{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:32:05Z","timestamp":1761514325865},"reference-count":17,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,3,6]]},"abstract":"<jats:p>Mitochondrial genome analysis of <jats:italic>Schistosoma japonicum<\/jats:italic> suggests that diversity of intermediate host snails drove intra-species divergence during its expansion in Asia. We applied the knowledge of this genomic variation to study an unusual patient we recently diagnosed with schistosomiasis. The patient had not visited any schistosomiasis-endemic countries for more than 35 years and had no idea where she became infected. Unusual clinical features of this patient included the absence of egg granulomas in tissue and persistent noncalcified eggs despite multiple praziquantel (PZQ) treatments over 7 years. A digital droplet polymerase chair reaction (PCR) assay that specifically targets the schistosome 1,4 dihydronicotinamide adenine dinucleotide-1 (NADH1) dehydrogenase-1 mitochondrial gene successfully amplified parasite DNA extracted from colon biopsies. DNA sequence analysis of parasite DNA revealed that it was a Philippine strain of <jats:italic>S. japonicum<\/jats:italic>. Future molecular studies using stored DNA from patients such as this may provide new insight into why some persons do not respond well to PZQ treatment.<\/jats:p>","DOI":"10.4269\/ajtmh.18-0752","type":"journal-article","created":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T07:42:58Z","timestamp":1546933378000},"page":"584-587","source":"Crossref","is-referenced-by-count":8,"title":["Persistence of Schistosoma japonicum DNA in a Kidney\u2013Liver Transplant Recipient"],"prefix":"10.4269","volume":"100","author":[{"given":"Michael","family":"Kron","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Gordon","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Australia;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Bauers","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zouyan","family":"Lu","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheran","family":"Mahatme","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janaki","family":"Shah","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kia","family":"Saeian","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology, Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald P.","family":"McManus","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Australia;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2795","reference":[{"key":"b1","series-title":"Lancet","first-page":"2253","article-title":"Human schistosomiasis","volume":"28","author":"Colley","year":"2014"},{"key":"b2","series-title":"Nat Rev Dis Primers","first-page":"13","article-title":"Schistosomiasis","volume":"4","author":"McManus","year":"2018"},{"key":"b3","series-title":"Scand J Infect Dis","first-page":"572","article-title":"Prolonged latent schistosomiasis diagnosed 38 years after infestation in a HIV patient","volume":"38","author":"Payet","year":"2006"},{"key":"b4","series-title":"J Infect Dis","first-page":"429","article-title":"Rodents as natural hosts of zoonotic Schistosoma species and hybrids: an epidemiological and evolutionary perspective from west Africa","volume":"218","author":"Catalano","year":"2018"},{"key":"b5","series-title":"J Infect Dis","first-page":"344","article-title":"New tools for old questions: how strictly human are \u201chuman schistosomes\u201d-and does it matter?","volume":"218","author":"Colley","year":"2018"},{"key":"b6","series-title":"Sci Rep","first-page":"18058","article-title":"Co-dispersal of the blood fluke Schistosoma japonicum and Homo sapiens in the neolithic age","volume":"5","author":"Yin","year":"2015"},{"key":"b7","series-title":"Sci Rep","first-page":"17345","article-title":"Exploring molecular variation in Schistosoma japonicum in 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