{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T07:44:22Z","timestamp":1772005462231,"version":"3.50.1"},"reference-count":17,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,2,6]]},"abstract":"<jats:p>Neurocysticercosis (NCC), caused by <jats:italic>Taenia solium<\/jats:italic> larvae that reside in the central nervous system, results in serious public health and medical issues in many regions of the world. Current diagnosis of NCC is complex requiring both serology and costly neuroimaging of parasitic cysts in the brain. This diagnostic pipeline can be problematic in resource-constrained settings. There is an unmet need for a highly sensitive and clinically informative diagnostic test to complement the present diagnostic approaches. Here, we report that <jats:italic>T. solium<\/jats:italic>\u2013derived cell-free DNA is readily detectable in the urine of patients with the subarachnoid and parenchymal forms of NCC, and discuss the potential utility of this approach in enhancing and refining <jats:italic>T. solium<\/jats:italic> diagnostics.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0706","type":"journal-article","created":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T07:00:58Z","timestamp":1544511658000},"page":"327-329","source":"Crossref","is-referenced-by-count":16,"title":["Detection of Taenia solium DNA in the Urine of Neurocysticercosis Patients"],"prefix":"10.4269","volume":"100","author":[{"given":"Luz","family":"Toribio","sequence":"first","affiliation":[{"name":"Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miryam","family":"Romano","sequence":"additional","affiliation":[{"name":"W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan L.","family":"Scott","sequence":"additional","affiliation":[{"name":"W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isidro","family":"Gonzales","sequence":"additional","affiliation":[{"name":"Cysticercosis Unit, Instituto Nacional de Ciencias Neurol\u00f3gicas, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert","family":"Saavedra","sequence":"additional","affiliation":[{"name":"Cysticercosis Unit, Instituto Nacional de Ciencias Neurol\u00f3gicas, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hector H.","family":"Garcia","sequence":"additional","affiliation":[{"name":"Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;"},{"name":"Cysticercosis Unit, Instituto Nacional de Ciencias Neurol\u00f3gicas, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clive","family":"Shiff","sequence":"additional","affiliation":[{"name":"W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"_","family":"_","sequence":"additional","affiliation":[{"name":"Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru;"},{"name":"W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland;"},{"name":"Cysticercosis Unit, Instituto Nacional de Ciencias Neurol\u00f3gicas, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Lancet Neurol","first-page":"1202","article-title":"Clinical symptoms, diagnosis and treatment of neurocysticercosis","volume":"13","author":"Garcia","year":"2014"},{"key":"b2","series-title":"Trans R Soc Trop Med Hyg","first-page":"673","article-title":"Serum antigen detection in the diagnosis, treatment, and follow-up of neurocysticercosis patients","volume":"94","author":"Garcia","year":"2000"},{"key":"b3","series-title":"Trans R Soc Trop Med Hyg","first-page":"270","article-title":"Taenia solium cysticercosis in a village in northern Viet Nam: seroprevalence study using an ELISA for detecting circulating antigen","volume":"96","author":"Erhart","year":"2002"},{"key":"b4","series-title":"J Infect Dis","first-page":"50","article-title":"An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium)","volume":"159","author":"Tsang","year":"1989"},{"key":"b5","series-title":"Biochemia Med","first-page":"24","article-title":"Free DNA\u2014new potential analyte in clinical laboratory diagnostics?","volume":"22","author":"Wagner","year":"2012"},{"key":"b6","series-title":"Trends Parasitol","first-page":"378","article-title":"Cell-free DNA as a diagnostic tool for human parasitic infections","volume":"32","author":"Weerakoon","year":"2016"},{"key":"b7","series-title":"Lab Med","first-page":"6","article-title":"Recent advances in clinical applications of circulating cell-free DNA integrity","volume":"45","author":"Yu","year":"2014"},{"key":"b8","series-title":"J Clin Microbiol","first-page":"1283","article-title":"Isolation and characterization of species-specific DNA probes from Taenia solium and Taenia saginata and their use in an egg detection assay","volume":"33","author":"Chapman","year":"1995"},{"key":"b9","series-title":"PLoS Negl Trop Dis","first-page":"e1464","article-title":"Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis","volume":"6","author":"Ibironke","year":"2012"},{"key":"b10","series-title":"PLoS Negl Trop Dis","first-page":"e0006550","article-title":"Transrenal DNA-based diagnosis of Strongyloides stercoralis (Grassi, 1879) infection: Bayesian latent class modeling of test accuracy","volume":"12","author":"Krolewiecki","year":"2018"},{"key":"b11","series-title":"Acta Trop","first-page":"9","article-title":"Diagnosis of Strongyloides stercoralis: detection of parasite-derived DNA in urine","volume":"163","author":"Lodh","year":"2016"},{"key":"b12","series-title":"Am J Trop Med 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