{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T18:04:43Z","timestamp":1771265083583,"version":"3.50.1"},"reference-count":18,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,3,8]]},"abstract":"<jats:p><jats:italic>Leishmania<\/jats:italic> (<jats:italic>Viannia<\/jats:italic>) <jats:italic>braziliensis<\/jats:italic> is the main causative species of tegumentary leishmaniasis in Brazil. In this study, we evaluated the susceptibility of 16 clinical isolates of <jats:italic>L.<\/jats:italic> (<jats:italic>V.<\/jats:italic>) <jats:italic>braziliensis<\/jats:italic> from different regions of Brazil to miltefosine in vitro. Half-maximal inhibitory concentrations of miltefosine varied from 22.9 to 144.2 \u03bcM against promastigotes and from 0.3 to 4.2 \u03bcM against intracellular amastigotes. No significant differences were found between isolates of different geographical origins. A clear correlation between the EC<jats:sub>50<\/jats:sub> against promastigotes and amastigotes within each isolate was found. These findings contribute to the evaluation of miltefosine's potential and limitations for the treatment of tegumentary leishmaniasis in Brazil.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0811","type":"journal-article","created":{"date-parts":[[2017,1,10]],"date-time":"2017-01-10T02:20:35Z","timestamp":1484014835000},"page":"656-659","source":"Crossref","is-referenced-by-count":19,"title":["Susceptibility to Miltefosine in Brazilian Clinical Isolates of Leishmania (Viannia) braziliensis"],"prefix":"10.4269","volume":"96","author":[{"given":"Caroline R.","family":"Espada","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Leishmanioses, Departamento de Parasitologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatima","family":"Ribeiro-Dias","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunobiologia das Leishmanioses, Instituto de Patologia Tropical e Sa\u00fade P\u00fablica, Universidade Federal de Goi\u00e1s, Goi\u00e2nia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam L.","family":"Dorta","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunobiologia das Leishmanioses, Instituto de Patologia Tropical e Sa\u00fade P\u00fablica, Universidade Federal de Goi\u00e1s, Goi\u00e2nia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ledice In\u00e1cia de Ara\u00fajo","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunobiologia das Leishmanioses, Instituto de Patologia Tropical e Sa\u00fade P\u00fablica, Universidade Federal de Goi\u00e1s, Goi\u00e2nia, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar M. de","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Imunologia, Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil."},{"name":"Centro de Pesquisas Gon\u00e7alo Moniz, Fiocruz-Bahia, Salvador, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo R.","family":"Machado","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Imunologia, Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Schriefer","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Imunologia, Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenicer K. U.","family":"Yokoyama-Yasunaka","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Leishmanioses, Departamento de Parasitologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriano C.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Leishmanioses, Departamento de Parasitologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil."},{"name":"Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia R. B.","family":"Uliana","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Leishmanioses, Departamento de Parasitologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"PLoS Negl Trop Dis","first-page":"e912","article-title":"Miltefosine in the treatment of cutaneous leishmaniasis caused by Leishmania braziliensis in Brazil: a randomized and controlled trial","volume":"4","author":"Machado","year":"2010"},{"key":"ref2","series-title":"N Engl J Med","first-page":"1739","article-title":"Oral miltefosine for Indian visceral leishmaniasis","volume":"347","author":"Sundar","year":"2002"},{"key":"ref3","series-title":"Clin Infect Dis","first-page":"350","article-title":"Treatment of Bolivian mucosal leishmaniasis with miltefosine","volume":"44","author":"Soto","year":"2007"},{"key":"ref4","series-title":"Clin Infect Dis","first-page":"1266","article-title":"Miltefosine for new world cutaneous leishmaniasis","volume":"38","author":"Soto","year":"2004"},{"key":"ref5","series-title":"Am J Trop Med Hyg","first-page":"255","article-title":"Randomized controlled clinical trial to access efficacy and safety of miltefosine in the treatment of cutaneous leishmaniasis caused by Leishmania (Viannia) guyanensis in Manaus, Brazil","volume":"84","author":"Chrusciak-Talhari","year":"2011"},{"key":"ref6","series-title":"Rev Inst Med Trop Sao Paulo","first-page":"83","article-title":"Leishmania spp. parasite isolation through inoculation of patient biopsy macerates in interferon gamma knockout mice","volume":"52","author":"Oliveira","year":"2010"},{"key":"ref7","series-title":"Mol Biochem Parasitol","first-page":"145","article-title":"Intergenic region typing (IRT): a rapid molecular approach to the characterization and evolution of Leishmania","volume":"73","author":"Cupolillo","year":"1995"},{"key":"ref8","series-title":"Eur J Clin Microbiol Infect Dis","first-page":"1453","article-title":"Three new sensitive and specific heat-shock protein 70 PCRs for global Leishmania species identification","volume":"31","author":"Montalvo","year":"2012"},{"key":"ref9","series-title":"Trop Med Int Health","first-page":"68","article-title":"In vitro sensitivity of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis Brazilian isolates to meglumine antimoniate and amphotericin B","volume":"15","author":"Zauli-Nascimento","year":"2010"},{"key":"ref10","series-title":"Antimicrob Agents Chemother","first-page":"835","article-title":"In vitro susceptibility of field isolates of Leishmania donovani to miltefosine and amphotericin B: correlation with sodium antimony gluconate susceptibility and implications for treatment in areas of endemicity","volume":"53","author":"Kumar","year":"2009"},{"key":"ref11","series-title":"Am J Trop Med Hyg","first-page":"272","article-title":"The sensitivity of clinical isolates of Leishmania from Peru and Nepal to miltefosine","volume":"73","author":"Yardley","year":"2005"},{"key":"ref12","series-title":"PLoS Negl Trop Dis","first-page":"e1657","article-title":"Drug susceptibility in Leishmania isolates following miltefosine treatment in cases of visceral leishmaniasis and post kala-azar dermal leishmaniasis","volume":"6","author":"Bhandari","year":"2012"},{"key":"ref13","series-title":"Am J Trop Med Hyg","first-page":"655","article-title":"In vitro antileishmanial drug susceptibility of clinical isolates from patients with Indian visceral leishmaniasis\u2014status of newly introduced drugs","volume":"87","author":"Prajapati","year":"2012"},{"key":"ref14","series-title":"Am J Trop Med Hyg","first-page":"750","article-title":"In vitro susceptibility of Leishmania donovani to miltefosine in Indian visceral leishmaniasis","volume":"89","author":"Prajapati","year":"2013"},{"key":"ref15","series-title":"Antimicrob Agents Chemother","first-page":"144","article-title":"Treatment failure and miltefosine susceptibility in dermal leishmaniasis caused by Leishmania subgenus Viannia species","volume":"58","author":"Obonaga","year":"2014"},{"key":"ref16","series-title":"J Antimicrob Chemother","first-page":"3023","article-title":"Lack of correlation between the promastigote back-transformation assay and miltefosine treatment outcome","volume":"70","author":"Hendrickx","year":"2015"},{"issue":"(Suppl 1)","key":"ref17","series-title":"Trans R Soc Trop Med Hyg","first-page":"S34","article-title":"Treatment of New World cutaneous leishmaniasis with miltefosine","volume":"100","author":"Soto","year":"2006"},{"key":"ref18","series-title":"J Infect Dis","first-page":"684","article-title":"Noninferiority of miltefosine versus meglumine antimoniate for cutaneous leishmaniasis in children","volume":"205","author":"Rubiano","year":"2012"}],"container-title":["The American Society of Tropical Medicine and 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