{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T04:05:52Z","timestamp":1784433952466,"version":"3.55.0"},"reference-count":40,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,11,7]]},"abstract":"<jats:p>Cutaneous leishmaniasis is endemic in Salta province, which belongs to the northwest of Argentina. <jats:italic>Leishmania<\/jats:italic> spp. DNA from Giemsa-stained slides of up to 12 years in storage of patients from Salta was characterized through polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP). One hundred smears positive for microscopy, classified in a semiquantitative scale for amastigote density, were analyzed. Also, Leishmanin skin test (LST) results were included. DNA extraction was carried out applying lysis buffer with proteinase K, and then DNA was amplified with ribosomal internal transcribed spacer 1 primers. PCR products were digested with HaeIII enzyme. All PCR-positive smears (74\/100) belonged to <jats:italic>Viannia<\/jats:italic> subgenus. A statistically significant, directly proportional relationship between semiquantitative microscopy and PCR results was detected. All patients had LST-positive results (induration \u2265 5 mm), and the smears of those with smaller induration (LST &lt; 19 mm) gave a higher proportion of positive PCR results. This study determined that smear age did not affect PCR positivity, which allows retrospective analyzes and suggests smears might be useful for molecular complementary diagnosis. Because <jats:italic>Leishmania<\/jats:italic> (<jats:italic>Viannia<\/jats:italic>) <jats:italic>braziliensis<\/jats:italic> is the main circulating species in the study area, determining <jats:italic>Viannia<\/jats:italic> subgenus in all analyzed samples confirms previous findings. PCR positivity showed statistically significant differences according to semiquantitative microscopy, highlighting the importance of parasite burden in the diagnostic sensitivity of the method. Considering that smears of patients with smaller LST induration were more positive in PCR, a negative smear from patients with positive LST response, but &lt; 19 mm, could actually represent a false-negative result.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0223","type":"journal-article","created":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T07:00:44Z","timestamp":1537858844000},"page":"1156-1161","source":"Crossref","is-referenced-by-count":5,"title":["Molecular Identification of Leishmania spp. DNA from Archived Giemsa-Stained Slides of Patients from Salta, Argentina"],"prefix":"10.4269","volume":"99","author":[{"given":"Mar\u00eda Cristina","family":"Almaz\u00e1n","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"},{"name":"C\u00e1tedra de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Salta, Argentina;"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Salta, Argentina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Lorenzo","family":"Hoyos","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Salta, Argentina;"},{"name":"Instituto de Patolog\u00eda Experimental, CONICET, Salta, Argentina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro Javier","family":"Krolewiecki","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Salta, Argentina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana Pamela","family":"Cajal","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Griselda Noem\u00ed","family":"Copa","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"},{"name":"C\u00e1tedra de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Salta, Argentina;"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Salta, Argentina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Emanuel","family":"Fleitas","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"},{"name":"C\u00e1tedra de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Salta, Argentina;"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Salta, Argentina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola Andrea","family":"Barroso","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Salta, Argentina;"},{"name":"Instituto de Patolog\u00eda Experimental, CONICET, Salta, Argentina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Diego","family":"Marco","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Salta, Argentina;"},{"name":"Instituto de Patolog\u00eda Experimental, CONICET, Salta, Argentina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio Rub\u00e9n","family":"Nasser","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"},{"name":"C\u00e1tedra de Qu\u00edmica Biol\u00f3gica, Facultad de Ciencias Naturales, Universidad Nacional de Salta, Salta, Argentina;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Fernando","family":"Gil","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones de Enfermedades Tropicales, Universidad Nacional de Salta, Or\u00e1n, Argentina;"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Salta, Argentina;"},{"name":"Instituto de Investigaciones en Energ\u00eda No Convencional, CONICET, Salta, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Clin Microbiol Rev","first-page":"230","article-title":"Leishmaniases of the new world: current concepts and implications for future research","volume":"6","author":"Grimaldi","year":"1993"},{"key":"b2","series-title":"Parasitology","first-page":"430","article-title":"An appraisal of the taxonomy and nomenclature of trypanosomatids presently classified as Leishmania and Endotrypanum","volume":"145","author":"Espinosa","year":"2016"},{"key":"b3","series-title":"Comp Immunol Microbiol Infect Dis","first-page":"305","article-title":"Leishmaniasis: current situation and new perspectives","volume":"27","author":"Desjeux","year":"2004"},{"key":"b4","series-title":"Mem Inst Oswaldo Cruz","first-page":"282","article-title":"Urban distribution of phlebotominae in a cutaneous leishmaniasis focus, 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