Distribution","author":"Nguyen","year":"2012"},{"key":"ref4","series-title":"Parasitol Res","first-page":"61","article-title":"Human 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