{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:12:31Z","timestamp":1789873951972,"version":"4.0.1"},"reference-count":40,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,3,6]]},"abstract":"<jats:p>The pig is the natural intermediate host of <jats:italic>Taenia solium<\/jats:italic>, a parasite causing significant burden of disease in both humans and pigs. Porcine cysticercosis is traditionally detected via tongue palpation and slaughterhouse meat inspection, both with limited sensitivity. Serum antibody detection has a better performance; however, it does not discriminate past from present infection. Serum antigen detection can demonstrate viable infection and gives a good estimate of parasitic load. This study evaluated a sandwich antigen-detection ELISA using monoclonal antibodies (MoAbs) 158C11 and 60H8 for the diagnosis of viable cysticercosis in pigs. Serum samples were used from 35 naturally <jats:italic>T. solium<\/jats:italic> cysticerci\u2013infected pigs, 31 cysticercosis-negative pigs, and 22 pigs with <jats:italic>Taenia hydatigena<\/jats:italic> infection (to assess cross-reactions). Positive cysticercosis samples were subcategorized at necropsy according to parasitic burden as mild (1\u201310 viable cysts, <jats:italic>n<\/jats:italic> = 10), moderate (11\u2013100 cysts, <jats:italic>n<\/jats:italic> = 5), or severe infection (more than 100 cysts, <jats:italic>n<\/jats:italic> = 20). This Ag-ELISA showed a sensitivity of 82.9% and a specificity of 96.8% when not considering cross-reactions with <jats:italic>T. hydatigena<\/jats:italic>. Hundred percentage of severely infected, 80% of moderately infected, and 50% of mildly <jats:italic>T. solium<\/jats:italic>\u2013infected pigs tested positive. Twenty of 22 pigs with only <jats:italic>T. hydatigena<\/jats:italic> infections were positive, with 13 reaching saturating levels in the ELISA. The Ag-ELISA revealed the presence of live cysts and is, thus, a fairly reliable test to monitor experimental infection, response to treatment, and follow-up in animal models of cysticercosis. It should, however, be carefully interpreted when used in regions where <jats:italic>T. hydatigena<\/jats:italic> is endemic in pigs.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0697","type":"journal-article","created":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T07:20:29Z","timestamp":1547536829000},"page":"604-608","source":"Crossref","is-referenced-by-count":21,"title":["Performance of a Sandwich Antigen-Detection ELISA for the Diagnosis of Porcine Taenia solium Cysticercosis"],"prefix":"10.4269","volume":"100","author":[{"given":"Javier A.","family":"Bustos","sequence":"first","affiliation":[{"name":"Department of Microbiology, School of Sciences and Center for Global Health\u2013Tumbes, Universidad Peruana Cayetano Heredia, Lima, Peru;"},{"name":"Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berenice E.","family":"Ninaquispe","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yesenia","family":"Castillo","sequence":"additional","affiliation":[{"name":"Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan Y.","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Medicine, Trinity College Dublin, Dublin, Ireland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Dorny","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine, Antwerp, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Gabri\u00ebl","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9ctor H.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Sciences and Center for Global Health\u2013Tumbes, Universidad Peruana Cayetano Heredia, Lima, Peru;"},{"name":"Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando E.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"_","family":"_","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Sciences and Center for Global Health\u2013Tumbes, Universidad Peruana Cayetano Heredia, Lima, Peru;"},{"name":"Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru;"},{"name":"School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru;"},{"name":"School of Medicine, Trinity College Dublin, Dublin, Ireland;"},{"name":"Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Lancet","first-page":"571","article-title":"Of pigs and people\u2014WHO prepares to battle 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