{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:07:08Z","timestamp":1791295628090,"version":"4.1.0"},"reference-count":35,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,2,6]]},"abstract":"<jats:p>Cystic echinococcosis (CE) is a parasitic zoonosis caused by the larval stage of the tapeworm <jats:italic>Echinococcus granulosus<\/jats:italic>. Detection of the adult stage in the canine definitive host is essential for estimating infection rates, surveillance and monitoring of CE control programs. This study sought to develop and validate a coproantigen sandwich enzyme\u2013linked immunosorbent assay (copro-ELISA), based on antibodies against <jats:italic>E. granulosus<\/jats:italic>\u2013soluble membrane antigens (EGMA), that is capable of distinguishing infected and noninfected dogs. Anti-<jats:italic>E. granulosus<\/jats:italic> polyclonal immunoglobulin G antibodies were obtained from rabbit antiserum against EGMA. Optimization of the test was performed with 51 positive and 56 negative stool samples of canine echinococcosis. Specificity, sensitivity, cross-reactivity, intra- and inter-assay precision, and over time detection were evaluated. According to the receiver operating characteristic analysis, the diagnostic sensitivity and specificity were 96.1% (CI: 85.9\u201399.6) and 98.2% (CI: 89.5\u2013100), respectively. Negative and positive predictive values were 96.5% (CI: 91.7\u2013100) and 98% (CI: 94.1\u2013100), respectively. No cross-reactivity with <jats:italic>Taenia hydatigena<\/jats:italic>, <jats:italic>Dipylidium caninum<\/jats:italic>, or <jats:italic>Toxocara canis<\/jats:italic> was observed. Intra- and inter-assay repeatability showed values of less than 15% of the variation coefficient. The over time detection was from 20 to 27 days postinfection with <jats:italic>E. granulosus<\/jats:italic>. The copro-ELISA based on EGMA detection offers a simplified in-house development of diagnostic testing. This assay showed high specificity and sensitivity and had no cross-reactivity with other parasites. Further studies and development of this test in a kit format may be useful for the detection of active infection in dogs living in CE endemic regions.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0645","type":"journal-article","created":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T07:01:03Z","timestamp":1544511663000},"page":"330-335","source":"Crossref","is-referenced-by-count":15,"title":["Development and Validation of a Copro-Enzyme\u2013Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus\u2013Soluble Membrane Antigens in Dogs"],"prefix":"10.4269","volume":"100","author":[{"given":"Luis M.","family":"Jara","sequence":"first","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Per\u00fa;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magaly","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias y Filosof\u00eda, Universidad Peruana Cayetano Heredia, Lima, Per\u00fa;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faride","family":"Altamirano","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per\u00fa;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Herrera","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per\u00fa;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Verastegui","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias y Filosof\u00eda, Universidad Peruana Cayetano Heredia, Lima, Per\u00fa;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis G.","family":"G\u00edmenez-Lirola","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Iowa State University, Ames, Iowa;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar M.","family":"Gavidia","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per\u00fa;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Am Fam Physician","first-page":"817","article-title":"Echinococosis-an emerging parasite in the immigrant population","volume":"66","author":"Chrieki","year":"2002"},{"key":"b2","series-title":"J Clin Microbiol","first-page":"518","article-title":"Cystic echinococcosis","volume":"54","author":"Agudelo Higuita","year":"2015"},{"key":"b3","series-title":"Rev Panam Salud Publica","first-page":"1","article-title":"Cystic 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