{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T04:47:36Z","timestamp":1777351656128,"version":"3.51.4"},"reference-count":38,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,10]]},"abstract":"<jats:p>Visceral leishmaniasis (VL) caused by <jats:italic>Leishmania infantum<\/jats:italic> is a lethal disease transmitted by sand flies. Although, considered a zoonosis with dogs held as the main reservoirs, humans are also sources of infection. Therefore, control policies currently focused on dog culling may need to consider that VL and human immunodeficiency virus (HIV)\/VL patients may also be infectious, contributing to transmission. Reservoir competence of patients with VL without and with HIV infection and of persons asymptomatically infected with <jats:italic>Leishmania<\/jats:italic> was assessed by xenodiagnosis with the vector <jats:italic>Lutzomyia longipalpis<\/jats:italic>. Parasites in sand fly\u2019s guts were identified by using optical microscopy and by conventional polymerase chain reaction (PCR). <jats:italic>Leishmania infantum<\/jats:italic> blood parasite burden was determined by quantitative PCR. Among the 61 participants, 27 (44%) infected sand flies as seen by microscopy or PCR. When infectiousness was assessed by microscopy, xenodiagnosis was positive in five (25%) patients not infected with HIV, whereas nine (45%) of those harboring HIV were positive. Among the 19 asymptomatic patients four (21%) infected sand flies only demonstrated by PCR. One (50%) asymptomatic patient with HIV had a positive xenodiagnosis by microscopy. 9\/372 (2.4%) and 37\/398 (9.2%) sand flies were infected when feeding in patients without and with HIV, respectively. Infectiousness was poorly correlated with quantitative PCR. The study shows that asymptomatic humans are capable of transmitting <jats:italic>L. infantum<\/jats:italic>, that ill persons with HIV infection are more infectious to sand flies, and that humans are more important reservoirs than previously thought. This fact may be considered when designing control policies for zoonotic VL.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0883","type":"journal-article","created":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T10:18:21Z","timestamp":1510913901000},"page":"126-133","source":"Crossref","is-referenced-by-count":33,"title":["Human Competence to Transmit Leishmania infantum to Lutzomyia longipalpis and the Influence of Human Immunodeficiency Virus Infection"],"prefix":"10.4269","volume":"98","author":[{"given":"Gabriel Reis","family":"Ferreira","sequence":"first","affiliation":[{"name":"Graduation Program in Sciences and Health, Federal University of Piau\u00ed, Teresina, Brazil;"},{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Carlos","family":"Castelo Branco Ribeiro","sequence":"additional","affiliation":[{"name":"Graduation Program in Sciences and Health, Federal University of Piau\u00ed, Teresina, Brazil;"},{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ant\u00f4nio","family":"Meneses Filho","sequence":"additional","affiliation":[{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresinha","family":"de Jesus Cardoso Farias Pereira","sequence":"additional","affiliation":[{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela Moura","family":"Parente","sequence":"additional","affiliation":[{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humberto Feitosa","family":"Pereira","sequence":"additional","affiliation":[{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jailthon","family":"Carlos da Silva","sequence":"additional","affiliation":[{"name":"Graduation Program in Sciences and Health, Federal University of Piau\u00ed, Teresina, Brazil;"},{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle Alves","family":"Zacarias","sequence":"additional","affiliation":[{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Letiano","family":"Vieira da Silva","sequence":"additional","affiliation":[{"name":"Piaui State Central Laboratory of Public Health, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Symonara Karina","family":"Medeiros Faustino","sequence":"additional","affiliation":[{"name":"Piaui State Central Laboratory of Public Health, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walfrido Salmito","family":"Almeida Neto","sequence":"additional","affiliation":[{"name":"Department of Community Medicine, Federal University of Piau\u00ed, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorcas Lamounier","family":"Costa","sequence":"additional","affiliation":[{"name":"Graduation Program in Sciences and Health, Federal University of Piau\u00ed, Teresina, Brazil;"},{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"},{"name":"Maternal and Child Department, Federal University of Piau\u00ed, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivete","family":"Lopes de Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Animal Sanity Laboratory, Federal University of Piau\u00ed, Teresina, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Henrique","family":"Nery Costa","sequence":"additional","affiliation":[{"name":"Graduation Program in Sciences and Health, Federal University of Piau\u00ed, Teresina, Brazil;"},{"name":"Leishmaniasis Research Laboratory at Natan Portella Tropical Diseases Institute, Teresina, Brazil;"},{"name":"Department of Community Medicine, Federal University of Piau\u00ed, Teresina, Brazil;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"PLoS One","first-page":"e35671","article-title":"Leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"Alvar","year":"2012"},{"key":"b2","series-title":"J Med 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