{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:57:08Z","timestamp":1760889428529},"reference-count":64,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,9,7]]},"abstract":"<jats:p>Scabies is a human skin disease due to the burrowing ectoparasite <jats:italic>Sarcoptes scabiei<\/jats:italic> var. <jats:italic>hominis<\/jats:italic> resulting in intense itching and inflammation and manifesting as a skin allergy. Because of insufficient mite material and lack of in vitro propagation system for antigen preparation, scabies is a challenging disease to develop serological diagnostics. For allergen characterization, full-length <jats:italic>S. scabiei<\/jats:italic> tropomyosin (Sar s 10) was cloned, expressed in pET-15b, and assessed for reactivity with IgE antibodies from human sera. IgE binding was observed to Sar s 10 with sera collected from subjects with ordinary scabies, house dust mite (HDM)-positive and naive subjects and a diagnostic sensitivity of &lt; 30% was observed. <jats:italic>S. scabiei<\/jats:italic> paramyosin (Sar s 11) was cloned, and expressed in pET-28a in three overlapping fragments designated Sspara1, Sspara2, and Sspara3. IgE and IgG binding was observed to Sspara2 and Sspara3 antigens with sera collected from ordinary scabies, and HDM-positive subjects, but no binding was observed with sera collected from naive subjects. Sspara2 displayed excellent diagnostic potential with 98% sensitivity and 90% specificity observed for IgE binding and 70% sensitivity for IgG. In contrast, the diagnostic sensitivity of Sspara3 was 84% for IgE binding and 40% for IgG binding. In combination, Sspara2 and Sspara3 provided an IgE sensitivity of 94%. This study shows that IgE binding to Sspara2 and Sspara3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice. The developed enzyme-linked immunosorbent assay helps direct future development of a specific diagnostic tool for scabies.<\/jats:p>","DOI":"10.4269\/ajtmh.16-0976","type":"journal-article","created":{"date-parts":[[2017,7,11]],"date-time":"2017-07-11T07:00:47Z","timestamp":1499756447000},"page":"851-860","source":"Crossref","is-referenced-by-count":13,"title":["Characterization of Sarcoptes scabiei Tropomyosin and Paramyosin: Immunoreactive Allergens in Scabies"],"prefix":"10.4269","volume":"97","author":[{"given":"Shumaila","family":"Naz","sequence":"first","affiliation":[{"name":"Department of Zoology, Faculty of Sciences, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Desclozeaux","sequence":"additional","affiliation":[{"name":"Inflammation and Healing Research Cluster, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate E.","family":"Mounsey","sequence":"additional","affiliation":[{"name":"Inflammation and Healing Research Cluster, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhana Riaz","family":"Chaudhry","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Sciences, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley F.","family":"Walton","sequence":"additional","affiliation":[{"name":"Inflammation and Healing Research Cluster, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, Queensland, 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