{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:19:06Z","timestamp":1792523946077,"version":"4.5.0"},"reference-count":25,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,12,6]]},"abstract":"<jats:p>Secondary syphilis (SS) has always been puzzling for the clinicians because of the similarity of the appearance of skin rashes with other dermatoses. Serological assays are useful, but less sensitive at an early stage of SS or when patients are immunodeficient. Therefore, there is an urgent need to develop a rapid and effective tool for the diagnosis of SS, which may play an important role in the control of epidemic syphilis outbreaks. In this study, we evaluated a loop-mediated isothermal amplification (LAMP) assay, targeting gene encoding the basic membrane protein of <jats:italic>Treponema pallidum<\/jats:italic>, to detect the presence of circulating <jats:italic>T. pallidum<\/jats:italic> DNA in the blood of SS patients. The specificity of LAMP was validated using three strains of Spirochaetales and six common clinical bacteria. The clinical applicability of LAMP assay was assessed using 642 blood samples from clinically suspected SS patients and 80 samples from healthy blood donors, showing a sensitivity of 82.1% and a specificity of 100.0% in the diagnosis of SS. Thus, our results indicate that the LAMP can be used as a supplementary method for the diagnosis of SS.<\/jats:p>","DOI":"10.4269\/ajtmh.17-0051","type":"journal-article","created":{"date-parts":[[2017,10,3]],"date-time":"2017-10-03T07:00:52Z","timestamp":1507014052000},"page":"1673-1678","source":"Crossref","is-referenced-by-count":19,"title":["Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for the Detection of Treponema pallidum DNA in the Peripheral Blood of Secondary Syphilis Patients"],"prefix":"10.4269","volume":"97","author":[{"given":"Yongjian","family":"Xiao","sequence":"first","affiliation":[{"name":"Institute of Pathogenic Biology Institute, Medical College, University of South China, Hengyang, China;"},{"name":"Hunan Province Cooperative Innovation Center for Molecular Targeted New Drug Study, Hengyang, China;"},{"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of University of South China, Hengyang, China;"},{"name":"Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control, Hengyang, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafeng","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology Institute, Medical College, University of South China, Hengyang, China;"},{"name":"Hunan Province Cooperative Innovation Center for Molecular Targeted New Drug Study, Hengyang, China;"},{"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of University of South China, Hengyang, China;"},{"name":"Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control, Hengyang, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology Institute, Medical College, University of South China, Hengyang, China;"},{"name":"Hunan Province Cooperative Innovation Center for Molecular Targeted New Drug Study, Hengyang, China;"},{"name":"Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control, Hengyang, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of University of South China, Hengyang, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanhao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology Institute, Medical College, University of South China, Hengyang, China;"},{"name":"Hunan Province Cooperative Innovation Center for Molecular Targeted New Drug Study, Hengyang, China;"},{"name":"Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control, Hengyang, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feijun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology Institute, Medical College, University of South China, Hengyang, China;"},{"name":"Hunan Province Cooperative Innovation Center for Molecular Targeted New Drug Study, Hengyang, China;"},{"name":"Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control, Hengyang, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiebing","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology Institute, Medical College, University of South China, Hengyang, China;"},{"name":"Hunan Province Cooperative Innovation Center for Molecular Targeted New Drug Study, Hengyang, China;"},{"name":"Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control, Hengyang, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoran","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of University of South China, Hengyang, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Experimental Zoology, Medical College, University of South China, Hengyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimou","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Pathogenic Biology Institute, Medical College, University of South China, Hengyang, China;"},{"name":"Hunan Province Cooperative Innovation Center for Molecular Targeted New Drug Study, Hengyang, China;"},{"name":"Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control, Hengyang, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Int J Dermatol","first-page":"1434","article-title":"The great imitator revisited: the spectrum of atypical cutaneous manifestations of secondary syphilis","volume":"53","author":"Balagula","year":"2014"},{"key":"b2","series-title":"Int J STD AIDS","first-page":"300","article-title":"IUSTI: 2008 European guidelines on the management of syphilis","volume":"20","author":"French","year":"2009"},{"key":"b3","series-title":"Diagn Microbiol Infect Dis","first-page":"163","article-title":"Amplification of the DNA polymerase I gene of Treponema pallidum from whole blood of persons with syphilis","volume":"40","author":"Marfin","year":"2001"},{"key":"b4","series-title":"J Clin Microbiol","first-page":"546","article-title":"Evaluation of a PCR test for detection of Treponema pallidum in swabs and 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