{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T22:40:10Z","timestamp":1723070410447},"reference-count":19,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,7,12]]},"abstract":"<jats:p>In Tunisia, chronic cutaneous leishmaniasis due to <jats:italic>Leishmania tropica<\/jats:italic> is an important health problem. Its spreading has not been fully elucidated. Information on sandfly vectors, as well as their associated <jats:italic>Leishmania<\/jats:italic> species, is of paramount importance since vector dispersion is one of the major factors responsible for pathogen dissemination. Ninety-seven unfed females belonging to the genera <jats:italic>Sergentomyia<\/jats:italic> and <jats:italic>Phlebotomus<\/jats:italic> were collected between June and August 2015 using sticky paper traps. Polymerase chain reaction\u2013restriction fragment length polymorphism analysis of the internal transcribed spacer 1and sequencing were used for <jats:italic>Leishmania<\/jats:italic> detection and identification. In total, 650 sandflies were captured and identified (380 males and 270 females). Ninety-seven unfed females were tested for the presence of <jats:italic>Leishmania<\/jats:italic> parasite DNA. Six <jats:italic>Phlebotomus sergenti<\/jats:italic> were found positive for <jats:italic>L. tropica<\/jats:italic>. This novel finding enhances the understanding of the cycle extension of <jats:italic>L. tropica<\/jats:italic> outside its original focus of Tataouine.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0849","type":"journal-article","created":{"date-parts":[[2017,6,6]],"date-time":"2017-06-06T07:00:47Z","timestamp":1496732447000},"page":"291-294","source":"Crossref","is-referenced-by-count":2,"title":["First Report on Natural Infection of Phlebotomus sergenti with Leishmania tropica in a Classical Focus of Leishmania major in Tunisia"],"prefix":"10.4269","volume":"97","author":[{"given":"Kaouther","family":"Jaouadi","sequence":"first","affiliation":[{"name":"Laboratory of Medical Epidemiology, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia; University Tunis El Manar, Tunisia"},{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), LR11IPT02, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jihene","family":"Bettaieb","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Epidemiology, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia; University Tunis El Manar, Tunisia"},{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), LR11IPT02, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia"},{"name":"Faculty of Medicine of Tunis, University Tunis El Manar, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amira","family":"Bennour","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Epidemiology, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia; University Tunis El Manar, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadok","family":"Salem","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Epidemiology, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia; University Tunis El Manar, Tunisia"},{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), LR11IPT02, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Ridha","family":"Rjeibi","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie, Ecole Nationale de M\u00e9decine V\u00e9t\u00e9rinaire de Sidi Thabet, Sidi Thabet, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sana","family":"Chaabane","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Epidemiology, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia; University Tunis El Manar, Tunisia"},{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), LR11IPT02, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rihab","family":"Yazidi","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Epidemiology, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia; University Tunis El Manar, Tunisia"},{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), LR11IPT02, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neila","family":"Khabouchi","sequence":"additional","affiliation":[{"name":"Pole G\u00e9nomique-Plateforme Technique Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adel","family":"Gharbi","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Epidemiology, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia; University Tunis El Manar, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afif Ben","family":"Salah","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Epidemiology, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia; University Tunis El Manar, Tunisia"},{"name":"Laboratory of Transmission, Control and Immunobiology of Infections (LTCII), LR11IPT02, Institut Pasteur de Tunis (IPT), Tunis-Belv\u00e9d\u00e8re, Tunisia"},{"name":"Faculty of Medicine of Tunis, University Tunis El Manar, Tunisia"},{"name":"Department of Family and Community Medicine, College of Medicine and Medical Sciences (CMMS), Arabian Gulf University (AGU), Manama, Bahra\u00efn"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"ref1","series-title":"Bull Soc Pathol Exot","first-page":"12","article-title":"Infantile visceral leishmaniasis from Leishmania infantum MON-24: a reality in Tunisia [in French]","volume":"93","author":"Belhadj","year":"1990"},{"key":"ref2","series-title":"Archives M\u00e9dicales et Pharmaceutiques Militaires","first-page":"296","article-title":"Du bouton de Gafsa au camp de Sathonay","volume":"3","author":"Deperet","year":"1884"},{"year":"1986a","author":"Rioux","key":"ref3"},{"key":"ref4","series-title":"Am J Trop Med Hyg","first-page":"962","article-title":"Natural infection of North African gundi (Ctenodactylus gundi) by Leishmania tropica in the focus of cutaneous leishmaniasis, southeast Tunisia","volume":"86","author":"Bousslimi","year":"2012"},{"key":"ref5","series-title":"Parasit Vectors","first-page":"154","article-title":"First detection of Leishmania killicki (Kinetoplastida, Trypanosomatidae) in Ctenodactylus gundi (Rodentia, Ctenodactylidae), a possible reservoir of human cutaneous leishmaniasis in Tunisia","volume":"11","author":"Jaouadi","year":"2011"},{"key":"ref6","series-title":"Ann Dermatol Venereol","first-page":"419","article-title":"Leishmania tropica cutaneous leishmaniasis in an emerging focus in north Morocco: new clinical forms [in French]","volume":"126","author":"Chiheb","year":"1999"},{"key":"ref7","series-title":"Acta Trop","first-page":"276","article-title":"Twenty-four new human cases of cutaneous leishmaniasis due to Leishmania killicki in Metlaoui, southwestern Tunisia. Probable role of Phlebotomus sergenti in the transmission","volume":"122","author":"Jaouadi","year":"2012"},{"key":"ref8","series-title":"Am J Trop Med Hyg","first-page":"646","article-title":"First report on natural infection of Phlebotomus sergenti with Leishmania promastigotes in the cutaneous leishmaniasis focus in southeastern Tunisia","volume":"85","author":"Tabbabi","year":"2011"},{"key":"ref9","series-title":"Bull Soc Pathol Exot","first-page":"613","article-title":"Natural infestation of Phlebotomus papatasi in a focus of zoonotic cutaneous leishmaniasis in Tunisia [in French]","volume":"80","author":"Ben-Ismail","year":"1986"},{"key":"ref10","series-title":"Trans R Soc Trop Med Hyg","first-page":"499","article-title":"Anthroponotic cutaneous leishmaniasis in Tunisia: presence of Leishmania killicki outside its original focus of Tataouine","volume":"99","author":"Haouas","year":"2005"},{"key":"ref11","series-title":"Ann Parasitol Hum Comp","first-page":"711","article-title":"Phlebotomes de Tunisie (Diptera, Phlebotomidae). Mise au point systematique, chorologique et ethologique","volume":"53","author":"Croset","year":"1978"},{"key":"ref12","series-title":"Med Vet Entomol","first-page":"25","article-title":"Sandflies of the Phlebotomus perniciosus complex: mitochondrial introgression and a new sibling species of P. longicuspis in the Moroccan Rif","volume":"18","author":"Pesson","year":"2004"},{"key":"ref13","series-title":"Diagn Microbiol Infect Dis","first-page":"349","article-title":"PCR diagnosis and characterization of Leishmania in local and imported clinical samples","volume":"47","author":"Sch\u00f6nian","year":"2003"},{"key":"ref14","series-title":"J Mol Biol","first-page":"403","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990"},{"key":"ref15","series-title":"Mol Biol Evol","first-page":"2725","article-title":"MEGA6: molecular evolutionary genetics analysis version 6.0.","volume":"30","author":"Tamura","year":"2013"},{"key":"ref16","series-title":"Em Infect Dis","first-page":"1358","article-title":"Molecular typing reveals emergence of a new clinic-epidemiologic profile of cutaneous leishmanisis in Morocco","volume":"3","author":"Rhajaoui","year":"2007"},{"key":"ref17","series-title":"Acta Trop","first-page":"94","article-title":"Coexistence of Leishmania tropica and Leishmania infantum in Sefrou Province, Morocco","volume":"130","author":"Hmamouch","year":"2014"},{"key":"ref18","series-title":"Trans R Soc Trop Med Hyg","first-page":"382","article-title":"Investigation of the spread of human visceral leishmaniasis in central Tunisia","volume":"94","author":"Ben Salah","year":"2000"},{"key":"ref19","series-title":"Int J Environ Res Public Health","first-page":"329","article-title":"Changes of sand fly populations and Leishmania infantum infection rates in an irrigated village located in arid central Tunisia","volume":"13","author":"Barhoumi","year":"2016"}],"container-title":["The American Society of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/97\/1\/article-p291.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/97\/1\/article-p291.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/97\/1\/article-p291.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T22:23:56Z","timestamp":1723069436000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/97\/1\/article-p291.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,12]]},"references-count":19,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.16-0849","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"type":"print","value":"0002-9637"},{"type":"electronic","value":"1476-1645"}],"subject":[],"published":{"date-parts":[[2017,7,12]]}}}