{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T06:19:50Z","timestamp":1770617990783,"version":"3.49.0"},"reference-count":16,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,5,1]]},"abstract":"<jats:p>Leptospirosis is a neglected zoonotic bacterial disease caused by pathogenic <jats:italic>Leptospira<\/jats:italic> spp. Only limited studies have been conducted on the presence of <jats:italic>Leptospira<\/jats:italic> spp. in rats in Antananarivo, the capital city of Madagascar. We assessed <jats:italic>Leptospira<\/jats:italic> prevalence in small mammals in urban areas of Antananarivo where sanitation is inadequate and there is risk of flooding during the rainy season. We captured rodents and shrews at two sites and examined kidney samples from 114 animals using culture and a real-time polymerase chain reaction (PCR) assay specific to pathogenic <jats:italic>Leptospira<\/jats:italic> spp. We identified 23 positive samples containing <jats:italic>Leptospira interrogans<\/jats:italic> and <jats:italic>Leptospira borgpetersenii<\/jats:italic>, with a high prevalence in <jats:italic>Rattus norvegicus<\/jats:italic> (44.9%). Our results indicate that small mammals, in particular <jats:italic>R. norvegicus<\/jats:italic>, present a major public health risk for acquiring leptospirosis in Antananarivo.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0642","type":"journal-article","created":{"date-parts":[[2019,3,26]],"date-time":"2019-03-26T07:01:21Z","timestamp":1553583681000},"page":"1079-1081","source":"Crossref","is-referenced-by-count":12,"title":["High Prevalence of Leptospira spp. in Rodents in an Urban Setting in Madagascar"],"prefix":"10.4269","volume":"100","author":[{"given":"Soanandrasana","family":"Rahelinirina","sequence":"first","affiliation":[{"name":"Plague Unit, WHO Collaborating Center, Institut Pasteur, Antananarivo, Madagascar;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascale","family":"Bourhy","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biologie des Spiroch\u00e8tes, National Reference Centre for Leptospirosis, Institut Pasteur, Paris, France;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fehivola","family":"Andriamiaramanana","sequence":"additional","affiliation":[{"name":"Plague Unit, WHO Collaborating Center, Institut Pasteur, Antananarivo, Madagascar;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benoit","family":"Garin","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire, Les Abymes, Guadeloupe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoarisoa","family":"Rajerison","sequence":"additional","affiliation":[{"name":"Plague Unit, WHO Collaborating Center, Institut Pasteur, Antananarivo, Madagascar;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Clin Microbiol Rev","first-page":"296","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001"},{"key":"b2","series-title":"Lancet","first-page":"820","article-title":"Urban epidemic of severe leptospirosis in Brazil: Salvador leptospirosis study group","volume":"354","author":"Ko","year":"1999"},{"key":"b3","series-title":"PLoS Negl Trop Dis","first-page":"e228","article-title":"Impact of environment and social gradient on Leptospira infection in urban slums","volume":"2","author":"Reis","year":"2008"},{"key":"b4","series-title":"J Trav Med","first-page":"136","article-title":"Leptospirosis after a stay in Madagascar","volume":"22","author":"Pag\u00e8s","year":"2015"},{"key":"b5","series-title":"PLoS One","first-page":"e0122683","article-title":"Has Madagascar lost its exceptional leptospirosis free-like status?","volume":"10","author":"Ratsitorahina","year":"2015"},{"key":"b6","series-title":"PLoS One","first-page":"e14111","article-title":"First isolation and direct evidence for the existence of large small-mammal reservoirs of Leptospira sp. in Madagascar","volume":"5","author":"Rahelinirina","year":"2010"},{"key":"b7","series-title":"Mol 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NotI digests of leptospiral DNA: a new rapid method of serovar identification","volume":"30","author":"Herrmann","year":"1992"},{"key":"b12","series-title":"Lancet Infect Dis","first-page":"757","article-title":"Leptospirosis: a zoonotic disease of global importance","volume":"3","author":"Bharti","year":"2003"},{"key":"b13","series-title":"Emerg Infect Dis","first-page":"1138","article-title":"Mixed Leptospira infections in a diverse reservoir host community, Madagascar, 2013\u20132015","volume":"24","author":"Moseley","year":"2018"},{"key":"b14","series-title":"Adv Exp Med Biol","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/0-306-48416-1_87","article-title":"Susceptibility to plague of the rodents in Antananarivo, Madagascar","volume":"529","author":"Rahalison","year":"2003"},{"key":"b15","series-title":"Arch Inst Pasteur Madagascar","first-page":"19","article-title":"La peste dans le march\u00e9 Tsenabe Isotry \u00e0 Antananarivo: une situation 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