{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T11:49:53Z","timestamp":1781264993278,"version":"3.54.1"},"reference-count":13,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,1,9]]},"abstract":"<jats:p>To evaluate the accuracy and reliability of urine assay for the diagnosis of strongyloidiasis, three different immunoassays were used to assess the diagnostic accuracy of anti-<jats:italic>Strongyloides<\/jats:italic> immunoglobulin G (IgG) in urine and compared with those in serum samples. Analyses by InBios enzyme-linked immunosorbent assay (ELISA) kit (recombinant NIE antigen), SciMedx ELISA kit (<jats:italic>Strongyloides stercoralis<\/jats:italic> antigen), and our in-house ELISA (<jats:italic>Strongyloides ratti<\/jats:italic> antigen) yielded comparable diagnostic performances between urine and serum assays. Levels of <jats:italic>Strongyloides<\/jats:italic>-specific IgG in urine significantly correlated with those in serum. Tests for diagnostic agreement between urine and serum IgG assays showed substantial to fair agreement (\u03ba = 0.207\u20130.615). The observed quantitative and qualitative concordance between urine and serum assays in strongyloidiasis suggests that urine has similar diagnostic value to that for serum. Because of the ease and noninvasiveness of clinical sample collection, urine assay has a high potential for the initial diagnosis and mass screening of strongyloidiasis.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0569","type":"journal-article","created":{"date-parts":[[2018,11,6]],"date-time":"2018-11-06T07:00:23Z","timestamp":1541487623000},"page":"127-129","source":"Crossref","is-referenced-by-count":16,"title":["Accuracy of Urine and Serum Assays for the Diagnosis of Strongyloidiasis by Three Enzyme-Linked Immunosorbent Assay Protocols"],"prefix":"10.4269","volume":"100","author":[{"given":"Sirowan","family":"Ruantip","sequence":"first","affiliation":[{"name":"Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chatanun","family":"Eamudomkarn","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anchalee","family":"Techasen","sequence":"additional","affiliation":[{"name":"Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chompunoot","family":"Wangboon","sequence":"additional","affiliation":[{"name":"Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiraporn","family":"Sithithaworn","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Bethony","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Tropical Medicine, George Washington University, Washington, District of Columbia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Itoh","sequence":"additional","affiliation":[{"name":"Department of Infection and Immunology, Aichi Medical University School of Medicine, Nagakute, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paiboon","family":"Sithithaworn","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Cholangiocarcinoma Research Institute (CARI), Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Trans R Soc Trop Med Hyg","first-page":"967","article-title":"Strongyloidiasis\u2014the most neglected of the neglected tropical diseases?","volume":"103","author":"Olsen","year":"2009"},{"key":"b2","series-title":"PLoS Negl Trop Dis","first-page":"e2288","article-title":"Strongyloides stercoralis: global distribution and risk factors","volume":"7","author":"Schar","year":"2013"},{"key":"b3","series-title":"PLoS Negl Trop Dis","first-page":"e2640","article-title":"Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection","volume":"8","author":"Bisoffi","year":"2014"},{"key":"b4","series-title":"Parasitol Res","first-page":"2543","article-title":"Comparative evaluation of Strongyloides ratti and S. stercoralis larval antigen for diagnosis of strongyloidiasis in an endemic area of opisthorchiasis","volume":"114","author":"Eamudomkarn","year":"2015"},{"key":"b5","series-title":"Am J Trop Med Hyg","first-page":"1048","article-title":"Real-time polymerase chain reaction for detection of Strongyloides stercoralis in stool","volume":"88","author":"Sultana","year":"2013"},{"key":"b6","series-title":"Am J Trop Med Hyg","first-page":"1285","article-title":"Comparison of nested polymerase chain reaction and real-time polymerase chain reaction with parasitological methods for detection of Strongyloides stercoralis in human fecal samples","volume":"93","author":"Sharifdini","year":"2015"},{"key":"b7","series-title":"Acta Trop","first-page":"9","article-title":"Diagnosis of Strongyloides stercoralis: detection of parasite-derived DNA in urine","volume":"163","author":"Lodh","year":"2016"},{"key":"b8","series-title":"PLoS Negl Trop Dis","first-page":"e0006550","article-title":"Transrenal DNA-based diagnosis of Strongyloides stercoralis (Grassi, 1879) infection: Bayesian latent class modeling of test accuracy","volume":"12","author":"Krolewiecki","year":"2018"},{"key":"b9","series-title":"PLoS One","first-page":"e0192598","article-title":"Diagnostic performance of urinary IgG antibody detection: a novel approach for population screening of strongyloidiasis","volume":"13","author":"Eamudomkarn","year":"2018"},{"key":"b10","series-title":"BMJ","first-page":"f4610","article-title":"Strongyloides stercoralis infection","volume":"347","author":"Greaves","year":"2013"},{"key":"b11","series-title":"Am J Trop Med Hyg","first-page":"518","article-title":"A modified agar plate method for detection of Strongyloides stercoralis","volume":"45","author":"Koga","year":"1991"},{"key":"b12","series-title":"Trans R Soc Trop Med Hyg","first-page":"398","article-title":"Epidemiology of Strongyloides stercoralis in north-east Thailand: application of the agar plate culture technique compared with the enzyme-linked immunosorbent assay","volume":"97","author":"Sithithaworn","year":"2003"},{"key":"b13","series-title":"Biometrics","first-page":"363","article-title":"An application of hierarchical kappa-type statistics in the assessment of majority agreement among multiple observers","volume":"33","author":"Landis","year":"1977"}],"container-title":["The American Journal of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/100\/1\/article-p127.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/100\/1\/article-p127.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/100\/1\/article-p127.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T11:19:12Z","timestamp":1722424752000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/100\/1\/article-p127.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,9]]},"references-count":13,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.18-0569","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,9]]}}}