{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:22:56Z","timestamp":1772583776190,"version":"3.50.1"},"reference-count":55,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,11,7]]},"abstract":"<jats:p>A clinical, serological, and molecular investigation was performed to determine the presence of dengue virus (DENV) and other flaviviruses among residents of the city of Reynosa, Tamaulipas, on the Mexico\u2013U.S. border in 2014\u20132016. The sample population consisted of 2,355 patients with suspected dengue, in addition to 346 asymptomatic individuals recruited during a household-based epidemiological investigation designed to identify flavivirus seroconversions. Sera were collected from patients with suspected dengue in the acute phase of illness and from asymptomatic individuals at enrollment and every 5\u20137 months for 19 months. Sera from suspected dengue patients were tested for DENV antigen by enzyme-linked immunosorbent assay (ELISA), and select antigen-positive sera were further tested using a serotype-specific, quantitative reverse transcription\u2013polymerase chain reaction. Sera from the household cohort were tested for flavivirus-reactive antibodies by immunoglobulin (Ig) M and IgG ELISAs using DENV antigen. A total of 418 (17.7%) patients with suspected dengue had laboratory-confirmed DENV infections, including 82 patients who were positive for DENV RNA. The most frequently detected serotype was DENV-1 (61 patients), followed by DENV-2 (16 patients) and DENV-3 (five patients). A total of 217 (62.7%) asymptomatic individuals had flavivirus-reactive antibodies at enrollment, and nine flavivirus-na\u00efve individuals seroconverted. Sera from a subset of dengue patients and household participants, including all those who seroconverted, were further tested by plaque reduction neutralization test, resulting in the detection of antibodies to DENV-1, DENV-2, and West Nile virus. In summary, we provide evidence for the co-circulation of multiple flaviviruses in Reynosa, Tamaulipas, on the Mexico\u2013U.S. border.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0426","type":"journal-article","created":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T07:01:07Z","timestamp":1537254067000},"page":"1308-1317","source":"Crossref","is-referenced-by-count":12,"title":["Surveillance for Flaviviruses Near the Mexico\u2013U.S. Border: Co-circulation of Dengue Virus Serotypes 1, 2, and 3 and West Nile Virus in Tamaulipas, Northern Mexico, 2014\u20132016"],"prefix":"10.4269","volume":"99","author":[{"given":"S. Viridiana","family":"Laredo-Tiscare\u00f1o","sequence":"first","affiliation":[{"name":"Laboratorio de Biomedicina Molecular, Centro de Biotecnolog\u00eda Gen\u00f3mica del Instituto Polit\u00e9cnico Nacional, Reynosa, Tamaulipas, M\u00e9xico;"},{"name":"Laboratorio de Arbovirolog\u00eda, Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"},{"name":"Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier A.","family":"Garza-Hernandez","sequence":"additional","affiliation":[{"name":"Laboratorio Entomolog\u00eda M\u00e9dica, Instituto de Ciencias Biom\u00e9dicas, Universidad Aut\u00f3noma de Ciudad Ju\u00e1rez, Ciudad Ju\u00e1rez, Chihuahua, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma Isabel","family":"Salazar","sequence":"additional","affiliation":[{"name":"Laboratorio de Virolog\u00eda e Inmunovirolog\u00eda, Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Ciudad de M\u00e9xico, Mexico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erick J.","family":"De Luna-Santillana","sequence":"additional","affiliation":[{"name":"Laboratorio Medicina de la Conservaci\u00f3n, Centro de Biotecnolog\u00eda Gen\u00f3mica del Instituto Polit\u00e9cnico Nacional, Reynosa, Tamaulipas, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chandra S.","family":"Tangudu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa C.","family":"Cetina-Trejo","sequence":"additional","affiliation":[{"name":"Laboratorio de Arbovirolog\u00eda, Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria L.","family":"Doria-Cobos","sequence":"additional","affiliation":[{"name":"Departamento de Epidemiolog\u00eda de la Cuarta Jurisdicci\u00f3n Sanitaria, Secretaria de Salud, Reynosa, Tamaulipas, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santos Daniel","family":"Carmona-Aguirre","sequence":"additional","affiliation":[{"name":"Secretar\u00eda de Salud del Estado de Tamaulipas, Direcci\u00f3n de Epidemiolog\u00eda, Ciudad Victoria, Tamaulipas, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian E.","family":"Garcia-Rejon","sequence":"additional","affiliation":[{"name":"Laboratorio de Arbovirolog\u00eda, Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Machain-Williams","sequence":"additional","affiliation":[{"name":"Laboratorio de Arbovirolog\u00eda, Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi,\u201d Universidad Aut\u00f3noma de Yucat\u00e1n, M\u00e9rida, M\u00e9xico;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley J.","family":"Blitvich","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Alberto Rodr\u00edguez","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biomedicina Molecular, Centro de Biotecnolog\u00eda Gen\u00f3mica del Instituto 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