{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T07:23:29Z","timestamp":1778743409447,"version":"3.51.4"},"reference-count":27,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,11,7]]},"abstract":"<jats:p>Carrion\u2019s disease is a neglected, vector-borne illness that affects Colombia, Ecuador, and especially Peru. The phlebotomine sand flies <jats:italic>Lutzomyia verrucarum<\/jats:italic> and <jats:italic>Lutzomyia peruensis<\/jats:italic> are the main illness vectors described, although other species may be implicated in endemic areas such as some northern Peruvian regions, in which Carrion\u2019s disease vector has not been established. The aim of this study was to evaluate the presence of <jats:italic>Bartonella bacilliformis<\/jats:italic> DNA in <jats:italic>Lutzomyia maranonensis<\/jats:italic> from Cajamarca, northern Peru. This sand fly has not been defined as a vector yet. Centers for Disease Control and Prevention light traps were used to collect adult phlebotomine sand flies from 2007 to 2008 in the Cajamarca department. Female specimens were identified using morphological keys and were grouped into pools of five sand flies, taking into account district and sampling site (intradomicile or peridomicile). DNA was extracted, and then conventional and real-time polymerase chain reaction (RT-PCR) were performed to detect <jats:italic>B. bacilliformis<\/jats:italic> and subsequently confirmed by sequencing. A total of 383 specimens of <jats:italic>L. maranonensis<\/jats:italic> species were analyzed. Two of 76 pools were positive for <jats:italic>B. bacilliformis<\/jats:italic> by sequencing; all positives pools were from Querocotillo district. In addition, <jats:italic>Mesorhizobium<\/jats:italic> spp. were identified in two pools of sand flies, which is an \u03b1-proteobacteria phylogenetically very close to <jats:italic>B. bacilliformis.<\/jats:italic> This study presents molecular evidence that suggests <jats:italic>L. maranonensis<\/jats:italic> is naturally infected by <jats:italic>B. bacilliformis<\/jats:italic> in the Cajamarca department. Further research should determine if <jats:italic>L. maranonensis<\/jats:italic> is a vector and could transmit <jats:italic>B. bacilliformis.<\/jats:italic><\/jats:p>","DOI":"10.4269\/ajtmh.18-0520","type":"journal-article","created":{"date-parts":[[2018,9,18]],"date-time":"2018-09-18T07:01:05Z","timestamp":1537254065000},"page":"1229-1233","source":"Crossref","is-referenced-by-count":16,"title":["Molecular Detection of Bartonella bacilliformis in Lutzomyia maranonensis in Cajamarca, Peru: A New Potential Vector of Carrion\u2019s Disease in Peru?"],"prefix":"10.4269","volume":"99","author":[{"given":"Gabriela M.","family":"Ulloa","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n e Innovaci\u00f3n, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas, Lima, Peru;"},{"name":"Emerge, Emerging Diseases and Climate Change Research Unit, School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima, Peru;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"V\u00e1squez-Achaya","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n e Innovaci\u00f3n, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas, Lima, Peru;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia","family":"Gomes","sequence":"additional","affiliation":[{"name":"ISGlobal, Hospital Cl\u00ednic\u2014Universitat de Barcelona, Barcelona, Spain;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis J.","family":"del Valle","sequence":"additional","affiliation":[{"name":"Centre de Recerca en Ci\u00e8ncia i Enginyeria Multiescala de Barcelona, Departament d\u2019Enginyeria Qu\u00edmica, Escuela de Ingenier\u00eda de Barcelona Este (EEBE), Universitat Polit\u00e8cnica de Catalunya (UPC) BarcelonaTech, Barcelona, Spain;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaquim","family":"Ruiz","sequence":"additional","affiliation":[{"name":"ISGlobal, Hospital Cl\u00ednic\u2014Universitat de Barcelona, Barcelona, Spain;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria J.","family":"Pons","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n e Innovaci\u00f3n, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas, Lima, Peru;"},{"name":"Laboratorio de Microbiolog\u00eda y Gen\u00f3mica Bacteriana, Universidad Cient\u00edfica del Sur, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juana","family":"del Valle Mendoza","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n e Innovaci\u00f3n, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas, Lima, Peru;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"PLoS Negl Trop Dis","first-page":"e1819","article-title":"Bartonella bacilliformis: a systematic review of the literature to guide the research agenda for elimination","volume":"6","author":"Sanchez Clemente","year":"2012"},{"key":"b2","author":"Pachas","year":"2001"},{"key":"b3","series-title":"PLoS Negl Trop Dis","first-page":"e2919","article-title":"Oroya fever and verruga peruana: bartonelloses unique to South America","volume":"8","author":"Minnick","year":"2014"},{"key":"b4","series-title":"PLoS Pathog","first-page":"e1005863","article-title":"Carrion\u2019s disease: more than a sand fly-vectored illness","volume":"12","author":"Pons","year":"2016"},{"key":"b5","series-title":"PLoS Negl Trop Dis","first-page":"e0004989","article-title":"Succinyl-CoA synthetase: new antigen candidate of Bartonella bacilliformis","volume":"10","author":"Gomes","year":"2016"},{"key":"b6","series-title":"Infect Dis Poverty","first-page":"105","article-title":"Carrion\u2019s disease: an eradicable illness?","volume":"5","author":"Gomes","year":"2016"},{"key":"b7","series-title":"Rev Peru Biol","first-page":"27","article-title":"Psychodidae (Diptera) del Per\u00fa I: phlebotominae en Hu\u00e1nuco, Pasco y Cusco, su relaci\u00f3n con la enfermedad de Carri\u00f3n y la leishmaniasis tegumentaria","volume":"7","author":"C\u00e1ceres","year":"2000"},{"key":"b8","author":"Romero","year":"2004"},{"key":"b9","series-title":"Rev Med Exp","first-page":"28","article-title":"Importancia de la Lutzomyia peruensis en la transmisi\u00f3n de la enfermedad de Carrion en el Valle Sagrado de los Incas. Urubamba-Cusco, Peru","volume":"15","author":"Villaseca","year":"1999"},{"key":"b10","series-title":"Med Vet Entomol","first-page":"123","article-title":"Shifts in the ecological niche of Lutzomyia peruensis under climate change scenarios in Peru","volume":"31","author":"Moo-Llanes","year":"2017"},{"key":"b11","series-title":"Rev Inst Med Trop S\u00e3o Paulo","first-page":"51","article-title":"Possible role of Lutzomyia maranonensis and Lutzomyia robusta (Diptera: Psychodidae) as vectors of human bartonellosis in three provinces of region nor Oriental del Mara\u00f1on, Peru","volume":"39","author":"Caceres","year":"1997"},{"key":"b12","series-title":"Rev Med Exp","first-page":"100","article-title":"Lista de phlebotominae (Diptera: psychodidae) para el Per\u00fa y especies consideradas como vectores naturales e incriminadas en la transmisi\u00f3n de pat\u00f3genos de la leishmaniosis tegumentaria y la enfermedad de Carri\u00f3n (verruga peruana)","volume":"18","author":"C\u00e1ceres","year":"2001"},{"key":"b13","author":"Ministerio de Salud","year":"2016"},{"key":"b14","author":"Municipalidad Provincial de Cutervo","year":"2015"},{"key":"b15","series-title":"Mem Am Entomol Inst","first-page":"1","article-title":"Guide to the identification and geographic distribution of Lutzomyia sand flies in Mexico, the West Indies, central in South America (Diptera: Psychodidade)","volume":"54","author":"Young","year":"1994"},{"key":"b16","series-title":"PLoS One","first-page":"e92283","article-title":"Diagnosis of Carrion\u2019s disease by direct blood PCR in thin blood smear negative samples","volume":"9","author":"del Valle Mendoza","year":"2014"},{"key":"b17","series-title":"Microbiol China","first-page":"427","article-title":"Detection of Bartonella bacilliformis by real-time PCR with TaqMan-MGB probe","volume":"42","author":"Dong-Mei","year":"2015"},{"key":"b18","series-title":"Acta Trop","first-page":"252","article-title":"Detection and identification of Leishmania species in field-captured phlebotomine sandflies based on mini-exon gene PCR","volume":"99","author":"Paiva","year":"2006"},{"key":"b19","series-title":"Acta Trop","first-page":"41","article-title":"Man-biting sand fly species and natural infection with the Leishmania promastigote in leishmaniasis-endemic areas of Ecuador","volume":"140","author":"Gomez","year":"2014"},{"key":"b20","series-title":"Clin Microbiol Rev","first-page":"e00056","article-title":"Carrion\u2019s disease: the sound of silence","volume":"31","author":"Gomes","year":"2018"},{"key":"b21","series-title":"Mem Inst Oswaldo Cruz","first-page":"277","article-title":"Association of Lutzomyia columbiana (Diptera: Psychodidae) with a leishmaniasis focus in Colombia due to species of the Leishmania mexicana complex","volume":"94","author":"Montoya-Lerma","year":"1999"},{"key":"b22","series-title":"PLoS Negl Trop Dis","first-page":"e0004529","article-title":"Evaluation of PCR approaches for detection of Bartonella bacilliformis in blood samples","volume":"10","author":"Gomes","year":"2016"},{"key":"b23","series-title":"PLoS One","first-page":"e42531","article-title":"Investigation of the bacterial communities associated with females of Lutzomyia sand fly species from South America","volume":"7","author":"Sant\u2019Anna","year":"2012"},{"key":"b24","series-title":"Neotrop Entomol","first-page":"597","article-title":"Study on the bacterial midgut microbiota associated to different Brazilian populations of Lutzomyia longipalpis (Lutz & Neiva) (Diptera: Psychodidae)","volume":"37","author":"Gouveia","year":"2008"},{"key":"b25","series-title":"Int J Syst Evol Microbiol","first-page":"414","article-title":"Bartonella apis sp. nov., a honey bee gut symbiont of the class Alphaproteobacteria","volume":"66","author":"Ke\u0161nerov\u00e1","year":"2016"},{"key":"b26","series-title":"PLoS Negl Trop Dis","first-page":"e0003789","article-title":"Competence of Cimex lectularius bed bugs for the transmission of Bartonella quintana, the agent of trench fever","volume":"9","author":"Leulmi","year":"2015"},{"key":"b27","series-title":"Environ Entomol","first-page":"1178","article-title":"Molecular phylogeny of the genus Dactylopius (Hemiptera: Dactylopiidae) and identification of the symbiotic bacteria","volume":"39","author":"Ram\u00edrez-Puebla","year":"2010"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/99\/5\/article-p1229.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/99\/5\/article-p1229.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/99\/5\/article-p1229.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T20:25:38Z","timestamp":1722457538000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/99\/5\/article-p1229.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,7]]},"references-count":27,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.18-0520","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,7]]}}}