{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:17:12Z","timestamp":1770221832704,"version":"3.49.0"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,10,3]]},"abstract":"<jats:p><jats:italic>Bartonella rochalimae<\/jats:italic> is an emerging zoonotic pathogen present in the United States, South America, and Europe. The molecular detection of <jats:italic>B. rochalimae<\/jats:italic> frequently relies on polymerase chain reaction (PCR) assays that target the genus <jats:italic>Bartonella<\/jats:italic> coupled with DNA sequencing for species determination. However, the presence of other <jats:italic>Bartonella<\/jats:italic> spp. in the sample being tested may result in false-negative results for <jats:italic>B. rochalimae<\/jats:italic>, especially when Sanger sequencing is used. We developed a sensitive and specific quantitative PCR platform for <jats:italic>B. rochalimae<\/jats:italic> by targeting the intergenic transcribed spacer, <jats:italic>gltA<\/jats:italic>, and <jats:italic>rpoB<\/jats:italic> genes, which are recommended for subtyping characterization. This PCR platform achieved the limit of detection between five and 10 genomic equivalents per reaction and did not amplify DNA from other <jats:italic>Bartonella<\/jats:italic> species or selected hosts. This PCR platform is a fast and cost-effective option to be used in epidemiological evaluations of reservoirs and vectors and in detecting and quantifying <jats:italic>B. rochalimae<\/jats:italic> infection in humans.<\/jats:p>","DOI":"10.4269\/ajtmh.17-0740","type":"journal-article","created":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T07:00:32Z","timestamp":1533625232000},"page":"840-843","source":"Crossref","is-referenced-by-count":6,"title":["Bartonella rochalimae Detection by a Sensitive and Specific PCR Platform"],"prefix":"10.4269","volume":"99","author":[{"given":"Dennis","family":"Chan","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Western University of Health Sciences, Pomona, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph Andrew","family":"Geiger","sequence":"additional","affiliation":[{"name":"Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elton Jos\u00e9 Rosas","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Western University of Health Sciences, Pomona, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Oakley","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Western University of Health Sciences, Pomona, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro Paulo Vissotto de Paiva","family":"Diniz","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Western University of Health Sciences, Pomona, California;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Vet Res","first-page":"27","article-title":"Dogs are more permissive than cats or Guinea pigs to experimental infection with a human isolate of 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