{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:32:49Z","timestamp":1787031169104,"version":"build-2736575974"},"reference-count":44,"publisher":"American Society of Tropical Medicine and Hygiene","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,3,6]]},"abstract":"<jats:p>\n                    Fascioliasis, caused by\n                    <jats:italic>Fasciola hepatica<\/jats:italic>\n                    and\n                    <jats:italic>Fasciola gigantica<\/jats:italic>\n                    infection, is a major food-borne trematodiasis in many places of the world, with the central region of Vietnam being reported as a highly endemic area. Stool examination for\n                    <jats:italic>Fasciola<\/jats:italic>\n                    eggs is not a sensitive method, and immunodiagnostic methods are preferable. We investigated various enzyme-linked immunosorbent assays (ELISAs) to evaluate their efficacy for fascioliasis diagnosis. Test sera used are primarily screened using an ELISA kit produced in Vietnam (VN kit; Viet Sinh Chemical Producing &amp; Trading Co. Ltd., Ho Chi Minh City, Vietnam): Seropositive individuals having symptoms compatible with fascioliasis were regarded as clinically diagnosed fascioliasis cases. A commercial\n                    <jats:italic>Fasciola<\/jats:italic>\n                    IgG ELISA kit from Diagnostic Automation\/Cortez Diagnostics, Inc. (USA kit; Woodland Hills, CA), which has been commonly used in Vietnam, was assessed and compared with in-house ELISA systems, including a cystatin-capture (CC) ELISA using crude worm extract (CWE) and an indirect ELISA using a synthetic peptide Ac-TPTCHWECQVGYNKTYDEE-NHMe designed from the\n                    <jats:italic>F. gigantica<\/jats:italic>\n                    cathepsin B (FgCB5) molecule. The USA kit was suitable for routine diagnosis after recalibration of the manufacturer\u2019s suggested cutoff point. Cystatin-capture ELISA with CWE provided good sensitivity and specificity with perfect agreement to the results of the USA kit. In dot-blot ELISA, recombinant FgCB5 reacted more strongly with human antisera than did other\n                    <jats:italic>F. gigantica<\/jats:italic>\n                    antigens tested. Enzyme-linked immunosorbent assay using the synthetic peptide fragment of the FgCB5 exhibited nearly 80% sensitivity and specificity, but the test results showed low agreement with CC-ELISA or the USA kit. In conclusion, the commercially available\n                    <jats:italic>Fasciola<\/jats:italic>\n                    IgG ELISA kit from the United States and the in-house CC ELISA using CWE are suitable for practical diagnosis for fascioliasis.\n                  <\/jats:p>","DOI":"10.4269\/ajtmh.18-0833","type":"journal-article","created":{"date-parts":[[2019,1,22]],"date-time":"2019-01-22T02:23:54Z","timestamp":1548123834000},"page":"591-598","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay Kit and In-House Fasciola gigantica Cysteine Proteinases-Based Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Fascioliasis"],"prefix":"10.4269","volume":"100","author":[{"given":"Na T. D.","family":"Tran","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"},{"name":"Department of Parasitology, Hue University of Medicine and Pharmacy, Hue, Vietnam;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phuong Anh","family":"Ton Nu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Hue University of Medicine and Pharmacy, Hue, Vietnam;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kitti","family":"Intuyod","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ly T. K.","family":"Dao","sequence":"additional","affiliation":[{"name":"Institute of Malariology, Parasitology and Entomology Quy Nhon, Quy Nhon, Vietnam;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Porntip","family":"Pinlaor","sequence":"additional","affiliation":[{"name":"Faculty of Associated Medical Sciences, Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukifumi","family":"Nawa","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Tropical Diseases Research Centre, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiattawee","family":"Choowongkomon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amornrat","family":"Geadkaew-Krenc","sequence":"additional","affiliation":[{"name":"Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanthawat","family":"Kosa","sequence":"additional","affiliation":[{"name":"Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudi","family":"Grams","sequence":"additional","affiliation":[{"name":"Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somchai","family":"Pinlaor","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2795","reference":[{"key":"b1","author":"World Health Organization (WHO) Ntd","year":"2018"},{"key":"b2","series-title":"Adv Parasitol","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0065-308X(09)69002-3","article-title":"Chapter 2. 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of Tropical Medicine and Hygiene"],"original-title":[],"link":[{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/100\/3\/article-p591.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/100\/3\/article-p591.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/www.ajtmh.org\/downloadpdf\/journals\/tpmd\/100\/3\/article-p591.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T16:49:21Z","timestamp":1722444561000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ajtmh.org\/view\/journals\/tpmd\/100\/3\/article-p591.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,6]]},"references-count":44,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.4269\/ajtmh.18-0833","relation":{},"ISSN":["0002-9637","1476-1645"],"issn-type":[{"value":"0002-9637","type":"print"},{"value":"1476-1645","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,6]]}}}