{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T05:59:46Z","timestamp":1793339986650,"version":"4.5.0"},"reference-count":13,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,11,8]]},"abstract":"<jats:p>In this study, we developed a recombinase polymerase amplification (RPA) assay for specific diagnosis of <jats:italic>Plasmodium knowlesi<\/jats:italic>. Genomic DNA was extracted from whole blood samples using a commercial kit. With incubation at 37\u00b0C, the samples were successfully amplified within 20 minutes. The end product of RPA was further examined by loading onto agarose gel and a specific band was observed with a size of 128 bp. The RPA assay exhibited high sensitivity with limits of detection down to one copy of the plasmid. From the specificity experiments, it was demonstrated that all <jats:italic>P. knowlesi<\/jats:italic> samples (<jats:italic>N<\/jats:italic> = 45) were positive while other <jats:italic>Plasmodium<\/jats:italic> spp. (<jats:italic>N<\/jats:italic> = 42) and negative samples (<jats:italic>N<\/jats:italic> = 6) were negative. Therefore, the RPA assay is a highly promising approach with the potential to be used in resource-limited settings. This assay can be further optimized for bedside and on field application.<\/jats:p>","DOI":"10.4269\/ajtmh.17-0427","type":"journal-article","created":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T07:00:29Z","timestamp":1502780429000},"page":"1597-1599","source":"Crossref","is-referenced-by-count":18,"title":["Rapid Detection of Plasmodium knowlesi by Isothermal Recombinase Polymerase Amplification Assay"],"prefix":"10.4269","volume":"97","author":[{"given":"Meng-Yee","family":"Lai","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Choo-Huck","family":"Ooi","sequence":"additional","affiliation":[{"name":"Sarawak State Health Department, Jalan Diplomatik, Off Jalan Bako, Kuching, Sarawak, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee-Ling","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","author":"World Health Organization","year":"2016"},{"key":"b2","series-title":"Lancet","first-page":"1017","article-title":"A large focus of naturally acquired Plasmodium knowlesi infections in human beings","volume":"363","author":"Singh","year":"2004"},{"key":"b3","series-title":"Malar J","first-page":"73","article-title":"Morphological features and differential counts of Plasmodium knowlesi parasites in naturally acquired human infections","volume":"8","author":"Lee","year":"2009"},{"key":"b4","series-title":"J Clin Microbiol","first-page":"4173","article-title":"Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers 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Biosyst","first-page":"970","article-title":"Isothermal amplified detection of DNA and RNA","volume":"10","author":"Yan","year":"2014"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/97\/5\/article-p1597.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/97\/5\/article-p1597.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/97\/5\/article-p1597.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T16:50:48Z","timestamp":1722444648000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/97\/5\/article-p1597.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,8]]},"references-count":13,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.17-0427","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,8]]}}}