{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:32:51Z","timestamp":1790440371977,"version":"4.1.0"},"reference-count":20,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,6,6]]},"abstract":"<jats:p>The differential diagnosis of dengue virus (DENV) and yellow fever virus (YFV) infections in endemic areas is complicated by nonspecific early clinical manifestations. In this study, we describe an internally controlled, multiplex real-time reverse transcription polymerase chain reaction (rRT-PCR) for the detection of DENV and YFV. The DENV\u2013YFV assay demonstrated specific detection and had a dynamic range of 2.0\u20138.0 log<jats:sub>10<\/jats:sub> copies\/\u03bcL of eluate for each DENV serotype and YFV. Clinical performance was similar to a published pan-DENV assay: 48\/48 acute-phase samples from dengue cases were detected in both assays. For YFV detection, mock samples were prepared with nine geographically diverse YFV isolates over a range of concentrations. The DENV\u2013YFV assay detected 62\/65 replicates, whereas 54\/65 were detected using a reference YFV rRT-PCR. Given the reemergence of DENV and YFV in areas around the world, the DENV\u2013YFV assay should be a useful tool to narrow the differential diagnosis and provide early case detection.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0024","type":"journal-article","created":{"date-parts":[[2018,4,3]],"date-time":"2018-04-03T07:00:39Z","timestamp":1522738839000},"page":"1833-1836","source":"Crossref","is-referenced-by-count":19,"title":["Internally Controlled, Multiplex Real-Time Reverse Transcription PCR for Dengue Virus and Yellow Fever Virus Detection"],"prefix":"10.4269","volume":"98","author":[{"given":"Alejandra","family":"Rojas","sequence":"first","affiliation":[{"name":"Departamento de Producci\u00f3n, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, Asunci\u00f3n, Paraguay;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheikh T.","family":"Diagne","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, S\u00e9n\u00e9gal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria D.","family":"Stittleburg","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alisha","family":"Mohamed-Hadley","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine, Stanford, California;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvalena Ar\u00e9valo","family":"de Guill\u00e9n","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n, Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunci\u00f3n, Asunci\u00f3n, Paraguay;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Balmaseda","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional de Virolog\u00eda, Centro Nacional de Diagn\u00f3stico y Referencia, Ministry of Health, Managua, Nicaragua;"},{"name":"Sustainable Sciences Institute, Managua, Nicaragua;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumar","family":"Faye","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, S\u00e9n\u00e9gal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ousmane","family":"Faye","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, S\u00e9n\u00e9gal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amadou A.","family":"Sall","sequence":"additional","affiliation":[{"name":"Institut Pasteur de Dakar, Dakar, S\u00e9n\u00e9gal;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Harris","sequence":"additional","affiliation":[{"name":"Sustainable Sciences Institute, Managua, Nicaragua;"},{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin A.","family":"Pinsky","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine, Stanford, California;"},{"name":"Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse J.","family":"Waggoner","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia;"},{"name":"Department of Global Health, Rollins School of Public Health, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"J Clin Virol","first-page":"160","article-title":"Yellow 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