{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:52:41Z","timestamp":1789937561862,"version":"4.0.1"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,2,7]]},"abstract":"<jats:p>Current guidelines for the diagnosis of neurocysticercosis (NCC) recommend the use of the lentil lectin-bound glycoprotein enzyme-linked immunoelectrotransfer blot assay (LLGP-EITB) as the reference standard for serological testing. In response to the drawbacks involved with the use of the LLGP-EITB, a recombinant T24H antigen (rT24H) EITB assay was developed, with promising results. However, the test has yet to be evaluated among individuals from sub-Saharan Africa (SSA). The aim of the present study was to investigate the performance of the rT24H EITB assay for the detection of NCC cases in a panel of serum samples (<jats:italic>N<\/jats:italic> = 366, of which 173 patients presented with epileptic seizures and\/or severe chronic headaches, and 193 matched manifestation-free participants) collected as part of a large community-based trial in Burkina Faso. A perfect agreement between the rT24H EITB and the native gp24 (and its homodimer, gp42) LLGP-EITB was found (kappa value of 1.0). Furthermore, among patients with the neurological manifestations of interest who underwent a computed tomography scan, the rT24H EITB and native antigen LLGP-EITB had a comparable ability to correctly identify NCC cases with multiple viable (rT24H: sensitivity: 80.0%), single viable (66.7%), and calcified\/degenerating cysts only (25.0%), albeit for multiple viable and calcified cysts, the rT24H estimated sensitivity seemed lower, but more uncertain, than previously reported. The rT24H EITB specificity was high (98.2%) and in line with previous studies. This study confirms the value of the recombinant rT24H EITB as an alternative to the native antigen LLGP-EITB for the diagnosis of NCC in a SSA community setting.<\/jats:p>","DOI":"10.4269\/ajtmh.17-0541","type":"journal-article","created":{"date-parts":[[2017,12,27]],"date-time":"2017-12-27T07:00:28Z","timestamp":1514358028000},"page":"565-569","source":"Crossref","is-referenced-by-count":18,"title":["Evaluating the Recombinant T24H Enzyme-Linked Immunoelectrotransfer Blot Assay for the Diagnosis of Neurocysticercosis in a Panel of Samples from a Large Community-Based Randomized Control Trial in 60 Villages in Burkina Faso"],"prefix":"10.4269","volume":"98","author":[{"given":"Veronique","family":"Dermauw","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, 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France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Paule","family":"Boncoeur-Martel","sequence":"additional","affiliation":[{"name":"H\u00f4pital Universitaire Dupuytren, Limoges, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea Sylvia","family":"Winkler","sequence":"additional","affiliation":[{"name":"Department of Neurology, Center for Global Health, Technical University Munich, Munich, Germany;"},{"name":"Department of Community Medicine and Global Health, Centre for Global Health, Institute of Health and Society, University of Oslo, Oslo, Norway;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke","family":"Van Hul","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Dorny","sequence":"additional","affiliation":[{"name":"Department of 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