{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T11:24:19Z","timestamp":1772537059635,"version":"3.50.1"},"reference-count":18,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,9,5]]},"abstract":"<jats:p>The present study aims to develop a method for rapid diagnosis of malaria using loop-mediated isothermal amplification (LAMP) combined with a lateral flow device (LFD). By adding the biotin-labeled and fluorescein amidite-labeled loop primers to the LAMP reaction solution, the end product can be visualized on a LFD. The entire procedure takes approximately 42 minutes to complete, LAMP assay exhibited high sensitivity, as the detection limit was 0.01 pg\/\u03bcL for all five <jats:italic>Plasmodium<\/jats:italic> species. It was demonstrated that all <jats:italic>Plasmodium knowlesi<\/jats:italic> (<jats:italic>N<\/jats:italic> = 90) and <jats:italic>Plasmodium vivax<\/jats:italic> (<jats:italic>N<\/jats:italic> = 56) were positively amplified by LAMP-LFD assay, whereas healthy donor samples (<jats:italic>N<\/jats:italic> = 8) were negative. However, not all mixed infections were positive, and other infected nonmalaria samples were negative. Loop-mediated isothermal amplification-LFD represents a robust approach with potential suitability for use in resource-constrained laboratories. We believe that LAMP-LFD has a potential to be developed as point-of-care diagnostic tool in future.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0177","type":"journal-article","created":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T07:00:59Z","timestamp":1529996459000},"page":"704-708","source":"Crossref","is-referenced-by-count":11,"title":["Development of Loop-Mediated Isothermal Amplification\u2013Based Lateral Flow Device Method for the Detection of Malaria"],"prefix":"10.4269","volume":"99","author":[{"given":"Prudhvi Chand","family":"Mallepaddi","sequence":"first","affiliation":[{"name":"Genomix Molecular Diagnostics Pvt. Ltd., Hyderabad, India;"},{"name":"Department of Biotechnology, Acharya Nagarjuna University, Guntur, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng-Yee","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudhakar","family":"Podha","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Acharya Nagarjuna University, Guntur, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Choo-Huck","family":"Ooi","sequence":"additional","affiliation":[{"name":"Sarawak State Health Department, Jalan Diplomatik, Kuching, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan Wee-Kent","family":"Liew","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rathnagiri","family":"Polavarapu","sequence":"additional","affiliation":[{"name":"Genomix Molecular Diagnostics Pvt. Ltd., Hyderabad, India;"},{"name":"Department of Biotechnology, Acharya Nagarjuna University, Guntur, India;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yee-Ling","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","author":"WHO","year":"2017"},{"key":"b2","series-title":"Microbiol Aust","first-page":"39","article-title":"Plasmodium knowlesi: an update","volume":"37","author":"Singh","year":"2016"},{"key":"b3","series-title":"Nucleic Acids Res","first-page":"E63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000"},{"key":"b4","series-title":"Mol Cell Probes","first-page":"223","article-title":"Accelerated reaction by loop-mediated isothermal amplification using loop 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