{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T08:44:40Z","timestamp":1790239480890,"version":"4.1.0"},"reference-count":51,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,4,4]]},"abstract":"<jats:p>We assessed the ex vivo\/in vitro sensitivity of 54 Malian <jats:italic>Plasmodium falciparum<\/jats:italic> isolates to artemisinin for the monitoring of drug resistance in this area. The artemisinin sensitivity of parasites was evaluated using 1) the ex vivo and in vitro parasite recrudescence detection after treatment of the ring stage with 1\u2013200 nM artemisinin for 48 hours and 2) the in vitro parasite recrudescence kinetics assay over 7 days after 6-hour treatment of the ring stage with 700 nM dihydroartemisinin (DHA). In addition, as recommended by the World Health Organization for artemisinin resistance characterization, the ring-stage survival assay (RSA<jats:sup>0\u20133\u2009h<\/jats:sup>) was performed and the parasite isolates were sequenced at the kelch 13 propeller locus. No clinical and molecular evidence of artemisinin resistance was observed. However, these isolates present different phenotypic profiles in response to artemisinin treatments. Despite all RSA<jats:sup>0\u20133\u2009h<\/jats:sup> values less than 1.5%, six out of 46 (13.0%) isolates tested ex vivo and four out of six (66.7%) isolates tested in vitro were able to multiply after 48-hour treatments with 100 nM artemisinin. Moreover, five out of eight isolates tested showed faster parasite recovery after DHA treatment in kinetic assays. The presence of such phenotypes needs to be taken into account in the assessment of the efficacy of artemisinins in Mali. The assays presented here appear as valuable tools for the monitoring of artemisinin sensitivity in the field and thus could help to evaluate the risk of emergence of artemisinin resistance in Africa.<\/jats:p>","DOI":"10.4269\/ajtmh.17-0798","type":"journal-article","created":{"date-parts":[[2018,2,13]],"date-time":"2018-02-13T07:00:40Z","timestamp":1518505240000},"page":"1123-1131","source":"Crossref","is-referenced-by-count":12,"title":["Multiple Phenotypic and Genotypic Artemisinin Sensitivity Evaluation of Malian Plasmodium falciparum Isolates"],"prefix":"10.4269","volume":"98","author":[{"given":"Karamoko","family":"Niar\u00e9","sequence":"first","affiliation":[{"name":"Malaria Research and Training Center, University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Paloque","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie de Coordination du CNRS, CentreNationale de la Recherche Scientifique, Universit\u00e9 de Toulouse, UPS, INPT, Toulouse, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandie","family":"M\u00e9nard","sequence":"additional","affiliation":[{"name":"Centre de Physiopathologie de Toulouse Purpan, Universit\u00e9 de Toulouse, Toulouse, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pety","family":"Tor","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie de Coordination du CNRS, CentreNationale de la Recherche Scientifique, Universit\u00e9 de Toulouse, UPS, INPT, Toulouse, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arba P.","family":"Ramadani","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie de Coordination du CNRS, CentreNationale de la Recherche Scientifique, Universit\u00e9 de Toulouse, UPS, INPT, Toulouse, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Augereau","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie de Coordination du CNRS, CentreNationale de la Recherche Scientifique, Universit\u00e9 de Toulouse, UPS, INPT, Toulouse, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Dara","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali;"},{"name":"Division of Malaria Research, Institute for Global Health, University of Maryland School of Medicine, Baltimore, Maryland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Berry","sequence":"additional","affiliation":[{"name":"Centre de Physiopathologie de Toulouse Purpan, Universit\u00e9 de Toulouse, Toulouse, France;"},{"name":"Service de Parasitologie-Mycologie, Centre Hospitalier et Universitaire de Toulouse, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Benoit-Vical","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie de Coordination du CNRS, CentreNationale de la Recherche Scientifique, Universit\u00e9 de Toulouse, UPS, INPT, Toulouse, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ogobara K.","family":"Doumbo","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Center, University of Sciences, Techniques and Technologies of Bamako, Bamako, Mali;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Ann Trop Med Parasitol","first-page":"657","article-title":"What does it take to control malaria?","volume":"101","author":"Walther","year":"2007"},{"key":"b2","author":"WHO","year":"2016"},{"key":"b3","series-title":"N Engl J Med","first-page":"455","article-title":"Artemisinin 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