{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T20:01:59Z","timestamp":1776456119343,"version":"3.51.2"},"reference-count":36,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,12,5]]},"abstract":"<jats:p>Microsporidia are obligate intracellular parasitic fungi causing chronic diarrhea, particularly among immunocompromised patients. The current method used for diagnosis is based on conventional microscopy, which does not differentiate parasites at the species level. The present study was carried out to identify microsporidian species in immunocompromised patients. From March 2016 to March 2017, a total of 289 archived stool samples were examined microscopically for microsporidian spores using Gram-chromotrope Kinyoun (GCK) stain. Positive stool samples by microscopy were subjected to polymerase chain reaction and sequencing for species identification. Based on microscopy examination, the overall prevalence of microsporidian infections was 32.2% (93\/289; 95% CI = 27.1\u201337.8). Of these stool samples, 45 samples were successfully amplified and confirmed as <jats:italic>Enterocytozoon bieneusi<\/jats:italic>. No <jats:italic>Encephalitozoon intestinalis<\/jats:italic> was detected. Accurate identification of species might help clinicians to decide appropriate management strategies as dissemination risks and treatment response vary for different species, hence improving the management of microsporidian infections.<\/jats:p>","DOI":"10.4269\/ajtmh.17-0901","type":"journal-article","created":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T07:04:37Z","timestamp":1540883077000},"page":"1562-1566","source":"Crossref","is-referenced-by-count":9,"title":["Molecular Diagnosis of Microsporidia among Immunocompromised Patients in Kuala Lumpur, Malaysia"],"prefix":"10.4269","volume":"99","author":[{"given":"Nur-Amirah","family":"Hassan","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne A. L.","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"},{"name":"Centre of Excellence for Research in AIDS (CERIA), University of Malaya, Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohela","family":"Mahmud","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norashikin","family":"Mohd-Shaharuddin","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan Yusoff","family":"Wan Sulaiman","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romano","family":"Ngui","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","first-page":"186","author":"Cox","year":"2007"},{"key":"b2","series-title":"Acta Trop","first-page":"107","article-title":"Molecular identification of Enterocytozoon bieneusi and Encephalitozoon spp. in immunocompromised patients in Ahvaz, southwest of Iran","volume":"172","author":"Tavalla","year":"2017"},{"key":"b3","series-title":"J Acquir Immune Defic Syndr Hum Retrovirol","first-page":"154","article-title":"Declining CD4+ T-lymphocyte counts are associated with increased risk of enteric parasitosis and chronic diarrhea: results of a 3-year longitudinal study","volume":"20","author":"Navin","year":"1999"},{"key":"b4","series-title":"J 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